GCW_04010

Uniprot: A0A0F6CLI6
Description: glutamyl-tRNA synthetase
EC number: 6.1.1.17
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). {ECO:0000256|HAMAP-Rule:MF_00022}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). {ECO:0000256|HAMAP-Rule:MF_00022}.
Pathway:
Active site:
Binding site: BINDING 255 255 ATP. {ECO:0000256|HAMAP-Rule:MF_00022}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gltX
Gene names (synonym):
Mass: 57,874
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00022}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; glutamate-tRNA ligase activity [GO:0004818]; tRNA binding [GO:0000049]; glutamyl-tRNA aminoacylation [GO:0006424]
Gene ontology IDs: GO:0000049; GO:0004818; GO:0005524; GO:0005737; GO:0006424
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00022, ECO:0000256|RuleBase:RU363037}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 3 323 tRNA-synt_1c. {ECO:0000259|Pfam:PF00749}.
Motif: MOTIF 10 20 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_00022}.; MOTIF 252 256 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_00022}.
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99958
UniPathway: K01885
CDD:
Gene3D:
HAMAP: 1.10.10.350;1.10.1160.10;3.40.50.620
InterPro: MF_00022_B
PANTHER: IPR008925;IPR020751;IPR001412;IPR004527;IPR000924;IPR020061;IPR020058;IPR014729
PIRSF:
PRINTS:
PROSITE: PR00987
Pfam: PS00178
ProDom: PF00749
SMART:
SUPFAM:
TIGRFAMs: SSF48163
968006-969503(-)

>nucleotide sequence
TTACTTGGCATTAGTTAGATAATTGATGGCATCATCAAGATTTTTAAGAATTGTTTGTTT
ATCATACAAAGCAATCACTTTGTTTAACTCTGGACCGTGCTTTTTATTAGATAAAACAAT
TCTTAATGGCATATATAAGTTCTTACCACCTAAAGGTTTTTCTAAAACCAAATCACCTAG
TTTAATTTGGTGAGCTAACCAATTAATAATCTCTTTAACGTTATGGTCATTAACCTGATC
AAGTTGATCAATCTTCTCTTTAAATGAGATTAAAAGTTGACGATAATTTGGATTAGATTT
TAAGAAATCTAGATCAGCTTGATCAAGGTTAGATAACCCAACATGGGGAACAAAACTTTC
TTTAATGATCTCGTTGATCTGATAACCATACTGTAATTGGTTTTTAAACATCAAGCAGAT
CTCATTAACTTTAGTTTTAATCTCATCATATTCATTAAGATCAACATATGGTTTGATGAA
TGCTAAATACATAACATCATCCATATTTTTGATGTATTCAGATGAGATTCAATTTAGTTT
TTTAATGTCAAAAAAAGAAGGGGCTGCACTTAAGTTTTTAATCGTAAACGATTTAACCAA
CGTAGGTAAATCTAAGATCTCAATATTATCTGGGTGAGATCAACCTAATAAAGCAATAAA
GTTAACTAGTGCATCTGCTAAATAACCTTTTTTTCTAAACCCTTCAACGAACTGTTCAAC
CGCTAAATTTCTTTTAGATAATTTCTTACCAGTTTCATCAACAATGATTGATAGATGACC
ATAAACAAATTCATTTTTGAACCCTAAAGCTTCTTTGATCGCTAACTGATAGGGAGTATT
TGAGATATGCTCTTCTCCTCTTAAGATATGTGAGATCTTCATATCATGGTCATCGATCAC
AACCGCAAAGTTGTACGTCGCGATCTGATTTGATTTTAAGATCACAGGGTCGCTCATTGA
ACTAGTATTAAAGATTAAGTTCCCACGAATTAAATCGTTTCAAGAATATTCCTTATTTTC
TTTTAATAATAATCTGATCGTAAAAGGAATCTTATCAGCCAAATTTTTTTGAATCTGCTC
TTCTGAAAGAAGAAAACACTTTCTTGAATAAATTGGTGGTTTATACTGTTTTAATAAGTC
TCCACGGTGTTTTTGTAGTTCTTCAGGTGTACAAAAACAACGGTATGCTTTTTTTTCTTC
TAAAAGTTGGTAGGCATATTTTTGGTAAACTTCTAGTTTTTCAGACTGACGATAAGGTCC
GCTTCGAGTCGGGTTTCAAATTGATTCGTCAGCAAAAATGTTTAATCACTTTAGGTTATA
AAGTTGGTTCTCAGCACCATCTTCAACGTTTCTTTCAATGTCCGTATCTTCGATACGAAC
AATAAATTCCCCGTTGTTGTGCTTGGCAAATAAATAATTAAATAAAGCTGTGCGTGCACC
GCCAATGTGGAAATGCCCTGTTGGCGATGGTGCGTATCTGGTTCTAATCTTAGACAT

>protein sequence
MSKIRTRYAPSPTGHFHIGGARTALFNYLFAKHNNGEFIVRIEDTDIERNVEDGAENQLY
NLKWLNIFADESIWNPTRSGPYRQSEKLEVYQKYAYQLLEEKKAYRCFCTPEELQKHRGD
LLKQYKPPIYSRKCFLLSEEQIQKNLADKIPFTIRLLLKENKEYSWNDLIRGNLIFNTSS
MSDPVILKSNQIATYNFAVVIDDHDMKISHILRGEEHISNTPYQLAIKEALGFKNEFVYG
HLSIIVDETGKKLSKRNLAVEQFVEGFRKKGYLADALVNFIALLGWSHPDNIEILDLPTL
VKSFTIKNLSAAPSFFDIKKLNWISSEYIKNMDDVMYLAFIKPYVDLNEYDEIKTKVNEI
CLMFKNQLQYGYQINEIIKESFVPHVGLSNLDQADLDFLKSNPNYRQLLISFKEKIDQLD
QVNDHNVKEIINWLAHQIKLGDLVLEKPLGGKNLYMPLRIVLSNKKHGPELNKVIALYDK
QTILKNLDDAINYLTNAK