SPM_000060

Uniprot: A0A037UKQ8
Description: threonyl-tRNA synthase
EC number: 6.1.1.3
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). {ECO:0000256|HAMAP-Rule:MF_00184, ECO:0000256|SAAS:SAAS00230769}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00184}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00184}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 330 330 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00184}.; METAL 381 381 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00184}.; METAL 511 511 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00184}.
Nucleotide binding:
Site:
Gene names (primary): thrS
Gene names (synonym):
Mass: 73,442
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00184}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; metal ion binding [GO:0046872]; threonine-tRNA ligase activity [GO:0004829]; threonyl-tRNA aminoacylation [GO:0006435]
Gene ontology IDs: GO:0004829; GO:0005524; GO:0005737; GO:0006435; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00184, ECO:0000256|SAAS:SAAS00557708}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 268 534 AA_TRNA_LIGASE_II. {ECO:0000259|PROSITE:PS50862}.
Motif:
Region: REGION 237 534 Catalytic. {ECO:0000256|HAMAP-Rule:MF_00184}.
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92514
UniPathway:
CDD:
Gene3D: cd00771
HAMAP: 3.10.20.30;3.40.50.800
InterPro: MF_00184
PANTHER: IPR002314;IPR006195;IPR004154;IPR012675;IPR004095;IPR012676;IPR002320;IPR018163;IPR033728
PIRSF:
PRINTS:
PROSITE: PR01047
Pfam: PS50862
ProDom: PF03129;PF02824;PF00587
SMART:
SUPFAM:
TIGRFAMs: SSF52954;SSF55186;SSF81271

8316-10230(-)

>nucleotide sequence
TTAAATTTTATTAATAATTTGCTCATTAATTAAAATAATAAATTTATCAAAAGTAGTAGT
AATTTGATCTTCTTGCCCGTAAATTCGATAAGTAATCATTTTTTTATCCCGTTCTTGATC
ACCAATAACGATTTGATATGGAATTTTAGCAACTTGTGCTTCTCGGATTTTATAACTTAA
ACGTTCATCACGATCATCAATTGTTGTACGTAATTTTAACTTTTTAAATTGCCGGTGTAA
TTCATTAACATATTCCAAATGATGCTCATTATTAACTGGAATTATGACAATTTGTTTTGG
AGCTAATCAAAATGGTAAAACTCCTTTTGTTTGTTCTAATAAAATTGCTATAAAACGCTC
ATAAGTCCCAATTAAACCACGATGTAACATTACTGGCATTGCTTTTGCCCCTGTTGCATC
AATATAACTTAAATTAAATTTTTTTGGTAATAAAAAATCAAATTGTAAGGTTGAAACAGT
AATTTCATGGTTTAATGCTGTCTTAATTTGAATATCCAACTTTGGTCCATAAAATGCTGC
TTCTCCAATCATTGGCACATATGGAATTTTTAAATCATCTAATGCTTCTTGCAACATTTG
TTCAGCATGATTTCACATTTGATTATCATCATAATATTTTTCCTTATTTGCTGGATCACG
TAATGACAATGAGTAATAATCAACAGTAATTTTTAAATCTGTTAAAACTTCATTAATTAA
TTTAAAACAACGTTTAAACTCTTCTTTCAACTGGTCATGACGTAAAAAGATATGCGAATC
AGTTAATTGCATCATTCGTACTCGTTCTAGACCTGTTAAACTTCCTGATGTCTCATATCG
ATGTAAAATAGCATGCTCAGCAATTCGTAATGGTAACTCACGATAACTACGTTGTTTATA
ATTATAAACAGCAATATGATGAGGGCAAGCCATTGGGCGTAAAACACTAATTTCATTATC
TTGTGCCATCGGGGCAAACATATTTTCTTGGTAATGATCCCAGTGACCAGAAATTTTATA
CATTTCACTAGTTCCAATAACTGGTGTATCAACCTCAATAAAATCATATTCTCATTCTTT
TTCTCGGATATATTCTTGGAGCACTTTTTTTAATGCCATCCCATTTGGTAACCAAATCGG
TAAACCGGGTCCAACTAATTTATCAAAAGTAAAAATTTCTAAATCCTTCCCAATTTTACG
ATGATCACGCTCTCTTCGTTCTTCATATGCGAATAATACTTGTTCAAATAATTCACGTGA
AAAATGTGAACTTCCAGTTAATCGTTGCAACATTTTATTTTTAGCATCATCTTGTCAATA
TGCTCCTGCTAAGCTTCAAAGTTTCAGGACTTTAATATGTTTCAAACTTGTAACTGGATA
AGTATTTGGCAAATACAATTCATTCCCAAGTAAATATCCACCTTGTTCTGCCCCTGTTTC
TGCACAAAAAGTTAATAAATAGGGATTGTTATTATATCATTTTTTTGCATCGGCTAGTGC
CCCTAAAACAGGCTTAATAGCAAGATTATTATTAATTAACTTTGTTACTTCCTGTTCTAA
AGCACTAAGATCAGTTTCTTTTAAATGTGGTTCCACATCAAAGTCAACTAAAAAGGTATC
ATCTTGTACGGCAATTTTAGCAATTTTAGCGGTTGGATGTAAATTAGTAATTGCCTTTGC
TGTTATTAATGCTGTTGTATAATGCAAAATTGCTAAAGCTAGCGGTGATTTATCAGTAAT
AATTTCTAATGTTCCATCAGTTGTTACTAAATAGTCTAAACCACGAACTTGACCAGCAAA
AACTCCACCAAAGGCAGCTTTTCCTAAACTTGATGCAATACTACTTGCTATAGCATGAAT
TGTTTGTGGTGTCTTAAATTCACGAACTTGACCATCCGGTAATGTAATTTTAAT

>protein sequence
IKITLPDGQVREFKTPQTIHAIASSIASSLGKAAFGGVFAGQVRGLDYLVTTDGTLEIIT
DKSPLALAILHYTTALITAKAITNLHPTAKIAKIAVQDDTFLVDFDVEPHLKETDLSALE
QEVTKLINNNLAIKPVLGALADAKKWYNNNPYLLTFCAETGAEQGGYLLGNELYLPNTYP
VTSLKHIKVLKLWSLAGAYWQDDAKNKMLQRLTGSSHFSRELFEQVLFAYEERRERDHRK
IGKDLEIFTFDKLVGPGLPIWLPNGMALKKVLQEYIREKEWEYDFIEVDTPVIGTSEMYK
ISGHWDHYQENMFAPMAQDNEISVLRPMACPHHIAVYNYKQRSYRELPLRIAEHAILHRY
ETSGSLTGLERVRMMQLTDSHIFLRHDQLKEEFKRCFKLINEVLTDLKITVDYYSLSLRD
PANKEKYYDDNQMWNHAEQMLQEALDDLKIPYVPMIGEAAFYGPKLDIQIKTALNHEITV
STLQFDFLLPKKFNLSYIDATGAKAMPVMLHRGLIGTYERFIAILLEQTKGVLPFWLAPK
QIVIIPVNNEHHLEYVNELHRQFKKLKLRTTIDDRDERLSYKIREAQVAKIPYQIVIGDQ
ERDKKMITYRIYGQEDQITTTFDKFIILINEQIINKI