GCW_03405

Uniprot: A0A0F6CL87
Description: Holliday junction DNA helicase RuvB
EC number: 3.6.4.12
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. {ECO:0000256|HAMAP-Rule:MF_00016}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. {ECO:0000256|HAMAP-Rule:MF_00016}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 43 50 ATP. {ECO:0000256|HAMAP-Rule:MF_00016}.
Site:
Gene names (primary): ruvB
Gene names (synonym):
Mass: 35,243
Subunit structure [CC]: SUBUNIT: Forms a complex with RuvA. {ECO:0000256|HAMAP-Rule:MF_00016}.
Gene ontology (GO): ATP binding [GO:0005524]; DNA binding [GO:0003677]; four-way junction helicase activity [GO:0009378]; DNA recombination [GO:0006310]; DNA repair [GO:0006281]; SOS response [GO:0009432]
Gene ontology IDs: GO:0003677; GO:0005524; GO:0006281; GO:0006310; GO:0009378; GO:0009432
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RuvB family. {ECO:0000256|HAMAP-Rule:MF_00016}.
Protein families: RuvB family
Coiled coil:
Domain [FT]: DOMAIN 35 168 AAA. {ECO:0000259|SMART:SM00382}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99859
UniPathway: K03551
CDD:
Gene3D:
HAMAP: 1.10.10.10;3.40.50.300
InterPro: MF_00016
PANTHER: IPR003593;IPR004605;IPR008823;IPR027417;IPR008824;IPR011991
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF05491;PF05496
SMART: PD005323
SUPFAM: SM00382
TIGRFAMs: SSF46785;SSF52540

In one protein complex with GCW_03410 824955-825876(-)

>nucleotide sequence
CTAATTATCTTTAAAATATTTGATTGCCTTTTGTGTTATCTGCCGACCCTTTGAGGTTTT
CTTAATCAAGTTCATTTTGAGTAAATAAGGCTCGATCTTTGATTCAATTGTAAAACAATC
AATTCCACTTATTAGTGAAAGTGATTTCAAACCAAGATAAAGTTTGGGATTATCATCAAA
CGATTTAAGGTATTCCAGATCTAGATTTGATAAACCAAACTGATAGATATTTAATTTTTC
AAGGATTGATTTAATCGTAATCTCTTGATCAAATAACTTAAAATCCATCACCCGTTTATA
AATTCTTAAAGCATTGCGTGGTGTTCCTTTAGAATGTTCTGCTATCTGCACCATCTCATC
CTTGGTTAACTTAATCTTTTCTTTAACATTAATTGATCTGATGATCTCAAAGATCTCATC
ATCTTGATAATAATCAAGTTGTAATAAGATTCCAAACCGCTCTTCTAACGGATCGATGAT
CCTGCCATACATTGTCGTTGCACCAATTAATGTGAACGGTGGTAATTTCATTCTAGTAAA
TTTAGAATTAAATTCTTTACCGATCAAAATATCAACAACGTAATCTTCCATAATTGGAAA
TAATAGTTCCATAATTGTGGGTGATAACGAATGGATTTCATCAATAAACAAAACGTCGTT
TTTCGATAGTAACGAAAAAGCATTTAGTAGATCTGCAGGTTTTTGAATTTGCGATGCTTG
CAAGATCTTGATTTTTGATTTTAATTCATTAGCAATAATCTGAGCCAACGATGTTTTGCC
AACACCGGGTGGTCCATAAAATAACACATGGTCTAATGCTTTATTCTGGCTAATTGAGGC
ATTAATAAAAGTTAATAACTTTTGTTTTAATTCATTCTTACCAATAAACTCATCGAAATT
ATTTGGTCTAGCTAACTTCAC

>protein sequence
VKLARPNNFDEFIGKNELKQKLLTFINASISQNKALDHVLFYGPPGVGKTSLAQIIANEL
KSKIKILQASQIQKPADLLNAFSLLSKNDVLFIDEIHSLSPTIMELLFPIMEDYVVDILI
GKEFNSKFTRMKLPPFTLIGATTMYGRIIDPLEERFGILLQLDYYQDDEIFEIIRSINVK
EKIKLTKDEMVQIAEHSKGTPRNALRIYKRVMDFKLFDQEITIKSILEKLNIYQFGLSNL
DLEYLKSFDDNPKLYLGLKSLSLISGIDCFTIESKIEPYLLKMNLIKKTSKGRQITQKAI
KYFKDN