SPM_001405

Uniprot: A0A037USW6
Description: ribonuclease III
EC number: 3.1.26.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231310}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231322};
Enzyme regulation:
Function [CC]: FUNCTION: Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. {ECO:0000256|HAMAP-Rule:MF_00104}.
Pathway:
Active site: ACT_SITE 47 47 {ECO:0000256|HAMAP-Rule:MF_00104}.; ACT_SITE 119 119 {ECO:0000256|HAMAP-Rule:MF_00104}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 43 43 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 116 116 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 119 119 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.
Nucleotide binding:
Site:
Gene names (primary): rnc
Gene names (synonym):
Mass: 27,464
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231419}.
Gene ontology (GO): cytoplasm [GO:0005737]; metal ion binding [GO:0046872]; ribonuclease III activity [GO:0004525]; rRNA binding [GO:0019843]; mRNA processing [GO:0006397]; rRNA catabolic process [GO:0016075]; rRNA processing [GO:0006364]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0004525; GO:0005737; GO:0006364; GO:0006397; GO:0008033; GO:0016075; GO:0019843; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain. {ECO:0000256|HAMAP-Rule:MF_00104}.; SIMILARITY: Contains 1 RNase III domain. {ECO:0000256|HAMAP-Rule:MF_00104}.
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 3 130 RNase III. {ECO:0000259|PROSITE:PS50142}.; DOMAIN 157 228 DRBM (double-stranded RNA-binding). {ECO:0000259|PROSITE:PS50137}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92715
UniPathway:
CDD:
Gene3D: cd00593
HAMAP: 1.10.1520.10;3.30.160.20
InterPro: MF_00104
PANTHER: IPR014720;IPR011907;IPR000999
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50137;PS00517;PS50142
ProDom: PF00035;PF14622
SMART:
SUPFAM: SM00358;SM00535
TIGRFAMs: SSF69065

255834-256545(-)

>nucleotide sequence
TTATTTTTCGAAATGATATTTTGATTTTGTTGCTAATTTTTGTAATGCATCACGAGCTGC
TAATTGCTCAGCTTCTTTATGAGTATAACCGCATCCTTCACCATACCGAATCCCCCCAAT
TTCTGCAACCACAGTATATAAAACTCGATTTCCTTCTAATGGTGGTGATTCTTTAATCAA
TTTATATTCTAATGTTCGAGCATCACCAGCCTGTAAAAATTCTTGTAATTCAGTTTTGTA
ATCTCGAATTTGGTCAAAAAAACCAGGATTAGAAACCATTCTAATAAGAGTATTATTAAT
AAAAGTACGAACGACCTCTTCACCTAAATCAAGATATAATGCAGCAATCATTGATTCATA
AATATCAGCTAAAATACGATTTTTTTCATATCCTTTTGTTTTAATTTCTCCTTTTCCTAA
TAATAAGAAAGGTGCTCAATTCATTCGACGAACTAATTCTGCTAATGTTCCTTCACGAAC
AATTTTACTACGTAAATTAGTAATTTCACCTTCATTTTTATCAGGAAAAATTAAAAATAA
GTACAAGGAAATTTCTTTTGCTAAAATTGCATCGCCCAAAAATTCCATTCGTTGATAAGT
ATAACTTAAATTATGCTCATTTGCATAAGAATTATGGGTTAATGCTTCTAAATAATATTG
TCGTTCTTTAATAAAAACATGATATTGTTTAAAAAAAGCTTTTAATTCAAT

>protein sequence
IELKAFFKQYHVFIKERQYYLEALTHNSYANEHNLSYTYQRMEFLGDAILAKEISLYLFL
IFPDKNEGEITNLRSKIVREGTLAELVRRMNWAPFLLLGKGEIKTKGYEKNRILADIYES
MIAALYLDLGEEVVRTFINNTLIRMVSNPGFFDQIRDYKTELQEFLQAGDARTLEYKLIK
ESPPLEGNRVLYTVVAEIGGIRYGEGCGYTHKEAEQLAARDALQKLATKSKYHFEK