SPM_000110

Uniprot: A0A037UPI1
Description: ribonuclease HIII
EC number: 3.1.26.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester. {ECO:0000256|RuleBase:RU003515, ECO:0000256|SAAS:SAAS00088232}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|SAAS:SAAS00611738}; ; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|SAAS:SAAS00611737};
Enzyme regulation:
Function [CC]: FUNCTION: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. {ECO:0000256|RuleBase:RU003515, ECO:0000256|SAAS:SAAS00088233}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary):
Gene names (synonym):
Mass: 33,966
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; metal ion binding [GO:0046872]; RNA binding [GO:0003723]; RNA-DNA hybrid ribonuclease activity [GO:0004523]
Gene ontology IDs: GO:0003723; GO:0004523; GO:0005737; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNase HII family. {ECO:0000256|RuleBase:RU003515}.
Protein families: RNase HII family
Coiled coil:
Domain [FT]: DOMAIN 8 75 DUF3378. {ECO:0000259|Pfam:PF11858}.; DOMAIN 91 295 RNase_HII. {ECO:0000259|Pfam:PF01351}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92523
UniPathway:
CDD:
Gene3D:
HAMAP: 3.30.420.10
InterPro:
PANTHER: IPR001352;IPR024567;IPR004641;IPR024568;IPR012337
PIRSF: PTHR10954
PRINTS: PIRSF037748
PROSITE:
Pfam:
ProDom: PF11858;PF01351
SMART:
SUPFAM:
TIGRFAMs: SSF53098

18607-19507(+)

>nucleotide sequence
ATGAATAATATCAGTTTTAAAAAAGTTGATTCTACAATTATTAAAGCGATTAGTACTGCT
TATCAACCTTATGCAATTAATAATACAGATCCCAATAAAGTTAGTGTTTTTAATAAAAAT
GGAATTATTATTACAATTTATAAAACAAAAAGTGTTTTGTTCCAAGGCTATCAAGCTGAA
AAAGAAGCACAGCGTTTTTTTCCAGCCTTAACCAGTTCTTTACCAAATAAAAATGTCTCT
GCTCCAGTTACATATTTTCAAAAAGATGTTATTGGTAATGACGAAGTTGGTGTTGGCGAT
ATTTTTGGTCCGCTAGTTGTTACCGCTTCTTATCTTCCTATTGCAACTTTTAATGAATTA
GCAAAGTTACCAATCAAAGATAGTAAAAAGATGACAAAACAACAAATTTTAAGTTTAGCC
CCAAAAATTAAAAAAATGGTCAAAAGTATCACTATTATTATTAATAATGAACTTTATAAT
AAATGATATACTAAATACCAAAACGCCCATATTATTAAAACCTTAGCCCATAATCAAGCT
TTAGTCCAATTATTAGCAAAACATCAGTTAACTAATAAAACAATTTTTATTGACCAATTT
GTTAATGAAACAAAATATTTTGAATATTTACAACAAACTAAAATGAGTTCAATTATAAAA
GATAACCTTGTTTTTATCACTAAAGGAGAAGAAAAATCATTAGCCATTGCTTGTAGCGCT
ATTTTAAGTCGTGCAGTCTTTCTAATTAAAATTAATGAACTTGAAACAAAATATCATTTA
TCATTCCCCTTAGGAGCCAGTGAAGATGTTAAAGCACTAGCCCGTAAATATAAAGAAATA
ATGCCAACAACTACTTATACTCAATTTTTAAAAGAGCATTTTAATTTAACAAAAAAATAA

>protein sequence
MNNISFKKVDSTIIKAISTAYQPYAINNTDPNKVSVFNKNGIIITIYKTKSVLFQGYQAE
KEAQRFFPALTSSLPNKNVSAPVTYFQKDVIGNDEVGVGDIFGPLVVTASYLPIATFNEL
AKLPIKDSKKMTKQQILSLAPKIKKMVKSITIIINNELYNKWYTKYQNAHIIKTLAHNQA
LVQLLAKHQLTNKTIFIDQFVNETKYFEYLQQTKMSSIIKDNLVFITKGEEKSLAIACSA
ILSRAVFLIKINELETKYHLSFPLGASEDVKALARKYKEIMPTTTYTQFLKEHFNLTKK