GCW_03770

Uniprot: A0A0F6CLF4
Description: CRISPR-associated protein Cas2
EC number: 3.1.-.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01471};
Enzyme regulation:
Function [CC]: FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette. {ECO:0000256|HAMAP-Rule:MF_01471}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 16 16 Magnesium; catalytic. {ECO:0000256|HAMAP-Rule:MF_01471}.
Nucleotide binding:
Site:
Gene names (primary): cas2
Gene names (synonym):
Mass: 13,182
Subunit structure [CC]: SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer. {ECO:0000256|HAMAP-Rule:MF_01471}.
Gene ontology (GO): endoribonuclease activity [GO:0004521]; metal ion binding [GO:0046872]; defense response to virus [GO:0051607]; maintenance of CRISPR repeat elements [GO:0043571]
Gene ontology IDs: GO:0004521; GO:0043571; GO:0046872; GO:0051607
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2 protein family. {ECO:0000256|HAMAP-Rule:MF_01471}.
Protein families: CRISPR-associated endoribonuclease Cas2 protein family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99926
UniPathway: K09951
CDD:
Gene3D:
HAMAP: 3.30.70.240
InterPro: MF_01471
PANTHER: IPR021127;IPR000640;IPR019199
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF09827
SMART:
SUPFAM:
TIGRFAMs:
902006-902336(+)

>nucleotide sequence
TTGATTGAAGTTAATAGTAGGTATATGAGAATCGTAATTTTTTATGACATTTATGAAACT
GAAGAAGTTGATAAGCTTGCTAAAAAGTTTCGTGACAACTTAATCTCTTTAGGTTATTTT
ATGATCCAATATTCAGTATACTGTAAGTGTGTTGGTTCACACACCATGTATGATTACGAA
ATTAAAAAGATTCGTAAGATATTACCAGTAAAAGCGAATGTAAGAATCTTATTGTTATCT
GAAAAACAATACCAAAATATGCTAATCATAAGTGGTGAGAAAAATCTTAACGAAATTTAC
AACGATCGAGAGGATTACATAGAAATCTAA

>protein sequence
LIEVNSRYMRIVIFYDIYETEEVDKLAKKFRDNLISLGYFMIQYSVYCKCVGSHTMYDYE
IKKIRKILPVKANVRILLLSEKQYQNMLIISGEKNLNEIYNDREDYIEI