SPM_000495

Uniprot: A0A037UKZ1
Description: N-acetylmuramic acid-6-phosphate etherase
EC number: 4.2.1.126
Annotation score: 3 out of 5
Miscellaneous [CC]: MISCELLANEOUS: A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product. {ECO:0000256|HAMAP-Rule:MF_00068}.
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510857}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00570226}.
Pathway: PATHWAY: Amino-sugar metabolism; N-acetylmuramate degradation. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510845}.
Active site: ACT_SITE 86 86 Proton donor. {ECO:0000256|HAMAP-Rule:MF_00068}.; ACT_SITE 117 117 {ECO:0000256|HAMAP-Rule:MF_00068}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): murQ
Gene names (synonym):
Mass: 32,182
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510849}.
Gene ontology (GO): carbohydrate binding [GO:0030246]; carbon-oxygen lyase activity [GO:0016835]; carbohydrate metabolic process [GO:0005975]; N-acetylmuramic acid catabolic process [GO:0097173]
Gene ontology IDs: GO:0005975; GO:0016835; GO:0030246; GO:0097173
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00570217}.; SIMILARITY: Contains 1 SIS domain. {ECO:0000256|HAMAP-Rule:MF_00068}.
Protein families: GCKR-like family, MurNAc-6-P etherase subfamily
Coiled coil:
Domain [FT]: DOMAIN 58 222 SIS. {ECO:0000259|PROSITE:PS51464}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92589
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00068
PANTHER: IPR005488;IPR005486;IPR001347
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01272;PS51464
ProDom: PF01380
SMART:
SUPFAM:
TIGRFAMs:

102827-103733(-)

>nucleotide sequence
TTATTCATTAATTATTTTACTAACTAAATTATCATATTTAGTTAGTAATGTCTGACTTTC
AACATAAGAAACATTTTTTAAAATCATTACAATTGCATTTTTACAAGCATAATTCGCTGC
TGTTAATGCCTGCGCAATAACCTCATCACTAGCATTTGTAACAGCTTTAACAATTTCTGC
TGTTCGTACTTTTAATTTTTCATTAGTTGCAACTAAATCAACCATTAAATTTTGATATAC
TTTTCCTAATTTAACCATTAATGTTGTTGAAATCATATTGCATACTAATTTTGTTGCTGT
TCCCGCTTTCATTCTAGTACTGCCAGTAATAACTTCTGGTCCTGTTTCAATTGCAATTAC
TTCATCAGCAATTGCTATCATTTCCGAGTTTTTTGTCATACACAAACCAACTGATAATGC
ACCAATTGTTTTAGCATATTTTAATCCTGCTAAAACATAGGGTGTTCGCCCAGAAGCACC
AATCCCAATTACAGTATCTAATAACGGTTGTAAATTTAATTTCTTTAAGTCATTTACCGC
TAAATCTTGGTCATCTTCCGCACCTTCCGCTGGTGTGCGAATAGCACTATCACCACCGGC
AATAATCCCAACAATACGGTCAGCATCAAGTCCATACGTTGGTAACATCTCTGATGCATC
AAGAATCCCAATTCGTCCCGATGATCCTGCTCCCATATAAATTAATCGACCCCCTTGGTT
AAAACGCGGAAAAATTAAATCAATTACTTTAGTAATAACGGGTATTTTTGCTTGCACTGC
TTGCGCAATTTTTGCATCTTCATTATTAATAATTGTTAAAATACCCTTTGTATCTGTTTG
ATCAATATGAGTACTATTTGGATTACGTTGTTCTGTGTCTATTTTTGACAAATTAATTTT
ATTCAT

>protein sequence
MNKINLSKIDTEQRNPNSTHIDQTDTKGILTIINNEDAKIAQAVQAKIPVITKVIDLIFP
RFNQGGRLIYMGAGSSGRIGILDASEMLPTYGLDADRIVGIIAGGDSAIRTPAEGAEDDQ
DLAVNDLKKLNLQPLLDTVIGIGASGRTPYVLAGLKYAKTIGALSVGLCMTKNSEMIAIA
DEVIAIETGPEVITGSTRMKAGTATKLVCNMISTTLMVKLGKVYQNLMVDLVATNEKLKV
RTAEIVKAVTNASDEVIAQALTAANYACKNAIVMILKNVSYVESQTLLTKYDNLVSKIIN
E