SPM_000205

Uniprot: A0A037UPM2
Description: phenylalanine--tRNA ligase
EC number: 6.1.1.20
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). {ECO:0000256|HAMAP-Rule:MF_00281, ECO:0000256|SAAS:SAAS00089465}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00281}; ; Note=Binds 2 magnesium ions per tetramer. {ECO:0000256|HAMAP-Rule:MF_00281}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 257 257 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00281}.
Nucleotide binding:
Site:
Gene names (primary): pheS
Gene names (synonym):
Mass: 39,400
Subunit structure [CC]: SUBUNIT: Tetramer of two alpha and two beta subunits. {ECO:0000256|HAMAP-Rule:MF_00281, ECO:0000256|SAAS:SAAS00089470}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; phenylalanine-tRNA ligase activity [GO:0004826]; tRNA binding [GO:0000049]; phenylalanyl-tRNA aminoacylation [GO:0006432]
Gene ontology IDs: GO:0000049; GO:0000287; GO:0004826; GO:0005524; GO:0005737; GO:0006432
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_00281, ECO:0000256|SAAS:SAAS00541523}.
Protein families: Class-II aminoacyl-tRNA synthetase family, Phe-tRNA synthetase alpha subunit type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 21 84 Phe_tRNA-synt_N. {ECO:0000259|Pfam:PF02912}.; DOMAIN 92 341 tRNA-synt_2d. {ECO:0000259|Pfam:PF01409}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92541
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00281
PANTHER: IPR004529;IPR004188;IPR022911;IPR002319;IPR010978
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF02912;PF01409
SMART:
SUPFAM:
TIGRFAMs: SSF46589

37256-38294(-)

>nucleotide sequence
CTAATCAAATTTCTTAAATTGTTCTAAAAAGCGGATGTCATTCGTATAAAAACGACGAAT
ATCATCAATTCCATATTTTAACATCGCAATTCGTTCAATTCCAATCCCAAAAGCAAAGCC
AGTTAATGTTGGATCTTGACCACATGCTTCATAAACAAGCGGATTAATCATCCCAGACCC
CATAATCTCAATTCATCCAGTTTGCTTGCAAATTGAACAACCTTTACCTTGACAATTAAC
ACAAGTAACATCAACTTCAACTGACGGTTCTGTAAATGGAAAAAAACTTGGCCGTAAACG
GATTTGCGTTGCTTCACCAAACATTCTTTGACAAAAGTATTGAATTGTTCATTTTAAATT
AGCAAAAGTAATATTTGGAGCAACTAAAAATCCATCAATTTGCATAAATTGGTGTGAATG
CGTAGCATCATCATCATCCCGACGATAAGTATTTCCGGCTGAAATCATCGCAATAATTTC
GTTTTTACTTTTCATCTGCGAAATTGCACGGGCTGTCATGTTGGTACAATGTGTTCGTAA
TAATGTATTTTTAGACAAATAAAATGTATCTTGCATATCACGAGCGGGATGCCCTAATGG
TAAATTTAAGCGTTGAAAATTATATTCATCATCATCAACTTCAGTTCCAAACACAATATC
ATATCCTAATTCTTGGAATAATTGAGAAATTTCATCAATAACTTGGTTTAAAGGATGCTT
TGTTGCTAACACTAAATTATATCCTGGTAACGACAAATCAATTTTTTCTTGTTCAATTGT
TGCTAATAATAAAGCATTTTCTAATTCTGTCTTTTTAGTTTGACATAATACGGTTAATTC
CCCTTTAATTTGATTTGCTTTTTGTCCAATTGTTAAACGTTCTTGATGCGTTAAATCTTT
CATTTTTTTCAATAAATCATTCAACATTGCTTTTTTTCCCAAATATTTTAATTGAAGCGT
ATTAACTTCCGCTACTGTTTTTGCTGCTTTAATTTTTTTTGTTGCTTGATTAAATAATAA
TTCTAATTCTTTTTCCAT

>protein sequence
MEKELELLFNQATKKIKAAKTVAEVNTLQLKYLGKKAMLNDLLKKMKDLTHQERLTIGQK
ANQIKGELTVLCQTKKTELENALLLATIEQEKIDLSLPGYNLVLATKHPLNQVIDEISQL
FQELGYDIVFGTEVDDDEYNFQRLNLPLGHPARDMQDTFYLSKNTLLRTHCTNMTARAIS
QMKSKNEIIAMISAGNTYRRDDDDATHSHQFMQIDGFLVAPNITFANLKWTIQYFCQRMF
GEATQIRLRPSFFPFTEPSVEVDVTCVNCQGKGCSICKQTGWIEIMGSGMINPLVYEACG
QDPTLTGFAFGIGIERIAMLKYGIDDIRRFYTNDIRFLEQFKKFD