GCW_03580

Uniprot: A0A0F6CLC0
Description: ribosome biogenesis GTPase RsgA
EC number: 3.6.1.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_01820}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01820}
Enzyme regulation:
Function [CC]: FUNCTION: May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00535149}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 238 238 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 243 243 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 245 245 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 252 252 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.
Nucleotide binding: NP_BIND 108 111 GTP. {ECO:0000256|HAMAP-Rule:MF_01820}.; NP_BIND 158 166 GTP. {ECO:0000256|HAMAP-Rule:MF_01820}.
Site:
Gene names (primary): rsgA
Gene names (synonym):
Mass: 31,677
Subunit structure [CC]: SUBUNIT: Monomer. Associates with ribosomes. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00151859}.
Gene ontology (GO): GTP binding [GO:0005525]; GTPase activity [GO:0003924]; metal ion binding [GO:0046872]
Gene ontology IDs: GO:0003924; GO:0005525; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00535129}.; SIMILARITY: Contains CP-type G (guanine nucleotide-binding) domain. {ECO:0000256|SAAS:SAAS00064598}.
Protein families: TRAFAC class YlqF/YawG GTPase family, RsgA subfamily
Coiled coil:
Domain [FT]: DOMAIN 58 215 CP-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51721}.; DOMAIN 67 213 EngC GTPase. {ECO:0000259|PROSITE:PS50936}.
Motif: MOTIF 238 252 Knuckle-like cysteine cluster. {ECO:0000256|HAMAP-Rule:MF_01820}.
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99892
UniPathway: K06949
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_01820
PANTHER: IPR030378;IPR027417;IPR004881;IPR010914
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50936;PS51721
ProDom: PF03193
SMART:
SUPFAM:
TIGRFAMs: SSF52540
857042-857867(-)

>nucleotide sequence
CTAAATTATGGACTTGATCACACTTAAATATATTTGATAGATTAATGAATTAATCTTTTT
CTTAAGATAACAATCAGCACTATTTTCAAGGTGTAAACAGTCGTTAAATTTACATTTAGT
TTGGTATTGAGCAAACTCAATTAAGCCACAACTTAGTTGTTGTTTAGTTAGGTTTAAGTC
ATAGATGGAAAAACCTGGAGAATCAACAAGATAACCGTCTAAAAACTTATAAAGTTTTGT
TGCAGTTGTCGTGTTTTTGCCTCGTTTTAGTTTTGTTGAAACGGCTTGAGTTCAGATATC
AAGCGAGTTATCTAACCGTTTAAGTAAGGTAGATTTACCCACCCCACTATGACCACAAAA
GATTGTTACCTTATTAGATAATAAGTTTTTTAAGCGCAAAAAATCATCTGATTCATTTAA
CTTGAACACTTGATAGTTTATCTGTTTTAAGGCCTCGACAATCTTTTTAAATTCGTTTTG
ATCGTGATCTAAATCTAGTTTAGAGAACACGATCAGTGGTTTGAATCCCAATTGTGATTC
AAAATAAACCATGAACTTAAACAGTTGTTTTAAATTTAGTTGGGGATCTTTAATTGAAAA
AACTAAAACAACATGATCAAGGTTGGCCACCTTAGGTCGGTTTAAGTAGTTCTTACGTTC
AATTAACTTAATGATCTCACCTTGATCATTAAGTTCTAATAGATCATTAGGTGCTAAATT
GATCTTTTGGTGTTTTCATTTTCCCGGAGCGTGAAGTTTTTTTAGTTCATGATTAACTTC
ACAAAAAAGATCATTAGCATTAATACTTAAAATTCTTGCAATCAT

>protein sequence
MIARILSINANDLFCEVNHELKKLHAPGKWKHQKINLAPNDLLELNDQGEIIKLIERKNY
LNRPKVANLDHVVLVFSIKDPQLNLKQLFKFMVYFESQLGFKPLIVFSKLDLDHDQNEFK
KIVEALKQINYQVFKLNESDDFLRLKNLLSNKVTIFCGHSGVGKSTLLKRLDNSLDIWTQ
AVSTKLKRGKNTTTATKLYKFLDGYLVDSPGFSIYDLNLTKQQLSCGLIEFAQYQTKCKF
NDCLHLENSADCYLKKKINSLIYQIYLSVIKSII