GCW_92037

Uniprot: A0A0F6CLY5
Description: Phenylalanyl-tRNA synthetase (PheRS) beta chain core domain
EC number: 6.1.1.20
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00022241}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00283}; ; Note=Binds 2 magnesium ions per tetramer. {ECO:0000256|HAMAP-Rule:MF_00283}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 464 464 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 470 470 Magnesium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 473 473 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 474 474 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.
Nucleotide binding:
Site:
Gene names (primary): pheT
Gene names (synonym):
Mass: 95,352
Subunit structure [CC]: SUBUNIT: Tetramer of two alpha and two beta subunits. {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00022357}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; phenylalanine-tRNA ligase activity [GO:0004826]; tRNA binding [GO:0000049]; phenylalanyl-tRNA aminoacylation [GO:0006432]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0000049; GO:0000287; GO:0004826; GO:0005524; GO:0005737; GO:0006432; GO:0008033
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00570605}.; SIMILARITY: Contains 1 B5 domain. {ECO:0000256|HAMAP-Rule:MF_00283}.; SIMILARITY: Contains 1 FDX-ACB domain. {ECO:0000256|HAMAP-Rule:MF_00283}.; SIMILARITY: Contains 1 tRNA-binding domain. {ECO:0000256|HAMAP-Rule:MF_00283}.
Protein families: Phenylalanyl-tRNA synthetase beta subunit family, Type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 40 161 TRNA-binding. {ECO:0000259|PROSITE:PS50886}.; DOMAIN 413 486 B5. {ECO:0000259|PROSITE:PS51483}.; DOMAIN 710 806 FDX-ACB. {ECO:0000259|PROSITE:PS51447}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHV85437
UniPathway: K01890
CDD:
Gene3D: cd02796
HAMAP: 2.40.50.140;3.30.56.20;3.30.70.380;3.50.40.10
InterPro: MF_00283
PANTHER: IPR005146;IPR009061;IPR012340;IPR004532;IPR020825;IPR005121;IPR002547;IPR033714;IPR005147
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51483;PS51447;PS50886
ProDom: PF03483;PF03484;PF03147;PF01588
SMART:
SUPFAM: SM00873;SM00874;SM00896
TIGRFAMs: SSF46955;SSF50249;SSF54991;SSF56037

In one protein complex with GCW_92036 481230-483705(+)

