GCW_03290

Uniprot: A0A0F6CL68
Description: triosephosphate isomerase
EC number: 5.3.1.1
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate = glycerone phosphate. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00525314}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P). {ECO:0000256|HAMAP-Rule:MF_00147}.
Pathway: PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498179}.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498183}.
Active site: ACT_SITE 97 97 Electrophile. {ECO:0000256|HAMAP-Rule:MF_00147}.; ACT_SITE 169 169 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00147}.
Binding site: BINDING 175 175 Substrate; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00147}.; BINDING 215 215 Substrate. {ECO:0000256|HAMAP-Rule:MF_00147}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): tpiA
Gene names (synonym):
Mass: 27,920
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00498221}.
Gene ontology (GO): cytoplasm [GO:0005737]; triose-phosphate isomerase activity [GO:0004807]; gluconeogenesis [GO:0006094]; glycolytic process [GO:0006096]; pentose-phosphate shunt [GO:0006098]
Gene ontology IDs: GO:0004807; GO:0005737; GO:0006094; GO:0006096; GO:0006098
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the triosephosphate isomerase family. {ECO:0000256|HAMAP-Rule:MF_00147, ECO:0000256|RuleBase:RU363013, ECO:0000256|SAAS:SAAS00541575}.
Protein families: Triosephosphate isomerase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 11 13 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00147}.; REGION 236 237 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00147}.
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99840
UniPathway: K01803
CDD:
Gene3D: cd00311
HAMAP: 3.20.20.70
InterPro: MF_00147_B
PANTHER: IPR013785;IPR022896;IPR000652;IPR020861
PIRSF: PTHR21139
PRINTS:
PROSITE:
Pfam: PS00171;PS51440
ProDom: PF00121
SMART:
SUPFAM:
TIGRFAMs: SSF51351
788515-789274(-)

>nucleotide sequence
TTATTTAGCTTCAATAAGTTGGATAAATTTGTGTGGGTCTAATGAAGCTCCACCCACTAG
TGCTCCATTAATGTCTTTTTGTGCTAACAGTTCTGAAACATTATCAGGAGTAACTGATCC
ACCATATAAAATTGGTAACTCATTTGCGATCGTTTTTGATGATAATGAACCTAGAACAGT
TCGAATATAAGCGATTGCATCTTGCGCAGTTTGACTTGAAGCAGTCTTACCTGTTCCGAT
CGCCCAAACTGGTTCATAAGCAATAATTAGATCTTTTAATAAAGAACTACCAATTCCTTT
AAGATCTTTTTCTAACTGATCTTTAAGTACAGCTTTGGTCTTGTTAGCTTCGTATTCTTC
TAACGTTTCACCAATACATAAAATTGGCCTCATCCTGTTTTCTAATAAGGTTTTTACTTT
CTGATTAATTAATTCATCAGATTCGTGAAATAATTTTCTTCGTTCAGAGTGACCAATTAT
TACGTGATTAACACCAATATCTTTTAATTGTTCATAACTAACAGTTCCCGTGTTAGCTTT
ATTCATTACATAATTAGCATCTTGAGCTAAAACTAAACATTCGTTTCTTTTTAATTCATT
AGCCAAAGCCAAATGTACGAATACTGGTGCAACACCAAAAACCACGTCTGGGTTTTTGGT
TAACTTAGCTTGATTTAAAACACTAAAAAATTCTTTAACTTCTTGAGTAGTTTTATATGT
TTTTCAATTTCCAAAGATATATCTTTTCATACTGTTCAT

>protein sequence
MNSMKRYIFGNWKTYKTTQEVKEFFSVLNQAKLTKNPDVVFGVAPVFVHLALANELKRNE
CLVLAQDANYVMNKANTGTVSYEQLKDIGVNHVIIGHSERRKLFHESDELINQKVKTLLE
NRMRPILCIGETLEEYEANKTKAVLKDQLEKDLKGIGSSLLKDLIIAYEPVWAIGTGKTA
SSQTAQDAIAYIRTVLGSLSSKTIANELPILYGGSVTPDNVSELLAQKDINGALVGGASL
DPHKFIQLIEAK