GCW_92786

Uniprot: A0A0F6CM11
Description: Ribonuclease III
EC number: 3.1.26.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231310}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231322};
Enzyme regulation:
Function [CC]: FUNCTION: Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. {ECO:0000256|HAMAP-Rule:MF_00104}.
Pathway:
Active site: ACT_SITE 35 35 {ECO:0000256|HAMAP-Rule:MF_00104}.; ACT_SITE 104 104 {ECO:0000256|HAMAP-Rule:MF_00104}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 31 31 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 101 101 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 104 104 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.
Nucleotide binding:
Site:
Gene names (primary): rnc
Gene names (synonym):
Mass: 24,517
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231419}.
Gene ontology (GO): cytoplasm [GO:0005737]; metal ion binding [GO:0046872]; ribonuclease III activity [GO:0004525]; rRNA binding [GO:0019843]; mRNA processing [GO:0006397]; rRNA catabolic process [GO:0016075]; rRNA processing [GO:0006364]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0004525; GO:0005737; GO:0006364; GO:0006397; GO:0008033; GO:0016075; GO:0019843; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain. {ECO:0000256|HAMAP-Rule:MF_00104}.; SIMILARITY: Contains 1 RNase III domain. {ECO:0000256|HAMAP-Rule:MF_00104}.
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 1 115 RNase III. {ECO:0000259|PROSITE:PS50142}.
Motif:
Region:
EMBL: CP006916
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: AHV85463
UniPathway: K03685
CDD:
Gene3D: cd00593
HAMAP: 1.10.1520.10;3.30.160.20
InterPro: MF_00104
PANTHER: IPR014720;IPR011907;IPR000999
PIRSF:
PRINTS:
PROSITE:
Pfam: PS00517;PS50142
ProDom: PF00035;PF14622
SMART:
SUPFAM: SM00535
TIGRFAMs: SSF69065
662259-662904(-)

>nucleotide sequence
TTATTTTTCCAGCTTTAGTAGGCCACGAAATTTGCTAATCGCGTCTTTTGCTGCTTTTAT
TTCTGCTTTGTGGGTGCTTGAATCCTTACCTTCACCATACATGATCTCACCTGCATAAAG
ACTTACTGTATGAAGGTTCTTTTGTCGATCATAGGTTCTTTCGTAATGGATTTTCATGTT
CAATCCTGTTGAGTGAATAATTTCTTGGAAAATTGACTTATAATCAGTGTTTTCAGTTAA
TTGACCTTTTTGGTGATAACCTATGATGGTATCATTTAAGATCTTTCTTACTGCATACTC
ACCTTGATCTAAATACATAGCTCCAATAAATGCTTCAAAGATATCTTCAAGGATCTTTGG
TGATAAAGGAGCAATTTTTAATCCTTCACCAACCCTAATATAATTAATTAGTTCTAATTG
GGTAGCTGCTTTAATCAGTGTATTAGATTGAATGATCTTTATACGATCTTTAGTCATTTC
ACCTTCAGATGAATCTGAATGATTAAATAAATACTCTGCAACTATCTTATTAATGATTGC
ATCACCTAAGAACTCTAATCTTTGATAATTGTATTTTAAATGCATCTCATTAGCGTATGA
GTTGTGTGTTAGCGCTTCTTCATAAAGTCCAATGTTGTTTGTCAC

>protein sequence
VTNNIGLYEEALTHNSYANEMHLKYNYQRLEFLGDAIINKIVAEYLFNHSDSSEGEMTKD
RIKIIQSNTLIKAATQLELINYIRVGEGLKIAPLSPKILEDIFEAFIGAMYLDQGEYAVR
KILNDTIIGYHQKGQLTENTDYKSIFQEIIHSTGLNMKIHYERTYDRQKNLHTVSLYAGE
IMYGEGKDSSTHKAEIKAAKDAISKFRGLLKLEK