GCW_03765

Uniprot: A0A0F6CLF3
Description: CRISPR-associated protein Cas1
EC number: 3.1.-.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01470}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|HAMAP-Rule:MF_01470};
Enzyme regulation:
Function [CC]: FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette. {ECO:0000256|HAMAP-Rule:MF_01470}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 166 166 Manganese or magnesium. {ECO:0000256|HAMAP-Rule:MF_01470}.; METAL 223 223 Manganese or magnesium. {ECO:0000256|HAMAP-Rule:MF_01470}.; METAL 238 238 Manganese or magnesium. {ECO:0000256|HAMAP-Rule:MF_01470}.
Nucleotide binding:
Site:
Gene names (primary): cas1
Gene names (synonym):
Mass: 36,094
Subunit structure [CC]: SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer. {ECO:0000256|HAMAP-Rule:MF_01470}.
Gene ontology (GO): DNA binding [GO:0003677]; endonuclease activity [GO:0004519]; metal ion binding [GO:0046872]; defense response to virus [GO:0051607]; maintenance of CRISPR repeat elements [GO:0043571]
Gene ontology IDs: GO:0003677; GO:0004519; GO:0043571; GO:0046872; GO:0051607
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family. {ECO:0000256|HAMAP-Rule:MF_01470}.
Protein families: CRISPR-associated endonuclease Cas1 family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99925
UniPathway: K15342
CDD:
Gene3D:
HAMAP:
InterPro: MF_01470
PANTHER: IPR002729;IPR019855
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01867
SMART:
SUPFAM:
TIGRFAMs:
901093-902020(+)

>nucleotide sequence
ATGCAGCTGCCGCATCCGCCAGAGCATTTATGCTCTGGCTTTTTATTTGTTATGTCTAAA
AAAGTCCTTGAAGTTTCTGAAACTGAATTTATTAGCTTACATCTAGACACGTTAATTGTT
AGAAAACACGGAGCTAGAGTCACCCTACCACTAAAAACTTTAGATACTATTCTTATTACC
AATCCGTATTGCAACATATCAGTTCCTCTATTAAATAAAATTGTTGAAAACAATATTAAT
CTAATTGTGTGTAATACTAAGTTTCAACCAACGATGCAAATCTTACCAATTAGCTCGTAT
TATTCAAATAAGAATTATTTGTGGCAAATCAGATGAACTGATGAACAGAAGAATCAGATT
TGAAAACAGCTTGTAAAATTAAAGATTATTAATTATGCAAGGTTGATTAATCATCTGAAT
ATTACAACCGATGATAATATCGAAAAACTAACCAATTATTACACCAATGTGGTTGATGGC
GATTTAAACAACTGTGAAGGTCACGCAGCCAAGCTTTCGTTCAAACTGTTATACGGTGAA
GATTTTAGTCGTTCGCAAAAAGACGATATCATTAATAAATATCTAAATTATGGTTATATT
GTTTTAATGACTTATGTTTCACGAACATTGGTTAAGAATGGCTTAGATAATAGAATTGGG
ATTTTTCATAAGAGCTTTAACAATCATTTTGCATTGTCATGTGACATTATGGAGCCTTTT
AGACCAATTATTGATTATCTAACCTTTTCTTATTTGATTAAAAATCAAAACGATGATTTC
AAATCATATAAAAAAGATCTATTTATATCTTTTGAGAACTTTATCTTCCCAAATGGTAAA
AACCTGAACCAAGTTATCGATATGTTAGTTAAGGCAATTATCAATAACAAAATCAACGAA
AGGGATTTTAATATTGATTGAAGTTAA

>protein sequence
MQLPHPPEHLCSGFLFVMSKKVLEVSETEFISLHLDTLIVRKHGARVTLPLKTLDTILIT
NPYCNISVPLLNKIVENNINLIVCNTKFQPTMQILPISSYYSNKNYLWQIRWTDEQKNQI
WKQLVKLKIINYARLINHLNITTDDNIEKLTNYYTNVVDGDLNNCEGHAAKLSFKLLYGE
DFSRSQKDDIINKYLNYGYIVLMTYVSRTLVKNGLDNRIGIFHKSFNNHFALSCDIMEPF
RPIIDYLTFSYLIKNQNDDFKSYKKDLFISFENFIFPNGKNLNQVIDMLVKAIINNKINE
RDFNIDWS