SPM_000245

Uniprot: A0A037UPM7
Description: GTP-binding protein LepA
EC number: 3.6.5.n1
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: GTP + H(2)O = GDP + phosphate. {ECO:0000256|HAMAP-Rule:MF_00071}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner. {ECO:0000256|HAMAP-Rule:MF_00071}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 16 21 GTP. {ECO:0000256|HAMAP-Rule:MF_00071}.; NP_BIND 133 136 GTP. {ECO:0000256|HAMAP-Rule:MF_00071}.
Site:
Gene names (primary): lepA
Gene names (synonym):
Mass: 67,612
Subunit structure [CC]:
Gene ontology (GO): plasma membrane [GO:0005886]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; ribosome binding [GO:0043022]; translation elongation factor activity [GO:0003746]; positive regulation of translation [GO:0045727]
Gene ontology IDs: GO:0003746; GO:0003924; GO:0005525; GO:0005886; GO:0043022; GO:0045727
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily. {ECO:0000256|HAMAP-Rule:MF_00071}.
Protein families: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily
Coiled coil:
Domain [FT]: DOMAIN 4 186 Tr-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51722}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92546
UniPathway:
CDD:
Gene3D:
HAMAP: 3.30.70.240;3.40.50.300
InterPro: MF_00071
PANTHER: IPR006297;IPR009022;IPR000640;IPR031157;IPR013842;IPR027417;IPR005225;IPR000795;IPR009000
PIRSF:
PRINTS:
PROSITE: PR00315
Pfam: PS00301;PS51722
ProDom: PF00679;PF06421
SMART:
SUPFAM: SM00838
TIGRFAMs: SSF50447;SSF52540;SSF54980

48297-50100(-)

>nucleotide sequence
TTAATCATCTAACTTTAAAACAGCCATAAAAGCTTCTTGAGGTACTTCAACAGAACCAAT
TGCTTTCATTCGTTTTTTTCCTTCTTTTTGTTTTTCTAACAATTTCTTTTTCCGGGTAAT
ATCGCCACCATAACATTTTGCTAATACATTTTTCCGCATAGCTTTAATATCTTCTCGCGC
AATAACTTTATGATTAATGGCTGCTTGAATTGGAACTTCAAAATTTTGACGGGGAATAAT
TTCTTTTAACTTTGCACAGAGAGCTTTACCACGGCCATAAGCAAAATCACGGTGAACAAT
AATTGATAATGCATCAATAATTTCATTGTTTAGTAAAATATCCATTTTAACTAATTTATT
AGGACGATAACCAATAAACTCATAATCTAACGAAGCATAACCTTTACTAATTGATTTTAA
CCGATCAAAAAAATCAAAAACAATTTCATTTAATGGCATCTCATAAGTCAAAATTCGTCG
CGTATCATCAATATACTCTAAATTAACATATGTCCCACGTTTATTTTGACATAATTCCAT
TAATGAACCAATATATTGTTCGGGAGTCATAATTGTTGCTTTGACAAATGGTTCTTCGAT
GCGATCAATTTTTTGCACAGCGGGTAATTCACTTGGATTATCAATGCTAATCATTTCCTT
ATTTGTTAAATAAACATGATAAATTACCGATGGTGCAGTTGCAATTAACTCTAAATTATA
TTCTCGTTCTAATCGTTCTTGGATTACATCCATATGTAATAATCCTAAAAAGCCACAACG
AAAACCAAAACCTAATGCTTGCGATGTCTCTGGCTCATAAACAAGGGAAGCATCCGATAA
CTGAATTTTTTCCAAGGCTTCTTTTAAATCTTTATATTTAGCAGAATCAATTGGGTATAG
GCCACAAAACACCATTGGGTTCATTTTTTTATATCCTGGCAATGGCTGTTGCGCTGGATT
AGCAACAGTTGTAATCGTATCACCAACCCGAACATCACGCACCATTTTAATACTTGCTGC
TAATCACCCTACTTCACCGGCTCCTAATTGGTTTTTTTTAACTTCTTTTGGGGTTTTAAC
TCCTAATTCGGTAACTTCATAAACAGCACCGGTATTCATTAATTTAATTTTTTCACCAAC
GCGAACAATTCCTTGCTTAACTCGAATTGAGACCATTACGCCTCGGTATTTATCATAATA
TGAATCAAAAATTAATGCTTGCAACGGATTATTATCATCGCCGTCTAATGGGGCAGGAAT
ATCCTTGACAATTGCTTCTAATACCTGTTCAATATTTAATCCTGTTTTAGCACTAATTAA
TGGTGCATTTGTTGTTGGAATTCCAATTAAATTTTCAATTTCTTCTTTAACGCGGTCTGG
ATCGGCTGATGGTAAATCAATTTTATTAATAACGGGAATAATTGCTAAATTATTATCAAT
TGCTAAATAAACATTAGCTAATGTTTGAGCTTCAATTCCTTGTGCTGCATCAACAACTAA
AATTGCTCCTTCACAAGCTGCTAAACTACGTGATACTTCATAAGTAAAATCAACATGTCC
TGGAGTATCAATTAAATGAAAAATATACTCTTGTTGATCTTGAGCCCGATACTTTAACTG
AACAGAATTTAATTTAATTGTTATTCCTCGTTCACGTTCTAAATCCATTGAATCCAATAA
TTGTTCTTGCATTTCTCGTTTTTCAACTGTTCCTGTTAACTCTAAAATCCGGTCTGCTAA
GGTTGACTTTCCATGGTCAATATGAGCAATAATTGAAAAATTACGAATTAATTTTTTATC
CAT

>protein sequence
MDKKLIRNFSIIAHIDHGKSTLADRILELTGTVEKREMQEQLLDSMDLERERGITIKLNS
VQLKYRAQDQQEYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYL
AIDNNLAIIPVINKIDLPSADPDRVKEEIENLIGIPTTNAPLISAKTGLNIEQVLEAIVK
DIPAPLDGDDNNPLQALIFDSYYDKYRGVMVSIRVKQGIVRVGEKIKLMNTGAVYEVTEL
GVKTPKEVKKNQLGAGEVGWLAASIKMVRDVRVGDTITTVANPAQQPLPGYKKMNPMVFC
GLYPIDSAKYKDLKEALEKIQLSDASLVYEPETSQALGFGFRCGFLGLLHMDVIQERLER
EYNLELIATAPSVIYHVYLTNKEMISIDNPSELPAVQKIDRIEEPFVKATIMTPEQYIGS
LMELCQNKRGTYVNLEYIDDTRRILTYEMPLNEIVFDFFDRLKSISKGYASLDYEFIGYR
PNKLVKMDILLNNEIIDALSIIVHRDFAYGRGKALCAKLKEIIPRQNFEVPIQAAINHKV
IAREDIKAMRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKAIGSVEVPQEAFMAVLKLDD