GCW_04065

Uniprot: A0A0F6CLJ5
Description: DNA gyrase subunit A
EC number: 5.99.1.3
Annotation score: 3 out of 5
Miscellaneous [CC]: MISCELLANEOUS: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes. {ECO:0000256|HAMAP-Rule:MF_01897}.
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000256|HAMAP-Rule:MF_01897, ECO:0000256|SAAS:SAAS00075911}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. {ECO:0000256|HAMAP-Rule:MF_01897}.
Pathway:
Active site: ACT_SITE 132 132 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000256|HAMAP-Rule:MF_01897}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gyrA
Gene names (synonym):
Mass: 94,245
Subunit structure [CC]: SUBUNIT: Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis. {ECO:0000256|HAMAP-Rule:MF_01897}.
Gene ontology (GO): chromosome [GO:0005694]; cytoplasm [GO:0005737]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (ATP-hydrolyzing) activity [GO:0003918]; DNA topological change [GO:0006265]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0003677; GO:0003918; GO:0005524; GO:0005694; GO:0005737; GO:0006261; GO:0006265
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the topoisomerase GyrA/ParC subunit family. {ECO:0000256|HAMAP-Rule:MF_01897}.; SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. {ECO:0000256|SAAS:SAAS00565436}.
Protein families: Topoisomerase GyrA/ParC subunit family; Type II topoisomerase GyrA/ParC subunit family
Coiled coil: COILED 446 487 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 21 474 TOP4c. {ECO:0000259|SMART:SM00434}.
Motif: MOTIF 536 542 GyrA-box. {ECO:0000256|HAMAP-Rule:MF_01897}.
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHB99967
UniPathway: K02469
CDD:
Gene3D:
HAMAP: 3.30.1360.40;3.90.199.10
InterPro: MF_01897
PANTHER: IPR024946;IPR005743;IPR006691;IPR013760;IPR002205;IPR013758
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF03989;PF00521
SMART:
SUPFAM: SM00434
TIGRFAMs: SSF56719

In one protein complex with GCW_04070 978567-981108(-)

