SPM_000725

Uniprot: A0A037UMH8
Description: glycerol kinase
EC number: 2.7.1.30
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3-phosphate. {ECO:0000256|HAMAP-Rule:MF_00186, ECO:0000256|SAAS:SAAS00029455}.
Cofactor:
Enzyme regulation: ENZYME REGULATION: Inhibited by fructose 1,6-bisphosphate (FBP). {ECO:0000256|HAMAP-Rule:MF_00186}.
Function [CC]: FUNCTION: Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. {ECO:0000256|HAMAP-Rule:MF_00186, ECO:0000256|SAAS:SAAS00408880}.
Pathway: PATHWAY: Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. {ECO:0000256|HAMAP-Rule:MF_00186, ECO:0000256|SAAS:SAAS00029305}.
Active site:
Binding site: BINDING 12 12 Substrate. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 16 16 ATP. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 134 134 Substrate. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 269 269 ATP. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 312 312 ATP; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 316 316 ATP; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00186}.; BINDING 331 331 ATP. {ECO:0000256|HAMAP-Rule:MF_00186}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 12 14 ATP. {ECO:0000256|HAMAP-Rule:MF_00186}.; NP_BIND 413 417 ATP. {ECO:0000256|HAMAP-Rule:MF_00186}.
Site:
Gene names (primary): glpK
Gene names (synonym):
Mass: 56,310
Subunit structure [CC]:
Gene ontology (GO): ATP binding [GO:0005524]; glycerol kinase activity [GO:0004370]; glycerol catabolic process [GO:0019563]; glycerol-3-phosphate metabolic process [GO:0006072]
Gene ontology IDs: GO:0004370; GO:0005524; GO:0006072; GO:0019563
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the FGGY kinase family. {ECO:0000256|HAMAP-Rule:MF_00186, ECO:0000256|RuleBase:RU003733, ECO:0000256|SAAS:SAAS00546148}.
Protein families: FGGY kinase family
Coiled coil:
Domain [FT]: DOMAIN 4 254 FGGY_N. {ECO:0000259|Pfam:PF00370}.; DOMAIN 264 452 FGGY_C. {ECO:0000259|Pfam:PF02782}.
Motif:
Region: REGION 82 83 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00186}.; REGION 247 248 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00186}.
EMBL: AGBZ02000001
ProteinModelPortal: A0A037UMH8
MEROPS:
EnsemblBacteria KO: KAI92627
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00186
PANTHER: IPR000577;IPR018485;IPR018483;IPR018484;IPR005999
PIRSF:
PRINTS: PIRSF000538
PROSITE:
Pfam: PS00933;PS00445
ProDom: PF02782;PF00370
SMART:
SUPFAM:
TIGRFAMs:

141348-142860(+)

>nucleotide sequence
ATGTCAAAATATATTATCAGTCTTGATGAAGGAACCACAAGCTGTCGTACTCTTGTTATT
AATCAACAAGGTGAAATCATTTCTAGTGATGCCTTAGAATTTAGCCAAATTTTTCCTAGA
GCTGGTTGAGTTGAACATGATGCTGTTGAAATTTGAAACTCGCAACGAACAACTCTTGTT
CAATCGTTAAATAAAGCAAAAATAAAACCAAGTGAAATTGCCGGAATTGGTATTACCAAT
CAACGTGAAACAGTAGTTATGTGAGATAAACGCTCAGGATTACCAATTTATAATGCTATT
GTCTGACAAGATCGTAGAACAGCCGCTTATTGTGATGAATTAATTGCGGAAGGAAAAAGT
GATCTTATTCAAGCAAAAACTGGTTTAGTAATTAACCCATATTTTTCAGCAACAAAAATC
AAATGAATTTTAGACAATGTTGAAGGTGCACGCCAATTAGCAAAAGAAAATAATCTTTTA
TTTGGGACAATTGATACTTGACTAATTTATCGTCTAACAGGAGGTAAAATTCATATCACT
GACTCAACTAATGCTAGTCGGACAATGCTATTCAATATTCATACTAAACAATGAGATGAT
GAACTATTAACTTTACTAGATATTCCAAAAAATATTTTACCAATGGTAAAATCATCATCA
GAAATTTATGGTTATACTTTCCCCGGTTTATTAAGTAAAGAAACTGATATCCAAATTCCA
ATTGCAAGTGCTATTGGTGACCAACAAGCTGCTTTATTTGGTCAATTATGTTTAGAACCA
GGGGAAGTTAAAAACACTTATGGAACGGGATGTTTTATTTTAATGAATACTGGTGAAAAA
CCAGTTACATCAAAAAATGGTTTATTAACAACCATTGCGCTAGATTATCAAAACAAAATT
ACTTATGCCTTAGAAGGGTCAGTCTTTGTTGCTGGCGCTGCTATTCAATGACTTCGTGAT
CAAATTAAAATTGTTTATCATGCTAGTGAAACAGATTGATATACTAATCTTGTTCAAGAT
GACCAGCAAGTTTATGTGGTTCCTTCCTTTACCGGGTTAGGATCACCTTACTGAGATTCT
TATTCACGGGGAGCAATTTTTGGATTAGAACGAGGAACAAAGCGCGAACATTTAGTTAAA
GCAACATTAGAATCACTAGCTTACCAATCATATGATGTTATTACTGCAATGGCTGAAGAT
TTACAACAACCAATTAAACGAATTAAAGTTGATGGTGGCGCTAGTCGGAATGAATATTTA
ATGCAATTCCAAGCTGATATTTCACAAGTAGAAGTAATTCGACCTCAAAATATTGAAACA
ACAGCTATGGGAGCTGCTTTTCTAGCCGGATTAGCTGTTGGATTCTGAACATCACCAACA
GAAATTAAAAAAATATTAGTAGTTGGAAAAAAATATCATGCTAAATTAAAACAAGAAATT
GTTACAAAATTATTAAAAGGGTGAAAAGTTGCTGTTAAGCGTACTTTAAACTGATTAAAA
GATATTGAATAA

>protein sequence
MSKYIISLDEGTTSCRTLVINQQGEIISSDALEFSQIFPRAGWVEHDAVEIWNSQRTTLV
QSLNKAKIKPSEIAGIGITNQRETVVMWDKRSGLPIYNAIVWQDRRTAAYCDELIAEGKS
DLIQAKTGLVINPYFSATKIKWILDNVEGARQLAKENNLLFGTIDTWLIYRLTGGKIHIT
DSTNASRTMLFNIHTKQWDDELLTLLDIPKNILPMVKSSSEIYGYTFPGLLSKETDIQIP
IASAIGDQQAALFGQLCLEPGEVKNTYGTGCFILMNTGEKPVTSKNGLLTTIALDYQNKI
TYALEGSVFVAGAAIQWLRDQIKIVYHASETDWYTNLVQDDQQVYVVPSFTGLGSPYWDS
YSRGAIFGLERGTKREHLVKATLESLAYQSYDVITAMAEDLQQPIKRIKVDGGASRNEYL
MQFQADISQVEVIRPQNIETTAMGAAFLAGLAVGFWTSPTEIKKILVVGKKYHAKLKQEI
VTKLLKGWKVAVKRTLNWLKDIE