SPM_000390

Uniprot: A0A037UPR1
Description: lactate dehydrogenase
EC number: 1.1.1.27
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH. {ECO:0000256|HAMAP-Rule:MF_00488}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. {ECO:0000256|HAMAP-Rule:MF_00488}.
Active site: ACT_SITE 179 179 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00488, ECO:0000256|PIRSR:PIRSR000102-1}.
Binding site: BINDING 92 92 Substrate. {ECO:0000256|HAMAP-Rule:MF_00488}.; BINDING 124 124 NAD or substrate. {ECO:0000256|HAMAP-Rule:MF_00488}.; BINDING 155 155 Substrate. {ECO:0000256|HAMAP-Rule:MF_00488}.; BINDING 232 232 Substrate. {ECO:0000256|HAMAP-Rule:MF_00488}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 14 42 NAD. {ECO:0000256|HAMAP-Rule:MF_00488}.
Site:
Gene names (primary): ldh
Gene names (synonym):
Mass: 34,337
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00488}.
Gene ontology (GO): cytoplasm [GO:0005737]; L-lactate dehydrogenase activity [GO:0004459]; glycolytic process [GO:0006096]
Gene ontology IDs: GO:0004459; GO:0005737; GO:0006096
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family. {ECO:0000256|HAMAP-Rule:MF_00488}.
Protein families: LDH/MDH superfamily, LDH family
Coiled coil:
Domain [FT]: DOMAIN 7 145 Ldh_1_N. {ECO:0000259|Pfam:PF00056}.; DOMAIN 149 315 Ldh_1_C. {ECO:0000259|Pfam:PF02866}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal: A0A037UPR1
MEROPS:
EnsemblBacteria KO: KAI92571
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.720;3.90.110.10
InterPro: MF_00488
PANTHER: IPR001557;IPR011304;IPR018177;IPR022383;IPR001236;IPR015955;IPR016040
PIRSF: PTHR11540
PRINTS: PIRSF000102
PROSITE: PR00086
Pfam: PS00064
ProDom: PF02866;PF00056
SMART:
SUPFAM:
TIGRFAMs: SSF51735;SSF56327

76982-77939(-)

>nucleotide sequence
TTATTTTAGCGCTGCAAGAGCAACATTTAAAGCTTCATGTAATTGTGTCGCTGATTTTTC
AAATTGAACTTGCTCTTCTTTTGCTAATTTTCATTCAATAATTTCTTCAATTCCATTCGC
CCCAATAACGGCTGGGACACCAATGTATGTTCCCTTAACATTATATTCGCCTTCACATTT
TGCACCAACTAAGAACGTTGATCTTTCATCATTTAAAATTGCTTTTGCAATTGATGATAA
ACATACCCCAATTCCATAAAAAGTTGCTTTCTTTAAATCAATGATTGTATAAGCCATTTT
CATCATTTGTTGATGCATATCTTCTAAATCTTTTTCTGTTAATTTTCCTTCTGCAACAAA
ATCAGCAATTGATTTTTGCATAATTGAAGCTTTTGATCAAGCAGAAACTGATGAATCACC
ATGTTCACCCAAAACATAGGCATTAACACTAGCTGGAGCAATATTTAATTTATTACCAAC
TAAGCGTCTTAAACGTGCTGAATCTAAAGTTGTTCCTGAAGAAATAACTTTATTTTTTGC
ATAACCAGTCACTTTTTGATAAACTGACGCCAAAACATCGACCGGATTTGAAGCAATTAG
AGTAACCCCTTTAAAACCTGACGCTTTAATTTTTAAAGCAATATCTTGCATAATTCTTGC
ATTATCAGCAACCATATCTAAACGTGTTTCACCATCTCGTTGTGGTCTGCCAGCTGTAAC
AACAATTAAATCAGCATCTTTACATTCACTATAATCACCAGCTTTAATTGTACTAAAACG
ATTTTCTAAAACAGCAACAGCATCTGATAAATCAATAGCGTTCCCTTCTGCTGCTTGTCG
AAAGGCATCAATTAACACATAATGTTGTGCAATCCCTCTATTCATCGCTGAATATAAAAA
GCTGTTTCCAACCATTCCACATCCAACTAAAACTACTTTTCTCGTTGAATTTTCCAT

>protein sequence
MENSTRKVVLVGCGMVGNSFLYSAMNRGIAQHYVLIDAFRQAAEGNAIDLSDAVAVLENR
FSTIKAGDYSECKDADLIVVTAGRPQRDGETRLDMVADNARIMQDIALKIKASGFKGVTL
IASNPVDVLASVYQKVTGYAKNKVISSGTTLDSARLRRLVGNKLNIAPASVNAYVLGEHG
DSSVSAWSKASIMQKSIADFVAEGKLTEKDLEDMHQQMMKMAYTIIDLKKATFYGIGVCL
SSIAKAILNDERSTFLVGAKCEGEYNVKGTYIGVPAVIGANGIEEIIEWKLAKEEQVQFE
KSATQLHEALNVALAALK