GCW_03610

Uniprot: A0A0F6CLC6
Description: tRNA uridine 5-carboxymethylaminomethylmodification protein
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000256|HAMAP-Rule:MF_00129, ECO:0000256|SAAS:SAAS00313811};
Enzyme regulation:
Function [CC]: FUNCTION: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000256|HAMAP-Rule:MF_00129}.
Pathway:
Active site:
Binding site: BINDING 120 120 FAD; via amide nitrogen and carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00129}.; BINDING 175 175 FAD. {ECO:0000256|HAMAP-Rule:MF_00129}.; BINDING 365 365 FAD. {ECO:0000256|HAMAP-Rule:MF_00129}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 8 13 FAD. {ECO:0000256|HAMAP-Rule:MF_00129}.; NP_BIND 268 282 NAD. {ECO:0000256|HAMAP-Rule:MF_00129}.
Site:
Gene names (primary): gidA
Gene names (synonym): mnmG
Mass: 68,867
Subunit structure [CC]: SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits. {ECO:0000256|HAMAP-Rule:MF_00129}.
Gene ontology (GO): cytoplasm [GO:0005737]; flavin adenine dinucleotide binding [GO:0050660]; oxidoreductase activity [GO:0016491]; tRNA wobble uridine modification [GO:0002098]
Gene ontology IDs: GO:0002098; GO:0005737; GO:0016491; GO:0050660
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the MnmG family. {ECO:0000256|HAMAP-Rule:MF_00129, ECO:0000256|SAAS:SAAS00537299}.
Protein families: MnmG family
Coiled coil:
Domain [FT]: DOMAIN 396 603 GIDA_assoc. {ECO:0000259|Pfam:PF13932}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99898
UniPathway: K03495
CDD:
Gene3D:
HAMAP: 3.50.50.60
InterPro: MF_00129
PANTHER: IPR023753;IPR026904;IPR004416;IPR002218;IPR020595
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01280;PS01281
ProDom: PF01134;PF13932
SMART:
SUPFAM:
TIGRFAMs: SSF51905
863137-864973(+)

>nucleotide sequence
ATGAATAAATTTATTGTAGTAGGAGCAGGCCATGCTGGCTTAGAAGCTGCGTTCATACTT
TCAAAGCTAAATAATAAGGTTTATCTTTGTGTTTTGGATAGAAAATACGTAGCTAACTGT
CCATGTAATCCTTCTGTTGGAGGACCAGCTAAAGGGATTGTTACAAGAGAAATAGACGCG
CTAGGTGGAATCCAAGCATTAGCTGCTGATAGCACTGCTCTTCAAAGAAAGATCTTAAAT
TCTTCAAAAGGACCTGGAGTTCAATGTTTAAGATTTCAAATCGATAAAGTTTATTACAAG
AAATGATTCTTAGAACAAATTGATAATAATGAAAATATTGAACTAGTTGAAGGTGAAGTA
ACAGAAGTAATTAAGAACGGTGATACTGCTACTGGAGTAATGATTGATGGCGCAAAAAAA
TTAGAGGCTGACGCAGTAATAATTACTACAGGTACTTATTTAAAATCATTGACATTTAGC
GGTAAAGACGTAAAAAATGAAGGTCCAGAAGGTTTTAAGAACTCTAATAATTTATCTGAA
TGATTTAAAGTAAATGGGTTTGAATTAACTAGATTAAAAACAGGTACCCCTCCTAGGATT
AAAAAAGATTCAATTGATTATTCAAATTTACAAATAGAGCCAGGAAATGGAACAGAGTTA
TATTTTTCTCACTGGTCTAAAAATAAATACATCGATTATGAATTGCCTTGTTATTTAATC
CATACAACAGAAGAAATTCATAAGATAATCAACGATAATTTACATTTATCAGCGATGTAT
TCAGGAAATATCACGGGTGTAGGTCCTAGATATTGTCCTAGTATTGAAGATAAGATTGTT
AGATTCTCTAATAAGCCAAGACATCAAATTTTTCTTGAACCGGAATCTTTAGAACTTGAT
ACTGTTTATTTAGGTGGTTTTTCAACTTCTTTAGATATCTCTGTTCAAGATAAAATTATT
CGACTTTTACCTGGGCTAAAAAAAGCTGAAGTTATCAAATACGGATATGCAATTGAATAT
GATGCAATTAATCCAATACAACTATACCCTTCTTTAGAATCTAAATTAGTAAAAAATCTA
TTTTTTGCCGGACAAATTAACGGAACTAGTGGTTATGAAGAAGCTGCGGGACAAGGTTTA
ATTGCTGGCATAAATGCAAACCAAAAAATTAAAAATAAAGAGCCTTTAATCTTATCTAGA
AATGAAGCATACATTGGGGTAATGATTGACGATATTGTAACTAAAGGAGTAACAGATCCT
TATCGTTTATTAACATCTCGAGCTGAATATAGATTATTGTTAAGAAATGATAATGTTTTA
GATCGATTAATACAAAAAGGTTATGAAATCGGCACAATATCTAAGGAACAAATAGATTTA
TATAAGCAAAACTTAGAAAAGAAAAATAAACTTATTGAATTTTTAAAGGATAAAAAGGTA
GGTATGTATACATTATTAAGAGCACATACAAACAATACAAATTTCTCACTTTATGAATTC
TTAAAACGACCAGAAATTAAATTAATTGAACTGTTAAAATTAATTGAATTTGATTATAGT
AATTACGATCTAGAACTATTAAAAAACATCGAAATTACCGTAAAATATGAAGGTTATATT
AAAAAGGAGAGTAGAATTGTTAACTCTTTAAAGAACTTGGAAAGTATTAAAATTCCTCAA
GATCTTATTTATGATAAAGTGCAAAATCTTTCAATTGAAGCAATCGATAAGTTAAACAAA
ATAAAGCCTCTTAATTTAGCACAGGCTCAGCGTATTAGTGGAATCAATTTAGCTGACATT
ATTTCTTTGAAAACTCATTTAGAACAAAATGCTTAA

>protein sequence
MNKFIVVGAGHAGLEAAFILSKLNNKVYLCVLDRKYVANCPCNPSVGGPAKGIVTREIDA
LGGIQALAADSTALQRKILNSSKGPGVQCLRFQIDKVYYKKWFLEQIDNNENIELVEGEV
TEVIKNGDTATGVMIDGAKKLEADAVIITTGTYLKSLTFSGKDVKNEGPEGFKNSNNLSE
WFKVNGFELTRLKTGTPPRIKKDSIDYSNLQIEPGNGTELYFSHWSKNKYIDYELPCYLI
HTTEEIHKIINDNLHLSAMYSGNITGVGPRYCPSIEDKIVRFSNKPRHQIFLEPESLELD
TVYLGGFSTSLDISVQDKIIRLLPGLKKAEVIKYGYAIEYDAINPIQLYPSLESKLVKNL
FFAGQINGTSGYEEAAGQGLIAGINANQKIKNKEPLILSRNEAYIGVMIDDIVTKGVTDP
YRLLTSRAEYRLLLRNDNVLDRLIQKGYEIGTISKEQIDLYKQNLEKKNKLIEFLKDKKV
GMYTLLRAHTNNTNFSLYEFLKRPEIKLIELLKLIEFDYSNYDLELLKNIEITVKYEGYI
KKESRIVNSLKNLESIKIPQDLIYDKVQNLSIEAIDKLNKIKPLNLAQAQRISGINLADI
ISLKTHLEQNA