GCW_03420

Uniprot: A0A0F6CL90
Description: aspartyl/glutamyl-tRNA amidotransferase subunitB
EC number: 6.3.5.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. {ECO:0000256|HAMAP-Rule:MF_00121, ECO:0000256|SAAS:SAAS00247554}.; CATALYTIC ACTIVITY: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. {ECO:0000256|HAMAP-Rule:MF_00121}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln). {ECO:0000256|HAMAP-Rule:MF_00121, ECO:0000256|SAAS:SAAS00387483}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gatB
Gene names (synonym):
Mass: 54,913
Subunit structure [CC]: SUBUNIT: Heterotrimer of A, B and C subunits. {ECO:0000256|HAMAP-Rule:MF_00121, ECO:0000256|SAAS:SAAS00247550}.
Gene ontology (GO): ATP binding [GO:0005524]; glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity [GO:0050567]; transferase activity [GO:0016740]; translation [GO:0006412]
Gene ontology IDs: GO:0005524; GO:0006412; GO:0016740; GO:0050567
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GatB/GatE family. GatB subfamily. {ECO:0000256|HAMAP-Rule:MF_00121, ECO:0000256|SAAS:SAAS00548356}.
Protein families: GatB/GatE family, GatB subfamily
Coiled coil:
Domain [FT]: DOMAIN 322 470 GatB_Yqey. {ECO:0000259|SMART:SM00845}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99862
UniPathway: K02434
CDD:
Gene3D:
HAMAP:
InterPro: MF_00121
PANTHER: IPR004413;IPR017959;IPR006075;IPR018027;IPR003789;IPR017958
PIRSF: PTHR11659
PRINTS:
PROSITE:
Pfam: PS01234
ProDom: PF02934;PF02637
SMART:
SUPFAM: SM00845
TIGRFAMs: SSF89095

In one protein complex with GCW_03430 GCW_03425 828317-829742(-)

>nucleotide sequence
CTATCTATGTTTTTGAATTAACTCGGTTAATACTTTAATACTAACATTTGGGTTGGCTTG
TCCGTTGGTTTTTTTCATCAATAAACCAATTAATAATTTTTCCACCCGTTCTTTTCGTTG
ATCATAATCTTTTAAGATCGCAAGATTCTCAAGCACGATTGGTTCTAATAACGCAATGAT
CTCTTGTTCATCTTTGATCTGTTTCATCTTTAAGCGATCTACAATCTCTGCAACCGTTTC
ATCAGTTTTAATAATCTCATCTAAAACCACTTTAGCTTGTTTTTGGTTTAAATCTTCACC
TTTAATCAAAACTAAAAGGTCAGTTAAATTATCAAACCATTTGGGATTAATCAGTTCTAA
TTGCAACTTTTGCTTATTAATTCGACCCATAAATTCGATTAATAATCATTTACTAACAAA
AGCTAGATCATTAATTCTTTGATTAATCTCATTAATCTTTTGGTATAAAAAGAAATTGTC
TAATAGCAGATGAACAAACTGTTGATCTACTCCTTCAGATAACAGTTTGCTTTCTAACTC
TTTAATATTAAAAACTTGGATCTGATCAACTTCAGATAAGAACTGTTGATCAAGATTAAT
CCCAATAATGTTTGGTTCGAACATATAGCGATAATCAATCGCATTAGTTTTCTCACGCAT
GAAAACTGTTTGGTTTAATTGATCATCATAACGCATCGTTGCTTGCACAACTTTTTTGTT
TGACAATAACAACTCTTGTTGACGTGAGATCTCAAAATTAATTGCTTTTTCAACATTGTT
GATTGAATTAATGTTTTTGATTTCAACTCTGGTTCCATAGTTTGGTGCACCCATTGGTCG
AATTGAAACATTAACATCAACACGCATCGAACCTTCTTCTAATTTAGCATCACTAATATC
ATTAAAGATTAAGATTCTTCTTAGATTGGTGAGATATTCTTTAACTTCTTTCCCACTATC
AAAAACTGGATTAGTAACGATCTCAATTAATGGTGAACCAGCACGGTTGTAATCTAATAA
GATCTGGTTATCTTCATTCAGTTGTTTAGCCGTATCTTCTTCAATATGAAAACGTTGGAT
CGGAACTCTTTTATTTGAGATATCAAGATACCCATTAGTAGCAATCGGGTGGTGCTGTTG
GGTAATCTGATAACCTTTAGGTAGATCTTGATAAAAGTAGTTCTTTCGATCAAACCGTAG
GTGATGATCAACGTTTTGCATATTTAAAGCTTTAGCTAACACTAAAGCTTTCTTTACAAC
AAACCCATTTGGTTCAGGCATCGTTCCGGGTAAACCTAGATCAATGGGGTGAACGTTAAT
ATTAGGATCTGATCTGTGGGAGTTTGCTGATGGTGAAAACATTTTAGTTTTGGAATTGAT
CGCAACATGAACTTCAATTCCAATGATTGGTTGAAAATTATGCAT

>protein sequence
MHNFQPIIGIEVHVAINSKTKMFSPSANSHRSDPNINVHPIDLGLPGTMPEPNGFVVKKA
LVLAKALNMQNVDHHLRFDRKNYFYQDLPKGYQITQQHHPIATNGYLDISNKRVPIQRFH
IEEDTAKQLNEDNQILLDYNRAGSPLIEIVTNPVFDSGKEVKEYLTNLRRILIFNDISDA
KLEEGSMRVDVNVSIRPMGAPNYGTRVEIKNINSINNVEKAINFEISRQQELLLSNKKVV
QATMRYDDQLNQTVFMREKTNAIDYRYMFEPNIIGINLDQQFLSEVDQIQVFNIKELESK
LLSEGVDQQFVHLLLDNFFLYQKINEINQRINDLAFVSKWLLIEFMGRINKQKLQLELIN
PKWFDNLTDLLVLIKGEDLNQKQAKVVLDEIIKTDETVAEIVDRLKMKQIKDEQEIIALL
EPIVLENLAILKDYDQRKERVEKLLIGLLMKKTNGQANPNVSIKVLTELIQKHR