GCW_04015

Uniprot: A0A0F6CLI7
Description: methenyltetrahydrofolate cyclohydrolase
EC number:
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_01576}.; CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH. {ECO:0000256|HAMAP-Rule:MF_01576}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_01576}.
Pathway: PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. {ECO:0000256|HAMAP-Rule:MF_01576}.
Active site:
Binding site: BINDING 224 224 NADP; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_01576}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 157 159 NADP. {ECO:0000256|HAMAP-Rule:MF_01576}.
Site:
Gene names (primary): folD
Gene names (synonym):
Mass: 32,223
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01576}.
Gene ontology (GO): methenyltetrahydrofolate cyclohydrolase activity [GO:0004477]; methylenetetrahydrofolate dehydrogenase (NADP+) activity [GO:0004488]; folic acid-containing compound biosynthetic process [GO:0009396]; histidine biosynthetic process [GO:0000105]; methionine biosynthetic process [GO:0009086]; purine nucleotide biosynthetic process [GO:0006164]; tetrahydrofolate interconversion [GO:0035999]
Gene ontology IDs: GO:0000105; GO:0004477; GO:0004488; GO:0006164; GO:0009086; GO:0009396; GO:0035999
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. {ECO:0000256|HAMAP-Rule:MF_01576}.
Protein families: Tetrahydrofolate dehydrogenase/cyclohydrolase family
Coiled coil:
Domain [FT]: DOMAIN 5 114 THF_DHG_CYH. {ECO:0000259|Pfam:PF00763}.; DOMAIN 125 277 THF_DHG_CYH_C. {ECO:0000259|Pfam:PF02882}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99959
UniPathway: K01491
CDD:
Gene3D:
HAMAP: 3.40.50.720
InterPro: MF_01576
PANTHER: IPR016040;IPR000672;IPR020630;IPR020631
PIRSF:
PRINTS:
PROSITE: PR00085
Pfam:
ProDom: PF00763;PF02882
SMART:
SUPFAM:
TIGRFAMs: SSF51735
969533-970385(-)

>nucleotide sequence
CTATTTATTAACCTTCTGAATTTCAGATAAGTTAATTAAGTTCTTGACTAAAGAATAAAT
CGTTAGTGGGCCAATCCCACCAGGTGTAGGCGTACCATATGAAGCAATCTCTTTGAGCTT
GTCATAATCAAAATCACCACAAACAAGGTATTTGGATTGTTCTGGATCAAAATATTTATC
GATCCCAATATCGATAAATATCACTCCTTGTTTGAACTCATAATTCCCCAAAAACTTCGC
CTTACCCACTGCTGAGATTACTAAATCTGCTACTTTAGTTTTTTCTTCTAACAGTTGACT
ATTTTCTTTTGTAATCAGATCAAAGTTGATCTGATGTTCATTTAAATAATTAACAATTGG
TTGGTTTGCTGTAATCCCATTACCAATCAATAAGATCTTTTTATCTAAAAAACTAAGCTG
GTATTCTTTAAATAGTTCTAGCACTGCATTAAGCACACAAGGGATAACTTTTTCTTTTTG
ATCTTGATCAAAACGGTGATACAAAAAAGCATCCACATCCAGATCAATGGGGATATTTTC
AATGATCTTATTTGTATCTAATCTTTCTTTGATTGGTAATTGAACCAATACTCCGTTGGG
ATTACTTAAACTAATCTTTTGATTCATCTTAACGATGAATTGGTTCGTATTATCAATCTT
AGATAGATCATAAACTTCAGTTTGAATCCCTAATGATTCGCAATAATTAATCTTATTGCG
AACATAGATTCTAGAAGCCTCTGAAGGATCACTAATGATGATTAATAATTTAATCTTTTG
GTTATTAACTTTTTTAGCTAAATCTTCTTTTAGTTTTTGTGATAATTTCGTGCCATCTAA
TTTAATAAACAT

>protein sequence
MFIKLDGTKLSQKLKEDLAKKVNNQKIKLLIIISDPSEASRIYVRNKINYCESLGIQTEV
YDLSKIDNTNQFIVKMNQKISLSNPNGVLVQLPIKERLDTNKIIENIPIDLDVDAFLYHR
FDQDQKEKVIPCVLNAVLELFKEYQLSFLDKKILLIGNGITANQPIVNYLNEHQINFDLI
TKENSQLLEEKTKVADLVISAVGKAKFLGNYEFKQGVIFIDIGIDKYFDPEQSKYLVCGD
FDYDKLKEIASYGTPTPGGIGPLTIYSLVKNLINLSEIQKVNK