SPM_000890

Uniprot: A0A037UQ02
Description: acetate kinase
EC number: 2.7.2.1
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + acetate = ADP + acetyl phosphate. {ECO:0000256|HAMAP-Rule:MF_00020}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00020}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|HAMAP-Rule:MF_00020}; ; Note=Mg(2+). Can also accept Mn(2+). {ECO:0000256|HAMAP-Rule:MF_00020}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. {ECO:0000256|HAMAP-Rule:MF_00020}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. {ECO:0000256|HAMAP-Rule:MF_00020}.
Active site: ACT_SITE 145 145 Proton donor/acceptor. {ECO:0000256|HAMAP-Rule:MF_00020}.
Binding site: BINDING 13 13 ATP. {ECO:0000256|HAMAP-Rule:MF_00020}.; BINDING 89 89 Substrate. {ECO:0000256|HAMAP-Rule:MF_00020}.
Calcium binding:
DNA binding:
Metal binding: METAL 6 6 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00020}.; METAL 382 382 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00020}.
Nucleotide binding: NP_BIND 205 209 ATP. {ECO:0000256|HAMAP-Rule:MF_00020}.; NP_BIND 280 282 ATP. {ECO:0000256|HAMAP-Rule:MF_00020}.; NP_BIND 328 332 ATP. {ECO:0000256|HAMAP-Rule:MF_00020}.
Site: SITE 177 177 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00020}.; SITE 238 238 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00020}.
Gene names (primary): ackA
Gene names (synonym):
Mass: 44,094
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00020}.
Gene ontology (GO): cytoplasm [GO:0005737]; acetate kinase activity [GO:0008776]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; acetyl-CoA biosynthetic process [GO:0006085]; organic acid metabolic process [GO:0006082]
Gene ontology IDs: GO:0000287; GO:0005524; GO:0005737; GO:0006082; GO:0006085; GO:0008776
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the acetokinase family. {ECO:0000256|HAMAP-Rule:MF_00020, ECO:0000256|RuleBase:RU003835, ECO:0000256|SAAS:SAAS00589723}.
Protein families: Acetokinase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92641
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00020
PANTHER: IPR004372;IPR000890;IPR023865
PIRSF: PTHR21060
PRINTS: PIRSF000722
PROSITE: PR00471
Pfam: PS01075;PS01076
ProDom: PF00871
SMART:
SUPFAM:
TIGRFAMs:

166796-167987(-)

>nucleotide sequence
TTATGAATTAATTAAATGATAAGTATCTTCACAAATCATTACTTCTTCATTTGTTCGCAT
CGCATAAATAAGAACATCTGATTTTGATGTTGAAATTAGATTTTCTGTGTCATATGAACT
TTGATTTGCTGAAGGAGATAATTCTAAGTTCAATAAAGTAACTTCATTAACGACTAATTG
GCGCATTTCATCAGAATTTTCACCAATCCCAGCGGTAAAAACAATTGCATCTGGTTTTAT
TTTTAAATCATTAGCATAACTAACAATATATTTTGCAATTCTTTTTGCACTCATTAGCAA
CGCTAATTTACTACGTTGATTATCCTTGCTACTTTTAAAAACATCACGTAAATCAGATGA
TACTCCCGAAATTCCTAATAAACCAGATTCTTTATTTAAAGTATTAGTTATTTCATCTAA
TGTTGTTCCAATTTGATTTGCAATAAATTGGTGAATTGAGGGGTCAATATCACCACTTCG
TGTTCCCATAATTAATCCTTCTAATGGCGTTAAGCCCATTGAAGTATTAAAACTTTTTCC
ATTTTTAACTGCACAAATTGAAGCACCATTGCCTAAATGACAAATAATTGCATTAAGTTC
AGTTGCTGGTTTTTGTAAAACTTCAGCTAATCGTTTTGTAATATAACGATAAGAAATGCC
ATGAAAACCATAACGGCGAACTTGATAATTACTATATCATTCATAAGGAACTGAATATAA
ATAATTTTCCTCAGGAATTGTTGTATGGAATGAAGTATCAAATACTGCAACATTTGGTAC
ATCAACAATCTTACGAAAAGCTTCAATTGCGGCAATTGAAGGCGGATTATGCAGCGGAGC
TAATGTTACCATTCGTTTAATTTCGGCAAATACTTCATCGTTAATAACTATTGATTGTGT
AAACTTTTCACCACCATGCACAATTCGATGGCCAATTCCTTGAATATCAGCAAAATCTTG
AATAATATGATGTTCTTTTAATTTAGCGATTAAAACCTTTGCTGCTGCTTTATGATCTGG
AAAATCTTCTTTTGTTTGAAACTCCTGACCATTAAATTTAAGGGTAAAAATTCCATCAAT
GTTAATTCGTTCAGCAAGTCCTTTACAAATTACTTCATAATTTTTTGCTGAATTAACTTT
ATATAATTGAAATTTCATCGAGCTACTACCAGCATTTATTACTAAAATCAT

>protein sequence
MILVINAGSSSMKFQLYKVNSAKNYEVICKGLAERINIDGIFTLKFNGQEFQTKEDFPDH
KAAAKVLIAKLKEHHIIQDFADIQGIGHRIVHGGEKFTQSIVINDEVFAEIKRMVTLAPL
HNPPSIAAIEAFRKIVDVPNVAVFDTSFHTTIPEENYLYSVPYEWYSNYQVRRYGFHGIS
YRYITKRLAEVLQKPATELNAIICHLGNGASICAVKNGKSFNTSMGLTPLEGLIMGTRSG
DIDPSIHQFIANQIGTTLDEITNTLNKESGLLGISGVSSDLRDVFKSSKDNQRSKLALLM
SAKRIAKYIVSYANDLKIKPDAIVFTAGIGENSDEMRQLVVNEVTLLNLELSPSANQSSY
DTENLISTSKSDVLIYAMRTNEEVMICEDTYHLINS