SPM_001115

Uniprot: A0A037UQ47
Description: translation initiation factor IF-2
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. {ECO:0000256|HAMAP-Rule:MF_00100, ECO:0000256|RuleBase:RU000644}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 127 134 GTP. {ECO:0000256|HAMAP-Rule:MF_00100}.; NP_BIND 174 178 GTP. {ECO:0000256|HAMAP-Rule:MF_00100}.; NP_BIND 228 231 GTP. {ECO:0000256|HAMAP-Rule:MF_00100}.
Site:
Gene names (primary): infB
Gene names (synonym):
Mass: 68,044
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; translation initiation factor activity [GO:0003743]
Gene ontology IDs: GO:0003743; GO:0003924; GO:0005525; GO:0005737
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. {ECO:0000256|HAMAP-Rule:MF_00100, ECO:0000256|RuleBase:RU000644}.
Protein families: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily
Coiled coil:
Domain [FT]: DOMAIN 118 288 Tr-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51722}.
Motif:
Region: REGION 121 270 G-domain. {ECO:0000256|HAMAP-Rule:MF_00100}.
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92671
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.10050;3.40.50.300
InterPro: MF_00100_B
PANTHER: IPR006847;IPR027417;IPR005225;IPR000795;IPR000178;IPR023115;IPR009000
PIRSF:
PRINTS:
PROSITE: PR00315
Pfam: PS51722
ProDom: PF11987;PF04760
SMART:
SUPFAM:
TIGRFAMs: SSF50447;SSF52156;SSF52540

201870-203724(-)

>nucleotide sequence
TTACTTTGTAACGGCTTTCTCAGTATAAGCTTCAATAACATCTTGCTCTTTTAAATCATT
ATAGTTTTTAATTGTTAGGCCACATTCAACTCCTGTTTTAGCTTCTTTAATATCATCTTT
AATATGTTTCAATGATGCTAATTCACCACTATAGACAATAACCCCATCGCGTAAAACGCG
AACTCGTGATTTTCGTGGAATAATACCATCAGTAACATAACAACCAGCAATCGTTCCAAT
ATCAGAATGGCGGAAAATTTGACGTACTTCCGCTTGCCCTAACACTTCTTCAACCATTTC
TGGATCTAACATTCCTTCTGCTGCTGCAACTATTTCTTCCGTTAATTTATAAATAATGGT
ATGCAAACGAATTGCAACTCCTTCATCTTCGGCTTTTTTTCGAACATGCGCAGTTGGACG
AACATTAAAACCAACAATAAATGCTTGACTAGCTAATCCTAATGTTACATCACTTTCGGA
AATTCCCCCAACAGTTGCTCGTAAAACATTAATTTTTACGCCTGCAATATTAATTTTTAA
TAAACTTGCTTTTACCGCTTCAACTGTTCCTTGAGTATCAGCTTTTAAAATAATATTAAT
TTGCTTTAACTGCCCATCTTTAATTTGTGCTGATAGACTTTCTAATGAAAAATTTTGATT
TTTATAACGTTTATTCATTGTATCAATTGTTTTTCGTTTTAAGGCAATTTCACGTGCTAA
TTTTTCATCCCGGAAAACCATAAAACGATCACCAGCATTTGGTACTTCATTTAAACCATG
AATCATAACTGGAGTTGATGGTCCTGCTGATTTAATTTTTTTACCACTTTCATCAGACAA
ATCACGAACATAACCAAAAGTATAACCAACAACTAAGGCATCTTTAATATTCAATGTTCC
TGATTGCACCAATAATGTTGCAACTGGTCCTAAACCACGATTTAAGTGTGATTCAAGCAC
TGTACCTAACGCAAAGCGATTTGGATTTGCTTTTAATTCCATCAAATCAGCCATTAATAA
GATTGTATCTAATAATTCATCAATTCCTTGCTTTTGTTTAGCACTTCCTTTAACATAAGG
GGTTGTGCCTCCTCATTCTTCTGGAGTTAATTCTTCTTGTGATAATTGCATTAAAATATT
ATCTAAATTTATCCCAACTTTATCCATTTTATTAACAAAAACAATAATTGGTACGCCAGC
TGCTTTGGCATGATCAATTGCTTCTTTAGTTTGCAACATTACCCCATCGTCAGCTGCAAC
AACTAGGACAACAATATCTGTTACCGCACTCCCTCGTGCTCGCATTTGTGTAAAAGCTTC
ATGTCCAGGAGTATCAACAAATGTAATCTTTTCATTATTTTTAGTTTTAATTTGATAAGC
TCCAATATGTTGTGTAATTCCCCCATGTTCTGTACTTACAACATTAGTATTTCGAATGGT
ATCTAATAATGTTGTTTTGCCATGATCAACATGGCCCATAATTGTAACAATTGGCGGACG
CTTTTCCAACGAAGCAGGGTCATCATTAACTTCAAAACTTTCAATTAAGTTTTCATGCGT
TACTGATTTTTCTCGTTTAAAATCTAACCCAAATTCTAAACATAACTCTCCCAATTGATC
TTCAGTTAAAAAAGTATTTTGATTTAACATTATTCCCTTACTAAAAAAATATTTAATAAT
TTCACTAACGGGTTTATTAATTTTTTGTGCAAATTCAGCAATGGTTAATGCCTCATCATA
AACAAAAACTCCCTCCGTAACATGTGCTTCAATCGTTTTTAATTGCGATTGAATTCGATT
TCGTTGTTTTTTAGCTAGTGTTTTTTTATTTTGTTTGTTATTTTGACTACTCAT

>protein sequence
MSSQNNKQNKKTLAKKQRNRIQSQLKTIEAHVTEGVFVYDEALTIAEFAQKINKPVSEII
KYFFSKGIMLNQNTFLTEDQLGELCLEFGLDFKREKSVTHENLIESFEVNDDPASLEKRP
PIVTIMGHVDHGKTTLLDTIRNTNVVSTEHGGITQHIGAYQIKTKNNEKITFVDTPGHEA
FTQMRARGSAVTDIVVLVVAADDGVMLQTKEAIDHAKAAGVPIIVFVNKMDKVGINLDNI
LMQLSQEELTPEEWGGTTPYVKGSAKQKQGIDELLDTILLMADLMELKANPNRFALGTVL
ESHLNRGLGPVATLLVQSGTLNIKDALVVGYTFGYVRDLSDESGKKIKSAGPSTPVMIHG
LNEVPNAGDRFMVFRDEKLAREIALKRKTIDTMNKRYKNQNFSLESLSAQIKDGQLKQIN
IILKADTQGTVEAVKASLLKINIAGVKINVLRATVGGISESDVTLGLASQAFIVGFNVRP
TAHVRKKAEDEGVAIRLHTIIYKLTEEIVAAAEGMLDPEMVEEVLGQAEVRQIFRHSDIG
TIAGCYVTDGIIPRKSRVRVLRDGVIVYSGELASLKHIKDDIKEAKTGVECGLTIKNYND
LKEQDVIEAYTEKAVTK