SPM_001440

Uniprot: A0A037UQB7
Description: molecular chaperone GrpE (heat shock protein)
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. {ECO:0000256|HAMAP-Rule:MF_01151, ECO:0000256|RuleBase:RU000639, ECO:0000256|SAAS:SAAS00067045}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): grpE
Gene names (synonym):
Mass: 23,479
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01151, ECO:0000256|SAAS:SAAS00066998}.
Gene ontology (GO): cytoplasm [GO:0005737]; adenyl-nucleotide exchange factor activity [GO:0000774]; protein folding [GO:0006457]
Gene ontology IDs: GO:0000774; GO:0005737; GO:0006457
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GrpE family. {ECO:0000256|HAMAP-Rule:MF_01151, ECO:0000256|RuleBase:RU004478, ECO:0000256|SAAS:SAAS00562011}.
Protein families: GrpE family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92721
UniPathway:
CDD:
Gene3D: cd00446
HAMAP: 2.30.22.10;3.90.20.20
InterPro: MF_01151
PANTHER: IPR000740;IPR013805;IPR009012
PIRSF: PTHR21237
PRINTS:
PROSITE: PR00773
Pfam: PS01071
ProDom: PF01025
SMART:
SUPFAM:
TIGRFAMs: SSF51064

263275-263899(-)

>nucleotide sequence
TTATTTTGCCACTTGAACTGATGCATGCCGTAAAACACGATCATGAATCATATATCCTTT
TTGTAAAATTTTAACAATACATCCTGATGAAACATCAGTTGTTTCAATAATTTCAATTGC
CGAATGGAAATTAGAATTGAAGTGTTCTCCTACTTTTGTTTCCATTGCGGTAATACCTTC
TTCTTCAAAAACCGTTTTAAACATACGATAAATCATTTCAAAACCTGTTAAAAAGTTTTT
CACTTCTGGTGTTACATTAGTAACTTGCAAAGCACGTTCAAAGTTATCTAATGTTGGCAA
TAATTTTTCTGCCATTCCTGCTGCCCGATAACGTTTAATATCAGCAACTTCTTCATGAAT
TCTTTTCTTTAAATTTTCACCATCAGCTAAAGCTAATAATTTTTCTTCTCGCAATCGTAA
ATTGTCTTTTAACAATAAATCAATTTCATGTTCTAAATCTTCAATAATCGCCAAATTATT
TGCTTCAGCTGTCGGCGCTGTTGCTGTTTTTGATGATTCTGACAGTGAATCAACAGGTTG
ATTATTTGCTAATTCTTTCCGTAATTGTTGAATTTTTTCTTTTAATTGGTTTGGCGAAGA
TTTATCATTTTTTTCATTACTCAT

>protein sequence
MSNEKNDKSSPNQLKEKIQQLRKELANNQPVDSLSESSKTATAPTAEANNLAIIEDLEHE
IDLLLKDNLRLREEKLLALADGENLKKRIHEEVADIKRYRAAGMAEKLLPTLDNFERALQ
VTNVTPEVKNFLTGFEMIYRMFKTVFEEEGITAMETKVGEHFNSNFHSAIEIIETTDVSS
GCIVKILQKGYMIHDRVLRHASVQVAK