SPM_002505

Uniprot: A0A037UQT6
Description: DNA gyrase subunit A
EC number: 5.99.1.3
Annotation score: 3 out of 5
Miscellaneous [CC]: MISCELLANEOUS: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes. {ECO:0000256|HAMAP-Rule:MF_01897}.
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000256|HAMAP-Rule:MF_01897, ECO:0000256|SAAS:SAAS00075911}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. {ECO:0000256|HAMAP-Rule:MF_01897}.
Pathway:
Active site: ACT_SITE 128 128 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000256|HAMAP-Rule:MF_01897}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gyrA
Gene names (synonym):
Mass: 92,477
Subunit structure [CC]: SUBUNIT: Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis. {ECO:0000256|HAMAP-Rule:MF_01897}.
Gene ontology (GO): chromosome [GO:0005694]; cytoplasm [GO:0005737]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (ATP-hydrolyzing) activity [GO:0003918]; DNA topological change [GO:0006265]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0003677; GO:0003918; GO:0005524; GO:0005694; GO:0005737; GO:0006261; GO:0006265
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the topoisomerase GyrA/ParC subunit family. {ECO:0000256|HAMAP-Rule:MF_01897}.; SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. {ECO:0000256|SAAS:SAAS00565436}.
Protein families: Topoisomerase GyrA/ParC subunit family; Type II topoisomerase GyrA/ParC subunit family
Coiled coil: COILED 441 482 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 17 469 TOP4c. {ECO:0000259|SMART:SM00434}.
Motif: MOTIF 531 537 GyrA-box. {ECO:0000256|HAMAP-Rule:MF_01897}.
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92886
UniPathway:
CDD:
Gene3D:
HAMAP: 3.30.1360.40;3.90.199.10
InterPro: MF_01897
PANTHER: IPR024946;IPR005743;IPR006691;IPR013760;IPR002205;IPR013758
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF03989;PF00521
SMART:
SUPFAM: SM00434
TIGRFAMs: SSF56719

469950-472413(+)