>nucleotide sequence
TTATTCTTTATCTTCATCTTGATTTGAATCATCTGATGATGATTCTGTATCAGGATTATC
TGAACCAACTTGTTGACTATCATCTTGTCCAACATCAAAGATTGCTACAGATTGTAGTGT
TTCTTTTTCATTTAATGAGATTAATTTAACACCAGAGGTGTTTCGTCCTTGTTCAGAAAC
CTCAGCTAATGGAACACGAACGATCTTACCTGTTGATGAAATCATTAATAGCTCTTCATT
ACCATTAACTAATTGCGTGTTAATGATATTTCCGGTTTTAGGTGTAACTTTGATCGTAAT
CACCCCTTTAGAACCACGGTTGGTCATGCGGTACTCTTCACGGTCAGTCATCTTCCCAAC
ACCTTTTTCACAAACCGCAAGTAATAAACTACCTTCATGGGATGATGATAATCCAATTAC
TTCATCATTAGGGTTTCTGAATTTAACACCAATTACCCCTTGAGCAGTTCGACCCATTGA
ACGCACAGCATCTTCTTTAAATCTTACAAGTAGACCTGATGAAACACCAATATAAATCTC
TTCGTCACCTGCAGTTTTAATTACCTTGAATAAACTGTCGTTTTCAGTTAACTTAATCGC
AATCTTTCCGTTGTTTTGAATTCTAGAAAACTCAGACGCTTTAACCCGTTTAACAAGACC
TTTTCTCGTTGAGAAGAAGAAATAACCGCTGTCATAATCATTGATTGATAATAATGACAT
TAATTTTTCATCTTTTTCAATACTGATTAAATTAATTGCTGGAATCCCTTTAGAGATTCT
AGAACCTAATGGAACTTGGTGAGCTCTTATTCGATAAACTTTACCATAGTTGGTAAAGAA
TAAAAGATCTGAGTGCGTTGAACACACGATTAATGATTCAACATCATCATCTTCGTATGT
GTTCATTCCTTTAACCCCAACACCACCACGGTGTTGAACTTTATAAGCACTAATCGGGAT
TCGTTTTAAATACCCTTTAGATGATCTAGTAATTACTACTGTCTCAACAGGTATTAACTG
TTCGTTATCGATCGTTGAATTAAGACCATAACAGATTTTGGTTCTTCTTTCATCACCAAA
CTTATGATCAATTTCATCAAGTTGATCAGAGATGATCTTAATTCTTCGTTCTTTGTTTTG
TAAGATCTCTTCTAGATCTTTAATTAGTTCTTTTAGTTTAGCTAATTCTTTTTGTAAGTT
TTCTCGTTCAAGTCCGCTTAAACTTCTTAAACGCATATCAAGAATCGCTTTTGCTTGCAG
ATCACTAAGTTCATACTTAGTCATTAAAGTGTTTTTAGCATCTTCATTATCCTTAGCATT
CTTAATGATCTCAATAACATCATCAATGTTATTAGTTGCGATTACCAAACCTTCAACGAT
ATGAGCTGAAGCTTTAGCTTTTTTCAGTTCGAAATTAGTTTTTCTTAATAAGATATCAAA
CTGATGATCGATATAGATTTGTAATGCTTCTTTAAGGTTAAGAACCTTAGGTTGGTTATT
TACCAAAGCAAGCATTGCTACTGAAAAATTAGTTTGTAACTGAGTTGATTTATATAGTTG
GTTTAATAAAACTTCAGGAATTACATCCCGCTTAGTTTCTATTACAATCCGAATCCCTTC
ACGGTTAGATTCATCACGTAGATCAGCAATCCCTTGAATCTGTTCTGTTTTTACTAGTTC
AGCAATCTTATTAATCAGATTGGCCTTGTTCACCATATAAGGGATCTGAGTAATAACAAT
ATTGCTTTTATTGTTAGCTAATTCTTCAATCTCAGATTTAGCCCGAACTGAAACAGAACC
TCGACCAGTATTGAAGTACTCATTAATTCCAGTTTCACCTAAGATCTCAGCTGCTGTGGG
AAAATCAGGACCCTTAATGAATTCTTTTAATTCCTCAATTGTCGCATTTTCATTCACTAA
TAAATGTTTAATCCCACCAATTAATTCGCTTAAATTATGTGGGGGAATATTAGTTGCCAT
CCCTACAGCAATACCACTTGAACCATTTGCTAATAAGTTAGGAAATAATGAAGGTAAAAC
AATCGGTTCTTGTTCTGAAGCATCATAGTTATCAACAAAATCAACAGTATCCTTATCGAT
ATTGCGCAACATCTCTGCCGAGATCTTAGATAATCTGGCTTCAGTATAACGCATCGCAGC
TGCGCTATCACCATCGATCGAACCAAAGTTACCATGCCCATCAATTAACATATATCTTAA
TGAAAATGGTTGTGCCATCCGCACCATGGTTTCATAAATCGCACTATCACCATGAGGGTG
GTATTTCCCCATAACGTGACCAACGATCTGAGCTGATTTACGATATGGTTTATCATGAGT
TAAACCTGAAGTGTAAGCACCATACAAAACCCGACGGTGCACTGGTTTTAAACCATCTCT
AGATTCAGGTAAAGCTCTTGAAACAATTACAGACATGGCATATTCCATGAATGATGTTTC
TAGCTCTTTAGTAATTGATGCTTCTTTAACTACTGTTTTATTTAACAGTTCTTTTATATT
GTTTTTATCGTTAGGATTCAT

>protein sequence
MNPNDKNNIKELLNKTVVKEASITKELETSFMEYAMSVIVSRALPESRDGLKPVHRRVLY
GAYTSGLTHDKPYRKSAQIVGHVMGKYHPHGDSAIYETMVRMAQPFSLRYMLIDGHGNFG
SIDGDSAAAMRYTEARLSKISAEMLRNIDKDTVDFVDNYDASEQEPIVLPSLFPNLLANG
SSGIAVGMATNIPPHNLSELIGGIKHLLVNENATIEELKEFIKGPDFPTAAEILGETGIN
EYFNTGRGSVSVRAKSEIEELANNKSNIVITQIPYMVNKANLINKIAELVKTEQIQGIAD
LRDESNREGIRIVIETKRDVIPEVLLNQLYKSTQLQTNFSVAMLALVNNQPKVLNLKEAL
QIYIDHQFDILLRKTNFELKKAKASAHIVEGLVIATNNIDDVIEIIKNAKDNEDAKNTLM
TKYELSDLQAKAILDMRLRSLSGLERENLQKELAKLKELIKDLEEILQNKERRIKIISDQ
LDEIDHKFGDERRTKICYGLNSTIDNEQLIPVETVVITRSSKGYLKRIPISAYKVQHRGG
VGVKGMNTYEDDDVESLIVCSTHSDLLFFTNYGKVYRIRAHQVPLGSRISKGIPAINLIS
IEKDEKLMSLLSINDYDSGYFFFSTRKGLVKRVKASEFSRIQNNGKIAIKLTENDSLFKV
IKTAGDEEIYIGVSSGLLVRFKEDAVRSMGRTAQGVIGVKFRNPNDEVIGLSSSHEGSLL
LAVCEKGVGKMTDREEYRMTNRGSKGVITIKVTPKTGNIINTQLVNGNEELLMISSTGKI
VRVPLAEVSEQGRNTSGVKLISLNEKETLQSVAIFDVGQDDSQQVGSDNPDTESSSDDSN
QDEDKE