GCW_03710

Uniprot: A0A0F6CLE4
Description: thymidine kinase
EC number: 2.7.1.21
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5'-phosphate. {ECO:0000256|HAMAP-Rule:MF_00124, ECO:0000256|RuleBase:RU000544}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site: ACT_SITE 94 94 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00124}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 150 150 Zinc. {ECO:0000256|HAMAP-Rule:MF_00124}.; METAL 153 153 Zinc. {ECO:0000256|HAMAP-Rule:MF_00124}.; METAL 188 188 Zinc. {ECO:0000256|HAMAP-Rule:MF_00124}.; METAL 191 191 Zinc. {ECO:0000256|HAMAP-Rule:MF_00124}.
Nucleotide binding: NP_BIND 19 26 ATP. {ECO:0000256|HAMAP-Rule:MF_00124}.; NP_BIND 93 96 ATP. {ECO:0000256|HAMAP-Rule:MF_00124}.
Site:
Gene names (primary): tdk
Gene names (synonym):
Mass: 25,259
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00124}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; thymidine kinase activity [GO:0004797]; zinc ion binding [GO:0008270]; DNA biosynthetic process [GO:0071897]
Gene ontology IDs: GO:0004797; GO:0005524; GO:0005737; GO:0008270; GO:0071897
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000256|HAMAP-Rule:MF_00124, ECO:0000256|RuleBase:RU004165}.
Protein families: Thymidine kinase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal: A0A0F6CLE4
MEROPS:
EnsemblBacteria KO: AHB99916
UniPathway: K00857
CDD:
Gene3D:
HAMAP:
InterPro: MF_00124
PANTHER: IPR027417;IPR001267;IPR020633
PIRSF: PTHR11441
PRINTS: PIRSF035805
PROSITE:
Pfam: PS00603
ProDom: PF00265
SMART:
SUPFAM:
TIGRFAMs: SSF52540
885171-885846(+)

>nucleotide sequence
ATGGCTAAGAAGAACGCAATGACCACCACTAATGGCTGAATCGAAGCAATTTGTGGTCCG
ATGTTTGCTGGTAAAACAGATGAACTAATTCGCAAGATTAAACGTTATGAGTATGCTGAT
GTAAAGTCATTGGTCTTTTCACCAGCAACTGATACTCGTTCAGCTCAAGAGATTATCAAC
TCACGAGATGGTCGTAGAATTGGTTCAATAAAGATCAAAAAAGCATTTGAAATCTATGAT
TATGTTTTGTTACATAAACCACAATTAGTTGGAATTGATGAAGTACAATTCTTTGATGAT
TCAATTGTTGAAGTCATCCAAACATTAGCTGATAACCAAATTAACGTAATTGTGGCTGGT
TTAGACCGCGATTTTAGAGGTGAACCTTTTGGTCCAATTCCTAAAATCTTAGGAATTGCA
GAATCAGTAATCCGCTTAACCGCAATCTGTTCTGAATGTGGTGCTGAAGCTAGTCGAAGT
CAACGTTTAATTGATAACCAACCAGCTGACTATAATTGCGAAACGATTCTTATTGGTGAC
ACTGAATCATACGCGCCAAGATGTCGTCATCACCATAAAGTGCCTAACCGGCCAATTAAC
GACCAAACCAAGAACTTCAAACGTGCACTAAAAAATAATTTTGATAAAATAGTAGAGCAA
TCTTCAAAAGATTAA

>protein sequence
MAKKNAMTTTNGWIEAICGPMFAGKTDELIRKIKRYEYADVKSLVFSPATDTRSAQEIIN
SRDGRRIGSIKIKKAFEIYDYVLLHKPQLVGIDEVQFFDDSIVEVIQTLADNQINVIVAG
LDRDFRGEPFGPIPKILGIAESVIRLTAICSECGAEASRSQRLIDNQPADYNCETILIGD
TESYAPRCRHHHKVPNRPINDQTKNFKRALKNNFDKIVEQSSKD