SPM_002545

Uniprot: A0A037UM27
Description: tRNA(Ile)-lysidine synthase
EC number: 6.3.4.19
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O. {ECO:0000256|HAMAP-Rule:MF_01161, ECO:0000256|SAAS:SAAS00054827}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. {ECO:0000256|HAMAP-Rule:MF_01161, ECO:0000256|SAAS:SAAS00557872}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 17 22 ATP. {ECO:0000256|HAMAP-Rule:MF_01161}.
Site:
Gene names (primary): tilS
Gene names (synonym):
Mass: 51,675
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; ligase activity, forming carbon-nitrogen bonds [GO:0016879]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0005524; GO:0005737; GO:0006400; GO:0016879
Chain:
Signal peptide:
Domain [CC]: DOMAIN: The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. {ECO:0000256|HAMAP-Rule:MF_01161}.
Sequence similarities: SIMILARITY: Belongs to the tRNA(Ile)-lysidine synthase family. {ECO:0000256|HAMAP-Rule:MF_01161, ECO:0000256|SAAS:SAAS00557866}.
Protein families: TRNA(Ile)-lysidine synthase family
Coiled coil:
Domain [FT]: DOMAIN 11 201 ATP_bind_3. {ECO:0000259|Pfam:PF01171}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92894
UniPathway:
CDD:
Gene3D: cd01992
HAMAP: 3.40.50.620
InterPro: MF_01161
PANTHER: IPR014729;IPR011063;IPR012094;IPR012795
PIRSF: PTHR11807:SF2
PRINTS:
PROSITE:
Pfam:
ProDom: PF01171
SMART:
SUPFAM:
TIGRFAMs:

479322-480627(+)

>nucleotide sequence
ATGGAAACTAAACTTTTAAAACAGAATGCAAAATATGTTATTGGTGTGTCTGGTGGTCCG
GATAGTATGTTTTTACTTGATAATATTTATAATTCAGAATTAGATATTAATAATTTTATT
GTGTGCTTGGTAAATTATCAGAAGCGAAAAGATAGTGACTATGATGAAGAAATTGTTGTT
GAATATTGTCAAAAACGAAATATTAAAATATGTGTAAAAAAAGTAACCGCAAGTGACTAT
GAACAATATCAGGTTAATTCCCATAATTTTCAAGCAGTTGCTCGTGAGTTACGATATGAT
TTTTTTTTACAAATTAGTGAAAAATATCATTGCCAGGAAGTTTTAATTGCCCATAATTTA
ACTGATCATATTGAAACATATATTTTGCAAAAGCAACGAAATGGAATTGTTGAATATTAT
GGTCTAAATAAAACTAGTTATTATTATTCTCAATTGTTGGACAAAAAACTAAAAATATTG
CGAATTATGTTAAAAATATCAAGAGAAGAAATTATTGAATATTTATTTCATCAGCAAATT
AATTATGCGATTGATTCAACTAATATTTTAGCAATTTATAATCGAAATATTATTCGTAAT
GAAATTCAGCATATTAATTTACAAGATATGTTATTAGAAATTGAACAAAGAAATAAAGAA
AATGAACAATTAAAATTAATTGCAAAAAATTATTTGATTGAAAATTTTGGACAAATTAAT
GTTACTAGTTTTAATGCTCTTAATAATATTCAGTTGCAAAAAATTATTATTTTTAATTAT
TTTAAAACTAATAAAGTTGTTCATTTAATTAGTAATAAAAAAAGAAAGTTTTTAGATGAA
ATAATTAAGGAATTAAGAAGTTTAAAACCAAATATTATTCTTAAAATTAATTCAAGATAT
TCTTTAATTAAATCATATGATAATGTTGACATTGTTAATAACGAGCTTTTAGAATTAACA
ACAATTCAAATTACACCAACAACTGTGTTACCAATAAGATGAAAAGAAAATATTATTAAT
TGGGGAACTAAAAATAAGTACGATTTTAAAATAACAGCAGCACAATGACCAATCATAATT
ACTAATGATTTATCTTTGTTAAGAAATGTAATGGTTAATAAGGTACGTTTAAATCGTTGA
TTTATTAAAAATAAAGTACCACTTTTAGCACGAAAAAGTTATTTTGTGTTAGATAAAAAT
AATAATCTTATTTATGGAAACAATTTAGTTGATTTAAAAATTAATAAATTTATTAATAAT
ACTTTAGCGGAAAAATACAATTTATTTTTTTTTATGATAAAATAG

>protein sequence
METKLLKQNAKYVIGVSGGPDSMFLLDNIYNSELDINNFIVCLVNYQKRKDSDYDEEIVV
EYCQKRNIKICVKKVTASDYEQYQVNSHNFQAVARELRYDFFLQISEKYHCQEVLIAHNL
TDHIETYILQKQRNGIVEYYGLNKTSYYYSQLLDKKLKILRIMLKISREEIIEYLFHQQI
NYAIDSTNILAIYNRNIIRNEIQHINLQDMLLEIEQRNKENEQLKLIAKNYLIENFGQIN
VTSFNALNNIQLQKIIIFNYFKTNKVVHLISNKKRKFLDEIIKELRSLKPNIILKINSRY
SLIKSYDNVDIVNNELLELTTIQITPTTVLPIRWKENIINWGTKNKYDFKITAAQWPIII
TNDLSLLRNVMVNKVRLNRWFIKNKVPLLARKSYFVLDKNNNLIYGNNLVDLKINKFINN
TLAEKYNLFFFMIK