GCW_03245

Uniprot: A0A0F6CL60
Description: DNA polymerase IV
EC number: 2.7.7.7
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01113, ECO:0000256|SAAS:SAAS00498106}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01113}; ; Note=Binds 2 magnesium ions per subunit. {ECO:0000256|HAMAP-Rule:MF_01113}
Enzyme regulation:
Function [CC]: FUNCTION: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII. {ECO:0000256|HAMAP-Rule:MF_01113}.
Pathway:
Active site: ACT_SITE 109 109 {ECO:0000256|HAMAP-Rule:MF_01113}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 13 13 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01113}.; METAL 108 108 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01113}.
Nucleotide binding:
Site: SITE 18 18 Substrate discrimination. {ECO:0000256|HAMAP-Rule:MF_01113}.
Gene names (primary): umuC
Gene names (synonym): dinB
Mass: 46,890
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01113}.
Gene ontology (GO): cytoplasm [GO:0005737]; damaged DNA binding [GO:0003684]; DNA-directed DNA polymerase activity [GO:0003887]; magnesium ion binding [GO:0000287]; DNA repair [GO:0006281]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0000287; GO:0003684; GO:0003887; GO:0005737; GO:0006261; GO:0006281
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DNA polymerase type-Y family. {ECO:0000256|HAMAP-Rule:MF_01113}.; SIMILARITY: Contains 1 umuC domain. {ECO:0000256|HAMAP-Rule:MF_01113}.
Protein families: DNA polymerase type-Y family
Coiled coil:
Domain [FT]: DOMAIN 9 192 UmuC. {ECO:0000259|PROSITE:PS50173}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99832
UniPathway: K02346
CDD:
Gene3D:
HAMAP: 3.30.1490.100
InterPro: MF_01113
PANTHER: IPR017961;IPR022880;IPR001126
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50173
ProDom: PF00817;PF11799
SMART:
SUPFAM:
TIGRFAMs: SSF100879
780465-781671(+)

>nucleotide sequence
TTGTTTTTAATGGACAGAAAAACCATTTTTCATATCGATTTTGACGCCTTTTTTGCATCT
GTCGAAGAAAATTTTAACCCTGAGTATAATAACAAACCGTTAGTGGTTGGATCCAAGTCT
AATGGTTCTATCGTATCTAGTGCTAACTATATAGCTAGAAAATTTGGCGTTCGGTCAGCA
ATGCCTATTTTTCAAGCTAAAAAGTTATGTCCTTCTTTAATAATAGCTGAAGTTGACTAT
CCTAAATATGAAAGGGTGTCAGCTTATGTTTTTTCTTATTTAAGAGATTTTGTCTCTAAT
AAAATGGAAGTTGCTTCAATTGATGAATGCTATATGGACGTAACTGATATCTTAGAGAAG
AATAGTGAGATATCTGCAAGCGATTTTGCTAAAAAGATCCAAAAACAAATTTATGAGTTA
ACTCAATTAACTGTATCAATTGGCATCAGCTCAAATTTATTTTTAGCTAAGATGGCTTCT
GATCAGAACAAACCAAATGGAGTATATGAGATTTGACCTGATGAAATTGAAGAAAAATTA
TGACCACTTGAAATTAAGAAAATGTATTTAATAGGGTCTAAAAAAATACCTCTTCTAAAT
CAATTGAATATCAAAAACATTGGTAATTTTGCACAATTTGAGAATAAAGAATTATTAATT
GATATTTTTAAAAATATGTATTGAGATCACTATAATCACGCTCATGGAAAAGGTAGTGAT
TTTGTAGATTATGAAAGAAATTCACGAAAATCGATCTCTGTTTCAAAGAACATTAGACAC
AAAGTTAGTGATTATGAATCTTTATTAAAACTATTTAATGATTTATTTGATGACGTTTAT
TCAAGACTAAAAAACCATAATTTGTTAGCTAAAAACATATCGGTTTCAATAAGAACTGAA
AAAGTTAGATCGTTATCATTTAGTTTTAAACAATATAGCGATAAAAAAACCTTGTTTTAC
AACAAGGCTATAGATTTATTCGAGAGATTGCATAATGACAAATTAGTTGCTAACATCAGT
ATTTCGTTTGGAAACATTACAAACAAATACAAATTTATGCCCAACATTAATATCTATGAA
GAATTATCTGAAGAACAAAAAGAGACAATGTTACTAAAGAAAATTGTTAATCAAATTAAT
AAAGAATATAACAAAGAATTAGTTAATATTGCCGATGATTTTGATTATTTTAAATTTCAG
AAATAA

>protein sequence
LFLMDRKTIFHIDFDAFFASVEENFNPEYNNKPLVVGSKSNGSIVSSANYIARKFGVRSA
MPIFQAKKLCPSLIIAEVDYPKYERVSAYVFSYLRDFVSNKMEVASIDECYMDVTDILEK
NSEISASDFAKKIQKQIYELTQLTVSIGISSNLFLAKMASDQNKPNGVYEIWPDEIEEKL
WPLEIKKMYLIGSKKIPLLNQLNIKNIGNFAQFENKELLIDIFKNMYWDHYNHAHGKGSD
FVDYERNSRKSISVSKNIRHKVSDYESLLKLFNDLFDDVYSRLKNHNLLAKNISVSIRTE
KVRSLSFSFKQYSDKKTLFYNKAIDLFERLHNDKLVANISISFGNITNKYKFMPNINIYE
ELSEEQKETMLLKKIVNQINKEYNKELVNIADDFDYFKFQK