SPM_001790

Uniprot: A0A037ULQ9
Description: tyrosine--tRNA ligase
EC number: 6.1.1.1
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00105588}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00009764}.
Pathway:
Active site:
Binding site: BINDING 35 35 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 165 165 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 169 169 Tyrosine. {ECO:0000256|HAMAP-Rule:MF_02006}.; BINDING 230 230 ATP. {ECO:0000256|HAMAP-Rule:MF_02006}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): tyrS
Gene names (synonym):
Mass: 47,973
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00009771}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; RNA binding [GO:0003723]; tyrosine-tRNA ligase activity [GO:0004831]; tyrosyl-tRNA aminoacylation [GO:0006437]
Gene ontology IDs: GO:0003723; GO:0004831; GO:0005524; GO:0005737; GO:0006437
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_02006, ECO:0000256|SAAS:SAAS00538763}.; SIMILARITY: Contains 1 S4 RNA-binding domain. {ECO:0000256|HAMAP-Rule:MF_02006}.
Protein families: Class-I aminoacyl-tRNA synthetase family, TyrS type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 349 415 S4 RNA-binding. {ECO:0000259|PROSITE:PS50889}.
Motif: MOTIF 40 49 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_02006}.; MOTIF 227 231 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_02006}.
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92769
UniPathway:
CDD:
Gene3D:
HAMAP: 3.10.290.10;3.40.50.620
InterPro: MF_02006
PANTHER: IPR001412;IPR002305;IPR014729;IPR002942;IPR002307;IPR024088;IPR024107
PIRSF: PTHR11766
PRINTS:
PROSITE: PR01040
Pfam: PS00178;PS50889
ProDom: PF00579
SMART:
SUPFAM: SM00363
TIGRFAMs:

327456-328719(-)

>nucleotide sequence
TTATTTTTTGTTAGTTGTAAGATAAATTAAATGATATTTTCGTTTTCCACGACGAATAAT
TAAATATTTATTAAATAAAAACTTATCTTTTGCTACAGTTCAAGTTTCTTCTTTAATAAT
TTGACCATTAATAGTAATTGCACCTTGTTCTAAAAACTCACGACCTTCACGTTTAGAAGT
AACAATTTCTGCTTGTATTAATAAATCAAGAAGTGGTAAATTAAAGTTTGTTAAATGAAA
ATTAGGTAAACTGTTATGAAGTTGCTCTAACTGTTGTTCTGATAATTCATGTAAACTGCC
TTTAAATAAGGCTTCACTAACAATTTGTGCTGTTTTTAGTCCTGCTGCTTGATGAACAAA
TAAAGTTACTTCTTCTGCTAATTTTTTTTGTACAATGCGTTGTGCTGGTTCATTTTGGTG
CGCTGTTTCAAGGGCTATAATTTCTTTTTCAGATAACATTGTTAAATAACGCAACAATTT
TGCTGTTTCTTGGTCATCTTGATTATAAAAGAATTGATAAAATTCATAGGGTGATGTTTT
TTTTGAATCTAATCAAACTGCCCCTGATTCTGTTTTTCCAAATTTTGAACCATCAGCTTT
TGTTAATAAATTCATTGTCAAACCACAAGCTAAATTATCTTCTCCAATTTGTTTATAAAT
ATAATCTGTTCCGGAAGTAATATTGCCTCATTGGTCACTACCACCAGTCTGAACAGCACA
ATCATAATTAATATATAATTGATAAAAGTCATAAGCCTGTAATAAAGTGTAAGCAAATTC
AGTATATGATAAACCAACTTCAATTCTAGTTGCAATATTTTCTTTTGCTAATAAATAACT
AATATTAAAATCTTTGCCAACATTTCGCAAAAAATCAATCAAGGTCATTTTTTGTAATCA
TTCAGCATTATTAACCATTTTAATTTTTACACCAATTAATTGGTTCATTTGTTGACTAAT
AGTTTCAACATTATGTTTAATTGTTTTATCATCTAACAATACTCGTTCTGCTTTTTTCCC
ACTAGGATCACCAATCATTCCTGTTCCACCACCAATAATAGCAATTGGTTGAAATCCAAA
CATTTCAAAACGTTTTAATAACATAATTTGAATTAAATGACCAACATGTAACGAATCACC
GGTTGGATCAAAACCACAATAAACTCCTTTTTTTAATTCTTGCGCTTTTAATAATTTTGT
TTCATTAGTTACTTGCTTTAATAGTCCTCTTCATCTTAATTCTTCTAAAATATTTTTTAC
CAT

>protein sequence
MVKNILEELRWRGLLKQVTNETKLLKAQELKKGVYCGFDPTGDSLHVGHLIQIMLLKRFE
MFGFQPIAIIGGGTGMIGDPSGKKAERVLLDDKTIKHNVETISQQMNQLIGVKIKMVNNA
EWLQKMTLIDFLRNVGKDFNISYLLAKENIATRIEVGLSYTEFAYTLLQAYDFYQLYINY
DCAVQTGGSDQWGNITSGTDYIYKQIGEDNLACGLTMNLLTKADGSKFGKTESGAVWLDS
KKTSPYEFYQFFYNQDDQETAKLLRYLTMLSEKEIIALETAHQNEPAQRIVQKKLAEEVT
LFVHQAAGLKTAQIVSEALFKGSLHELSEQQLEQLHNSLPNFHLTNFNLPLLDLLIQAEI
VTSKREGREFLEQGAITINGQIIKEETWTVAKDKFLFNKYLIIRRGKRKYHLIYLTTNKK