GCW_04045

Uniprot: A0A0F6CLJ1
Description: tRNA modification GTPase TrmE
EC number: 3.6.-.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000256|HAMAP-Rule:MF_00379}; ; Note=Binds 1 potassium ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00379}
Enzyme regulation:
Function [CC]: FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000256|HAMAP-Rule:MF_00379}.
Pathway:
Active site:
Binding site: BINDING 28 28 Formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_00379}.; BINDING 86 86 Formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_00379}.; BINDING 125 125 Formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_00379}.; BINDING 453 453 Formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_00379}.
Calcium binding:
DNA binding:
Metal binding: METAL 231 231 Potassium. {ECO:0000256|HAMAP-Rule:MF_00379}.; METAL 235 235 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00379}.; METAL 250 250 Potassium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00379}.; METAL 252 252 Potassium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00379}.; METAL 255 255 Potassium. {ECO:0000256|HAMAP-Rule:MF_00379}.; METAL 256 256 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00379}.
Nucleotide binding: NP_BIND 231 236 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.; NP_BIND 250 256 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.; NP_BIND 276 279 GTP. {ECO:0000256|HAMAP-Rule:MF_00379}.
Site:
Gene names (primary): trmE
Gene names (synonym): mnmE
Mass: 51,666
Subunit structure [CC]: SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits. {ECO:0000256|HAMAP-Rule:MF_00379}.
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; metal ion binding [GO:0046872]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0003924; GO:0005525; GO:0005737; GO:0006400; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. {ECO:0000256|HAMAP-Rule:MF_00379, ECO:0000256|RuleBase:RU003313, ECO:0000256|SAAS:SAAS00534589}.; SIMILARITY: Contains TrmE-type G (guanine nucleotide-binding) domain. {ECO:0000256|SAAS:SAAS00027953}.
Protein families: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, TrmE GTPase family
Coiled coil: COILED 174 208 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 221 375 TrmE-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51709}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal: A0A0F6CLJ1
MEROPS:
EnsemblBacteria KO: AHB99963
UniPathway: K03650
CDD:
Gene3D:
HAMAP: 1.20.120.430;3.30.1360.120;3.40.50.300
InterPro: MF_00379
PANTHER: IPR031168;IPR018948;IPR006073;IPR004520;IPR027368;IPR025867;IPR027417;IPR005225;IPR027266
PIRSF:
PRINTS:
PROSITE: PR00326
Pfam: PS51709
ProDom: PF01926;PF12631;PF10396
SMART:
SUPFAM:
TIGRFAMs: SSF52540
974513-975875(-)

>nucleotide sequence
TTATTTACCTAGACAAAAGTTTTTAAAGATCTCATCGATCAGATTGTAGTCTTCGTATTC
AGATAATACTTTTAATAGTGAATTATTAGCTTCATTTAAGTGTTGAATAACAAGATCATG
ATATTGGTTAGGGTCGTTTAATATTAAATTAATATTGTACAGAGCTGTTTGTAATAGGTT
GATTTGTCATTCTTCTTGAAAGATACTATCTTGTTTATTTTCGTCATTAAAAATTGCTAA
ACTAGTTTCTTCTAAATATTTAATTAAGTCACTAATATCGTTATTTTTAGCACTGATCTT
AATTTGAATCTCACCAGTGTTTAAAGTGGGGTCTACTTCATCTACTTTGTTGCCAACTAA
AACAAACTGTTTTTTCTTATTAATTAAATATTTGTAAATGTTAGTTTTGTCGTAATTTTG
ATATTTATTTAAATCGATAAGGTAGATTACTAAATCTGATTTATCTATTGTTTTAAAACT
TTTGTTTATTCCGATTTGTTCAATTTGATCACTACTTTTTCTAATTCCAGCGGTGTCAAT
AAGATTAATTATTAATTCATCGTTTAAAACAATCTGACCTTCTACTGTATCTCTTGTGGT
TCCTGGGATATTGGTAACAATTGCTTTTTGTTCGTTTAATAATGCATTTAATAATGAAGA
TTTTCCTGCATTAGGTTCGCCAACGATAGCAATCTTTATTCCTTCAGAAATTTGTTTTAA
TTTTAACGATTTATTAACAATTCTTTGCAGTTTTTGCCGTATATTTGTTAAATTTTTAAA
TTCTTCAGTAAGTTCTTTTTTCTCGTCTTCGTACTCAGGATAATCAATAGCAATTTCTAC
TAAACCAATAAGTCTAAAAATTTCTTGTTGAATATTTTTGATGTCCTTAGAAAACTCACC
ACTTAAAGCTTTAATACTAGCAGAATGACTTAAGTTATTTTTTGCAAAAATTAGATCATG
AATTGCGGTAGCTTGATTAAGATCAATCTTTTTGTTTATATAACTTCGTTTAGAAAACTC
CCCTCTTCGAGCTAAATGTGCTCCGTGCTTGTTTAATAAGGCCATTATCTTATTAATAAC
AAAAAGCCCACCGTGACAATTAATTTCAATAGAATCTTCACCAGTAAAGCTTTTTGGTGC
CACAAACTTCATTAATAAGACATCATCGATTATCTGATCATTATCTACTATTCTTGCATT
CTGAATTCGATAACCTTCTTTAGTTATTTGTTTGTCACAAATTTTATTAATGATCTCAAA
AGCATCAGGGCCAGATAATCTAATCACATGAATCGCACTATTATACGGGGCTGTTGCTAA
AGCATAAATGGTTTCGTATTTTTTCATGATTTTATTAATCAA

>protein sequence
LINKIMKKYETIYALATAPYNSAIHVIRLSGPDAFEIINKICDKQITKEGYRIQNARIVD
NDQIIDDVLLMKFVAPKSFTGEDSIEINCHGGLFVINKIMALLNKHGAHLARRGEFSKRS
YINKKIDLNQATAIHDLIFAKNNLSHSASIKALSGEFSKDIKNIQQEIFRLIGLVEIAID
YPEYEDEKKELTEEFKNLTNIRQKLQRIVNKSLKLKQISEGIKIAIVGEPNAGKSSLLNA
LLNEQKAIVTNIPGTTRDTVEGQIVLNDELIINLIDTAGIRKSSDQIEQIGINKSFKTID
KSDLVIYLIDLNKYQNYDKTNIYKYLINKKKQFVLVGNKVDEVDPTLNTGEIQIKISAKN
NDISDLIKYLEETSLAIFNDENKQDSIFQEEWQINLLQTALYNINLILNDPNQYHDLVIQ
HLNEANNSLLKVLSEYEDYNLIDEIFKNFCLGK