>nucleotide sequence
ATGAAATCAGAAAATGATGGATATGATTATGATGGTAAAATTCGTGATATTGATATTGCT
GATGAAATGAAAAATGGTTTTTTAGATTATGCAATGTCAGTTATTGTTTCACGTGCAATT
CCTGATGTTCGTGATGGATTAAAACCAGTTCATCGACGCATTATTTATGCAATGTGAGAT
TTAAAAATGACTTACGAAAAACAACACAAGAAATCAGCACGAATTGTTGGTGAAGTAATT
GGGAAATATCATCCCCATGGTGATACTGCTGTTTATGAAGCAATGGTTCGGATGGCACAA
GATTTTTCTTATCGTTATCCTTTAATTGATGGTCATGGAAATTTTGGTTCAATGGATGGT
GACGCTCCAGCGGCAATGCGATACACGGAAGCAAAAATGAGTAAAATTGCTGGTGAACTA
ATCAAAGATATTGAAAAAGAAACAACAATTTTTATTGATAATTATGATGGTAGTGAAGAA
GAACCGACTTTTTTACCAGGATATTTTCCAAATTTATTAGTTAATGGTGCTAGTGGAATT
GCTGTTGGAATGGCAACTAATATTCCACCCCATAATTTGAATGAAGTAATTGATGGAGTT
ATTGCGGTAACAAAAAATCCTGAAATAACGACCGTTGAATTAATGAAAATTATTAAAGGA
CCTGATTTTCCAACTGGGGCATTAATTACGGCTGGAAACGGTTTAATTAAAGCTTATGAA
ACAGGTAATGGAGCAATTACAATTCGTTCAAAGATTGATATTGAAGAAAATAATAATAAA
AGACGAATTATTGTGTCAGAGATTCCATATCAAATTAATAAAGCAAAATTGTTGGAACGA
ATTGCAGAATTAGTTCGCGATAAAATTATTAATGGAATTAGTGACCTTCGTGATGAATCA
AACATGGAGGGGGTTCGTGTTGTTTTAGAATTAAAACGTGATGCTCAAGAAAATGTTATT
TTAAATAAATTATATAAATTAACGCCACTCCAAACTAATTTTTCGTTAAATATTTTAGCA
TTGTACAAAAACCAACCAAGAGTAATGGGATTAAAACAAATTTTAAAATATTTTATTGAA
CATCAAATTGATATTATTATTAAACGTAGTCAATTTGAATTAAAGAAAAATCGTAATCGT
TTAATCATTTTAGAAGGATTAAAAATTGCTCTTGATAATATTGATGAAGTAATTGCAATT
ATTAAACAATCACAAACAACACAAATTGCATCAGATGCCTTGATTAATAAATTTAATTTA
GTAGGTGAACAAGCACGGGCAATTTTAGAAATGCGCTTACAAAGATTAACCGGATTAGAA
ATTGAAAAAATTAACAATGAAATTAAGGATATTAAAGTGGCAATAACCGAATTAGAAGAA
ATTATTAATAATAGTGAAAAACAAATTGACATTATGATTACGGAATTAACAGAAATTAAG
AAAAAATATGGTGATGAGCGTCGAAGTCAAATTATTAAAGAAGAGCTAACAGAAATTGAT
CCAGAAGAATTAATTCGCCGGGAAGACATTTTAATTATGTTAACACAAGATGGTTATGTT
AAACGAGTATCTGATGATACTTTTAAATTACAAAATCGTGGTGGGAAAGGGATTATTGGT
TTAGCAAATATTGAAGATAGTTTAAAAAAAATTATTAGTGCTAATAGTATTGACTCATTA
TTAATTTTTTCAAATTTTGGGAAAGTGTATAAATTACGAGCTTATAATATTGAAACATAT
TCACGGCATGCACGGGGATTACCAATTATTAATTTAATTGCAATTGATAAAACAGAAAGT
ATTCAAACAATTATTGCTGTAGATGAATCAATTTCAACAGAGGATAATTTATTTTTCGTA
ACAAAAAAAGGTCTTATTAAAAAAACAAAAATAAGTGAATTTAATCAAATTCGCCAAACA
GGGAAAGTAGCAATTGAATTAAAAGATAATGATGAATTAGTAAGTGTGATTATTACAAAA
GGATATGATGATGTTATTATTGGAAATAATATTGGAAAAGTAATTCGATTTAATGAAAAG
GAAATTAGACCAATGACGCGACAATCAGTTGGGGTTAAAGGAATTGCTAATGATGCTACT
GGAGAAACAATTGGGGTGAGTTCAGGGCGGTCTGCGAAATATGTTTTATCAGTAACTGAA
AATGGTTATGCAAAAAAAACACTAATTAAAGATTATCGTTTAACAGGACGAAATACTAAG
GGTGTTAAGTCAATGAACTTAACAGATAAAACAGGGTATTTAAAATTTGTCCAAACTGTA
GATGGTGATGAAGACATTTTAGTAGGAACAAAAAAAGGAAATATTATTCGTGTTTCGCTA
GAGCAAATTCCATTATTTGGACGAACAACATCAGGTGTTAAAATTATGCGATTAGAACAA
GATGATAAATTAGAAGTTATTGAAATTTTAAAAAAGAAAATTAACATTGAAGGAGAAAGA
TAA

>protein sequence
MKSENDGYDYDGKIRDIDIADEMKNGFLDYAMSVIVSRAIPDVRDGLKPVHRRIIYAMWD
LKMTYEKQHKKSARIVGEVIGKYHPHGDTAVYEAMVRMAQDFSYRYPLIDGHGNFGSMDG
DAPAAMRYTEAKMSKIAGELIKDIEKETTIFIDNYDGSEEEPTFLPGYFPNLLVNGASGI
AVGMATNIPPHNLNEVIDGVIAVTKNPEITTVELMKIIKGPDFPTGALITAGNGLIKAYE
TGNGAITIRSKIDIEENNNKRRIIVSEIPYQINKAKLLERIAELVRDKIINGISDLRDES
NMEGVRVVLELKRDAQENVILNKLYKLTPLQTNFSLNILALYKNQPRVMGLKQILKYFIE
HQIDIIIKRSQFELKKNRNRLIILEGLKIALDNIDEVIAIIKQSQTTQIASDALINKFNL
VGEQARAILEMRLQRLTGLEIEKINNEIKDIKVAITELEEIINNSEKQIDIMITELTEIK
KKYGDERRSQIIKEELTEIDPEELIRREDILIMLTQDGYVKRVSDDTFKLQNRGGKGIIG
LANIEDSLKKIISANSIDSLLIFSNFGKVYKLRAYNIETYSRHARGLPIINLIAIDKTES
IQTIIAVDESISTEDNLFFVTKKGLIKKTKISEFNQIRQTGKVAIELKDNDELVSVIITK
GYDDVIIGNNIGKVIRFNEKEIRPMTRQSVGVKGIANDATGETIGVSSGRSAKYVLSVTE
NGYAKKTLIKDYRLTGRNTKGVKSMNLTDKTGYLKFVQTVDGDEDILVGTKKGNIIRVSL
EQIPLFGRTTSGVKIMRLEQDDKLEVIEILKKKINIEGER