GCW_03265

Uniprot: A0A0F6CL63
Description: tRNA pseudouridine synthase A
EC number: 5.4.99.12
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-40). {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023484}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|SAAS:SAAS00023500}.
Pathway:
Active site: ACT_SITE 73 73 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|PIRSR:PIRSR001430-1}.
Binding site: BINDING 131 131 Substrate. {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|PIRSR:PIRSR001430-2}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): truA
Gene names (synonym):
Mass: 31,020
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}.
Gene ontology (GO): pseudouridine synthase activity [GO:0009982]; RNA binding [GO:0003723]; tRNA pseudouridine synthesis [GO:0031119]
Gene ontology IDs: GO:0003723; GO:0009982; GO:0031119
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family. {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00543779}.
Protein families: TRNA pseudouridine synthase TruA family
Coiled coil:
Domain [FT]: DOMAIN 30 124 PseudoU_synth_1. {ECO:0000259|Pfam:PF01416}.; DOMAIN 165 263 PseudoU_synth_1. {ECO:0000259|Pfam:PF01416}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99835
UniPathway: K06173
CDD:
Gene3D:
HAMAP: 3.30.70.580;3.30.70.660
InterPro: MF_00171
PANTHER: IPR020103;IPR001406;IPR020097;IPR020095;IPR020094
PIRSF: PTHR11142
PRINTS: PIRSF001430
PROSITE:
Pfam:
ProDom: PF01416
SMART:
SUPFAM:
TIGRFAMs: SSF55120
783307-784099(-)

>nucleotide sequence
TTATTCATAATAAACTTCATATAAGTACAACCCACTAGCAGGAGCTTTAGTTATTGCTCT
ACCTTTTTTGGGATTATCTAATAACCACTTCAAGTGATTAAGATCATATTTCTTAATCCC
GATATTATAAAGCGCACCTACAATCATTCTTACCATCGATCTTAAAAACCCATCACCCGT
GATATAAATATAAACCTTATTATCTTCTTGTTTCAGATTAATCTTATTGATTGTTCTAAG
ACCCTTATCTTTATCAGCTGTTGAGAACGATCAAAAATTATGTGTTCCTTCAAGGATTGG
GATGGCTTTTTCAATTAGATTAAAATCTAATTGAAAATTAACAATCCAAGCATGGTCATA
AAATAAAGGATTGTTAGCACAATAATCGATTAGATAAAGGTATGTTTTAGCTTTAGCACT
AAACCTAGCATGGAAATTACGATCAACTTCTTTGATTCATTTAATGTATCACTTATTTTG
ACCATAACGGTTTAAGATCTCTCTTAGTTTTTCTAAACTGATTCGATCATCAATATAAAA
ACTGATGTATTGATTGATCGCATGAACACCCTTATCCGTTCTACCACTAGCATTAACATA
GATCTTGGATTCATAGATCCATGCTAGTGCATCCTGTAATTTCGATTGAACTGTTTCATA
ATCATTTTGAACTGCATAACCATGGAACTTTGTTCCATGGTAAGAAATATTGATTAAAAC
GTTCTTATTTAACTTGGCCAACGTTTTCACCCAAACCGATCGTCATTCATTCTGGTGAAC
CAAACACACCAA

>protein sequence
LVCLVHQNEWRSVWVKTLAKLNKNVLINISYHGTKFHGYAVQNDYETVQSKLQDALAWIY
ESKIYVNASGRTDKGVHAINQYISFYIDDRISLEKLREILNRYGQNKWYIKWIKEVDRNF
HARFSAKAKTYLYLIDYCANNPLFYDHAWIVNFQLDFNLIEKAIPILEGTHNFWSFSTAD
KDKGLRTINKINLKQEDNKVYIYITGDGFLRSMVRMIVGALYNIGIKKYDLNHLKWLLDN
PKKGRAITKAPASGLYLYEVYYE