GCW_92579

Uniprot: A0A0F6CM08
Description: Ras-like GTPase superfamily protein ObgE, may regulate SpoT activity
EC number: 3.6.5.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01454, ECO:0000256|SAAS:SAAS00168993};
Enzyme regulation:
Function [CC]: FUNCTION: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. {ECO:0000256|HAMAP-Rule:MF_01454, ECO:0000256|SAAS:SAAS00319226}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 173 173 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01454}.; METAL 193 193 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01454}.
Nucleotide binding: NP_BIND 166 173 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.; NP_BIND 191 195 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.; NP_BIND 212 215 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.; NP_BIND 282 285 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.; NP_BIND 310 312 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.; NP_BIND 311 313 GTP. {ECO:0000256|HAMAP-Rule:MF_01454}.
Site:
Gene names (primary): obgE
Gene names (synonym): obg
Mass: 48,189
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01454, ECO:0000256|SAAS:SAAS00551999}.
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; magnesium ion binding [GO:0000287]; ribosome biogenesis [GO:0042254]
Gene ontology IDs: GO:0000287; GO:0003924; GO:0005525; GO:0005737; GO:0042254
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. {ECO:0000256|HAMAP-Rule:MF_01454, ECO:0000256|SAAS:SAAS00551984}.; SIMILARITY: Contains 1 G (guanine nucleotide-binding) domain. {ECO:0000256|HAMAP-Rule:MF_01454}.; SIMILARITY: Contains 1 OBG-type G (guanine nucleotide-binding) domain. {ECO:0000256|HAMAP-Rule:MF_01454}.
Protein families: TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family
Coiled coil:
Domain [FT]: DOMAIN 160 329 OBG-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51710}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHV85460
UniPathway: K03979
CDD:
Gene3D:
HAMAP: 2.70.210.12;3.40.50.300
InterPro: MF_01454
PANTHER: IPR031167;IPR014100;IPR015349;IPR006074;IPR006169;IPR006073;IPR027417;IPR005225
PIRSF: PTHR11702:SF3
PRINTS:
PROSITE: PR00326
Pfam: PS51710;PS00905
ProDom: PF09269;PF01018;PF01926
SMART:
SUPFAM:
TIGRFAMs: SSF102741;SSF52540;SSF82051
611433-612738(-)

>nucleotide sequence
CTATTCATCAATTAAATATTCATAATCACAGATAACGATTACATCATTAGGTTTGGCACC
CATCTTTTTTAGTTGGTCTTCAACACCTAATGATTTAATTTTCTGTCCTAATCTGGTGAT
ATTATCTAATGTAGTTTGAGGGATCTTCTTAAATCAATAAGTTAGTTTTTTTGATGAAAC
GATCCAACGGTTTTCCCCGTCTTTAATTACATCTAAAGGATCGTCATCAATCGTTTTTTC
ACCATCGTAGTAATAAACTTTTTCAGCAACCATCGGGATCGAAAACGGATTGGTTTCAGT
TGTTGTTGATAAGGTTTTTTGATAAAGATCAAAAACCCGATCAACTAAGTTATTGACATC
GCCATTGATCGCTGAGATGGAACTGATATCAATCCCTTTTTTAGCTAAATAACTTCTTAG
TTTATTGAAGTTTTCACTAGCACCTTCAACGTCCATCTTGTTAGCTACTACTAACATCTT
CTTATTAACTAGTAGTTGGGAATATTTTCTTAGTTCAGTCATGATCTTTTCATAAGCATC
GATCACATCATCATGGTCAAGTGGATTCATTGAGATTAAATGAATCAACACTTCACACCG
TTCAACGTGACGTAAGAAATCATGACCTAACCCATAACCTTCACTAGCATTTTCAATTAA
TCCTGGGATATCAGCAAACACTAAGCGTTTGTCATTATTTTCAACGATCCCCAAGATTGG
TACTAACGTTGTAAACTGGTAGTTAGCAATCTTTGGTTTGGCATTCGATAATTTGGAAAT
CAACGTTGATTTTCCCGCATTGGGATAACCAACAATCCCCACGTTGGCTAAATATTTTAG
TTCTAACCTAACAGTTTTTTCTTCACCTTCATCACCTAACTCATATAAGTTAGGTGCGCG
TAAGATTGATGATTTAAAAGCCGCATTACCTCGACCACCCTTACCACCGTGACAGATAAT
ATATTCTTGTTTATCTCTGATCAGATCTACGATTACTTCATTAGTTTGTTCATCATAAAC
GGTGGTACCAATCGGAACTTTAACATACTTATCCATCCCGTTTTGACCTGATGATAGATC
TGGTTTACCATTATCACCATTACTTGCACGGATGATCTTTGAATATTTCAAACTTAATAA
GGTTGATTGGTTGTGATCAGCTACCAGAATGATATTACCACCCTTACCACCACTTCCACC
AGCAGGTCCACCCTTATCATAGTGTGCTTCTCTTCTTCAGGCAATGATCCCATCACCACC
ATTACCAGCAATTAGTTTTATTTGACATCGATCGATAAATTGCAT

>protein sequence
MQFIDRCQIKLIAGNGGDGIIAWRREAHYDKGGPAGGSGGKGGNIILVADHNQSTLLSLK
YSKIIRASNGDNGKPDLSSGQNGMDKYVKVPIGTTVYDEQTNEVIVDLIRDKQEYIICHG
GKGGRGNAAFKSSILRAPNLYELGDEGEEKTVRLELKYLANVGIVGYPNAGKSTLISKLS
NAKPKIANYQFTTLVPILGIVENNDKRLVFADIPGLIENASEGYGLGHDFLRHVERCEVL
IHLISMNPLDHDDVIDAYEKIMTELRKYSQLLVNKKMLVVANKMDVEGASENFNKLRSYL
AKKGIDISSISAINGDVNNLVDRVFDLYQKTLSTTTETNPFSIPMVAEKVYYYDGEKTID
DDPLDVIKDGENRWIVSSKKLTYWFKKIPQTTLDNITRLGQKIKSLGVEDQLKKMGAKPN
DVIVICDYEYLIDE