>nucleotide sequence
ATGTTGTTATCAAAAAAGGTAATTGCCAATTTCATTCCCACGATCACTAAGATCGAAGAT
AAAAAGATCGTTGAAGCACTCAACGCGATCGGTGCTGAGGTCGAATCAATTAAGACCTTT
AGTATGATCGATTACTTAATAATCGGAAAGATCTTTACGATTAAAAAACACCCCCATGCT
GAAAAACTAAATATCTGTTCGATCCAGATTAGTGATAATAAGTTTATTAACATCATCTCA
GATTCGCCCAACCTTTTTGACCAAACTAAGGTACTTAATAAGTATGTGATTGTGGCGATG
GAAGGTGCAGAACTACCCAACGGAATCACGGTAATTAACCGTGATATCCGGGGAATGAAT
AGTAGTGGGCTTTTATGTTCTTATGCTGATCTCAACCCACAATCAAGTGAGTATCTATCT
GAATATGATAGTAAGAATATCATTATCCTTGATAAGGCTAATTTAGGTGATACTGAAGTT
TATAAGTATCTAAATATCGATGATACGATCTTTGATATCTCGATCCCTTCTAGTCGTCCT
GATTGACATGGGATTAGATTTTTAGCTAAAGAATTAGCTGCTTATCTAAACTTAAAATAC
GTTGAACTAATTGGTAGAGCTAAACAAACTGATTTTCATCAGATTAATTTTAAGGTTTTA
GATGAAACAGATAATAAAGCCAAATACTTTGGTGGGATCCATTTAAGGAACTATCAATTA
CAACAATCAAGCTGAAACACCAAGGGGATCTTGATTAACAACCACATTAAACCTATTAAT
GATCTAGTTGATCTTTCTAATTTAATTCCGTTATTTGTGGCTAACCCAATCCACATTCAC
GACGCTGATAAGATCGTGGGTGATGTAAGATTAGTGCAAGCCACTAAAAAAGAGCAGTTT
TTAGCTTTAGATAATAAGTGATATACAGTTCAAGAGAATGATCTTTTAGTTGTTGATGAT
GAAAAGATCATTGCGCTTGCTGGGATCATTGGTTCAATGGCAACCGCTGTTGATGAGAAT
AGTATTAACTACTTTATTGAAGTAGGTAACTTTGATCGTCACCAGATCATTAAATCAGCA
GCATTTCATAAGATTAACACATACAGTGCTAATCTATTTAGTAAAGAAATCTCTTTATAC
CAAACCAAAAAGACCTTTGAATACTTATACCAATACTTATTGACAAAGGTTTATAATCAA
CAGTTATCTGAACTGTCAAAAACATTTAGTGTTGATGAGTATCATCAACAAGTAAAAGTT
AATTATGATAAGATTCGCAGCTTACTTGGAAGTGTGAACTACTTACCAGATGCTATGATC
CAAAAATCATTAACAGACCTAGGGTTTTTAGTTGAAGATGATCTTGTATACGTACCAAGT
TATCGAAACGATATTTATAACTGACAAGATCTAGTAGAAGAACTATTAAAGATCTTAAAT
ATTAATCTGTTCCAACCAATTCCGATTAAGGCTGATTATTTACTTGAAGTAAATAATGAA
ACAAACGAACTGTTAGATCGACTATCTAAAAAGTTAAGATCATTAAAGTTTAATCATATA
AGAACTTATAACTTAACTTCTAAAAAACGGGCTGAACTACTAAATCTGTTTAAGTATCAA
GATCCAGTTGTTGTGTCTAAACCAATCTCTGAAACTAGACAGTACTATCGTCAAAATATC
TTAACGAACTTATTAGAAGTTTATCAACTAAACCGATCATATAAGAATAAGTTATACCCA
ATCTTTGAGATCCAAAGTTTATTAACCAAAAATGGTGCTAACCACCATATTGGTTTAGTG
ATGGCTAATAACCTATTTAATCATAGTTATGATCCATCATCAGGGATCAAACTTGATCTA
ATCACGATCAAAGGGATTAGTGATATCATCGTGCAAAACTTTGGGTTTAATTGTAATTAC
CAAACGATTAATGATGATACTTATCTAGTTAAAAACGATTCATTAAAACTAGTGGTTTAT
GATGAAACGATCGGTTATATTGGTAAGATCAAAAAATCAGTTCTAAAAGAATTTGATTTA
GCTGATCAAGATATCTATTGTTTAGATATTAATCTGGAACGATTAATTACTTCAATTAAT
CGTTATGTCAGAACATATGAAGCTTATGATCACCACCAAGAAGTAACTAGAGACATTACT
TTCCAACTAAAAAATGAAGTTGATTTTAATAGCTTTATTAATGTCATTAATAGTTTTAAT
AAGTTATCAAAATGAGAGATTATCTCGATCTTTGATGCAACTAATCAGATTGATACGAAC
AAGACTTATCAACCAACCAAATATACGGTTAGATGTTATCTAAAACAAGGTGATAAGACT
TATACAACTAAAGAGATCAATCAAATCTTTGATGAATTGATTGATCTGATAGAAAACCAA
ACAGATCTTGATTTAGAATCGACGAATAATTTGATTAAATTTTATTTAAAAATAACGTTA
AATTTGCATAATTAA

>protein sequence
MLLSKKVIANFIPTITKIEDKKIVEALNAIGAEVESIKTFSMIDYLIIGKIFTIKKHPHA
EKLNICSIQISDNKFINIISDSPNLFDQTKVLNKYVIVAMEGAELPNGITVINRDIRGMN
SSGLLCSYADLNPQSSEYLSEYDSKNIIILDKANLGDTEVYKYLNIDDTIFDISIPSSRP
DWHGIRFLAKELAAYLNLKYVELIGRAKQTDFHQINFKVLDETDNKAKYFGGIHLRNYQL
QQSSWNTKGILINNHIKPINDLVDLSNLIPLFVANPIHIHDADKIVGDVRLVQATKKEQF
LALDNKWYTVQENDLLVVDDEKIIALAGIIGSMATAVDENSINYFIEVGNFDRHQIIKSA
AFHKINTYSANLFSKEISLYQTKKTFEYLYQYLLTKVYNQQLSELSKTFSVDEYHQQVKV
NYDKIRSLLGSVNYLPDAMIQKSLTDLGFLVEDDLVYVPSYRNDIYNWQDLVEELLKILN
INLFQPIPIKADYLLEVNNETNELLDRLSKKLRSLKFNHIRTYNLTSKKRAELLNLFKYQ
DPVVVSKPISETRQYYRQNILTNLLEVYQLNRSYKNKLYPIFEIQSLLTKNGANHHIGLV
MANNLFNHSYDPSSGIKLDLITIKGISDIIVQNFGFNCNYQTINDDTYLVKNDSLKLVVY
DETIGYIGKIKKSVLKEFDLADQDIYCLDINLERLITSINRYVRTYEAYDHHQEVTRDIT
FQLKNEVDFNSFINVINSFNKLSKWEIISIFDATNQIDTNKTYQPTKYTVRCYLKQGDKT
YTTKEINQIFDELIDLIENQTDLDLESTNNLIKFYLKITLNLHN