GCW_90134

Uniprot: A0A0F6CLS8
Description: 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase
EC number: 4.6.1.12
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. {ECO:0000256|SAAS:SAAS00096238}.
Cofactor: COFACTOR: Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000256|SAAS:SAAS00611972};
Enzyme regulation:
Function [CC]: FUNCTION: Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). {ECO:0000256|SAAS:SAAS00349022}.
Pathway: PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6. {ECO:0000256|SAAS:SAAS00096241}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): ispF
Gene names (synonym):
Mass: 14,097
Subunit structure [CC]: SUBUNIT: Homotrimer. {ECO:0000256|SAAS:SAAS00248098}.
Gene ontology (GO): 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity [GO:0008685]; metal ion binding [GO:0046872]; isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway [GO:0019288]; terpenoid biosynthetic process [GO:0016114]
Gene ontology IDs: GO:0008685; GO:0016114; GO:0019288; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IspF family. {ECO:0000256|SAAS:SAAS00574101}.
Protein families: IspF family
Coiled coil:
Domain [FT]: DOMAIN 3 123 YgbB. {ECO:0000259|Pfam:PF02542}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHV85380
UniPathway: K01770
CDD:
Gene3D: cd00554
HAMAP: 3.30.1330.50
InterPro:
PANTHER: IPR003526;IPR020555
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01350
ProDom: PF02542
SMART:
SUPFAM:
TIGRFAMs: SSF69765
32862-33246(+)

>nucleotide sequence
GTGAAGACTGATCAACAATTGATTGCAAATTCAGATGGTGATGTAGTGCTTCATGCTTTA
AGCGATGCTGTGCTAGGGTGTGGTTCTTTTGGTGATATCGGTATGTATTTTGATGAAAAC
GATCTATCTAACAAAGGATTGGATTCAAAAACAATCTTAAATTACTGCTTAAAGTTGATT
AAAAAACTTAAGCTGGAATTTGTTAATATCGATCTAACAATCTTTGCCCAAGATATTAGA
ATCGATCCAATCAGATTTGAAATCAAAAGTTCTTTAATGAAATTAACAGGTTGTAACTCT
GTTAATGTCAAAGCTAAAAGTTACGAAGAACCTAAAAACGAAATTGCGTGTTCATGTGTA
GTTCTAATGAATACTAATAAGTAA

>protein sequence
VKTDQQLIANSDGDVVLHALSDAVLGCGSFGDIGMYFDENDLSNKGLDSKTILNYCLKLI
KKLKLEFVNIDLTIFAQDIRIDPIRFEIKSSLMKLTGCNSVNVKAKSYEEPKNEIACSCV
VLMNTNK