GCW_03550

Uniprot: A0A0F6CLB5
Description: translation initiation factor IF-1
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex. {ECO:0000256|HAMAP-Rule:MF_00075, ECO:0000256|SAAS:SAAS00571772}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): infA
Gene names (synonym):
Mass: 8,060
Subunit structure [CC]: SUBUNIT: Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly. {ECO:0000256|HAMAP-Rule:MF_00075, ECO:0000256|SAAS:SAAS00326798}.
Gene ontology (GO): cytoplasm [GO:0005737]; ribosome binding [GO:0043022]; rRNA binding [GO:0019843]; translation initiation factor activity [GO:0003743]
Gene ontology IDs: GO:0003743; GO:0005737; GO:0019843; GO:0043022
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IF-1 family. {ECO:0000256|HAMAP-Rule:MF_00075, ECO:0000256|SAAS:SAAS00571750}.; SIMILARITY: Contains 1 S1-like domain. {ECO:0000256|HAMAP-Rule:MF_00075, ECO:0000256|RuleBase:RU004366}.
Protein families: IF-1 family
Coiled coil:
Domain [FT]: DOMAIN 1 70 S1-like. {ECO:0000259|PROSITE:PS50832}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal: A0A0F6CLB5
MEROPS:
EnsemblBacteria KO: AHB99887
UniPathway: K02518
CDD:
Gene3D:
HAMAP: 2.40.50.140
InterPro: MF_00075
PANTHER: IPR012340;IPR006196;IPR004368
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50832
ProDom: PF01176
SMART:
SUPFAM:
TIGRFAMs: SSF50249
850113-850326(-)

>nucleotide sequence
TTAATGACGATAAATAATTCGCCCTTTTGTTAGATCATAAGGGCTGAATTCTACTTCCAC
ACTATCACCAGGTAAGATGCGGATCTTGTGCATCCGAATCTTTCCTGATACGTGTGCTTC
AATTAAAAGATTATTTTCTAGCAATACTTTAAATTTATCACCTTTAATAATCTCTTTCAC
AACACCTTTAATACTGAGGTTTGTTTCTTTCAT

>protein sequence
MKETNLSIKGVVKEIIKGDKFKVLLENNLLIEAHVSGKIRMHKIRILPGDSVEVEFSPYD
LTKGRIIYRH