SPM_002715

Uniprot: A0A037UQX4
Description: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoB
Gene names (synonym):
Mass: 146,455
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; ribonucleoside binding [GO:0032549]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351; GO:0032549
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta chain family. {ECO:0000256|HAMAP-Rule:MF_01321, ECO:0000256|RuleBase:RU363031}.
Protein families: RNA polymerase beta chain family
Coiled coil:
Domain [FT]: DOMAIN 169 534 RNA_pol_Rpb2_1. {ECO:0000259|Pfam:PF04563}.; DOMAIN 207 377 RNA_pol_Rpb2_2. {ECO:0000259|Pfam:PF04561}.; DOMAIN 441 489 RNA_pol_Rpb2_2. {ECO:0000259|Pfam:PF04561}.; DOMAIN 548 616 RNA_pol_Rpb2_3. {ECO:0000259|Pfam:PF04565}.; DOMAIN 626 691 RNA_pol_Rpb2_45. {ECO:0000259|Pfam:PF10385}.; DOMAIN 753 1136 RNA_pol_Rpb2_6. {ECO:0000259|Pfam:PF00562}.; DOMAIN 1138 1212 RNA_pol_Rpb2_7. {ECO:0000259|Pfam:PF04560}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92926
UniPathway:
CDD:
Gene3D: cd00653
HAMAP: 2.30.150.10;2.40.270.10;2.40.50.150;3.90.1110.10
InterPro: MF_01321
PANTHER: IPR019462;IPR015712;IPR007120;IPR010243;IPR007121;IPR007644;IPR007642;IPR007645;IPR007641;IPR014724
PIRSF: PTHR20856
PRINTS:
PROSITE:
Pfam: PS01166
ProDom: PF04563;PF04561;PF04565;PF10385;PF00562;PF04560
SMART:
SUPFAM:
TIGRFAMs:

519727-523636(+)

>nucleotide sequence
ATGGCATTAAAAGAAAAAGAATTTGGGCATTACGCTAAAAGAATTGATTACACAAAAGTA
AGTGGGAATTTAGATTTACCAAACTTAATTGAAATTCAAACTGAAACATATGATTGATTC
AAAAAAACAGGGATTAGCGAAGTGTTCGGGGAGGTATTTCCGATTGTTGGGCATGAAGGA
AATATCGTTTTAGAGATGTTAGATTGAGAATTTAGAGAGCCACGAAGAACAATTTCTCAA
GCAAAAGATGAGTCAAAAATTTTTGAAGCACCAATTTATTCAAATTTAAAATTAACAATT
AATTCAAAAGATGTAGAAGTTGAAAAAGCAATTGTTAAGGGAGAACCCGAATTAATTAAA
GCGTGAATTGAAGAACGTGTTGGGCATATGATTACAATTTTAAAAAAAACTACTGATGTT
ATTTATTATGATATTCATTCTGGCGATGAAATAGCAACATGTACAGTAACAATTAAAGAA
AGATTAGATGATAAACTAATTGTTGATATTACAATTGAAAAAGAAGGTGAAGTCTTTTTT
GGTGATTTTCCACTAATGACAGGAAAAGGAACTTTTATTGTTAATGGTTCAGAAAAAGTT
GTTGTTTCACAATTAGTTCGCTCACCCGGAGCATATTATAAAATTGATTTAAATCGTAAA
AATGGAGAAAATGTTTATTATGTTGATTTAATTCCGTCACGAGGAACATGGTTAGAATTT
GAATCAGATCATAAAAAAATTAAAGTTGGAAAAGAAGAAAAATTTGAGAATGTTTTTTAT
GTAAAAATAGATAAATCTAGAAAAGTATCGGTTGCTAATTTCTTAACAGCGTTAGGAATT
ATTAAAGAAGATGCCCTAGATATTTTTGGCGATAATAAATTAGTAAAAAGTACTTACGAA
TTAGATCCTTATACAGGTGATATTTTATATGACCAAAGTATTGCTGTTCAAGAAATTTAT
AAAAAAATTCGTTCAGGAGAAACGGCAACACCAGATGGTGCGACAAAATATTTATATGGA
TTATTATTTGATAAACGTAAATATGATTTAACAAAAGCAGGACGTTTTAAATTAATTCAA
AAATTATCAGTTGAAAACCGAATTTATAACAAAATATTAGCTGAAGACATTAAAGATGTA
AATGGTAAAGTTGTTTTTACCGAAGGAACTTTAATGGATAAAGAAGCTATTAACAAGTTA
AAAGGAATTTTAAAAGCAGGTGCTTGTTTACAAGAAGTTAAATTTAGTGATGAAATTATT
TGTTCTAATAAAATCCAAAAAATTAAAGTATATGTTGATAATGAAGTTCGTTCTCGTGTG
GCAAATATTATTGGAATTGATCCTAATGCAACTGATGAATATGTAACAGTTCCTGATGTC
TTGGCAACATTTTCATATTTATTAAATTTAACAGATGGAATTGGGGAAGTTGATGATATT
GACCATTTAGGAAATCGTCGTGTCCGAACAATTGGGGAATTATTACAAAATCAATTTCGA
ATTGGATTACTAAGAATTGAAAAAAATGTTAAAGAAAAAATGTCAACTTCAAATTTATTT
AAAATGAAACCTTCAAATATTATTAATAATAAACCATTATCAGCGATTATTGGGGAATTT
TTCAATTTATCACAGTTATCACAATTTATGGATCAAACAAATCCTTTAGCTGAATTAACA
AATAAACGTCGATTAACAGCCCTAGGGCCAGGAGGACTATCAAGAGAACGTGCTGGATTA
GAAGTCCGAGATGTTCACTACTCACATTATGGCCGTATTTGTCCAATTGAAACACCAGAA
GGACCAAATATTGGATTAATTAATAACTTAGCAACATATGCCAAAATTAATTCATATGGA
TTTATTGAAACACCATATCGTCGTGTTATTGGTTGCAAAGTTACCATGGAAAATGATTAT
TTAACAGCGGATGAAGAAAAAAATTATGTTGTTGCACAAGCTAATATTCGTTTAAGTGAT
AAGGGTGAAATTTTAGATGAACAAGTGGTTGCACGTTTCCAAGGAGAAAATATTATTGCC
GGACGAAATGATGTTGACTATGTTGATGTTTCACCAAAACAAATTGTTTCAATTGCAACA
AGTTGTATTCCATTTTTAGAAAATGATGATGCCAATCGTGCTTTGATGGGAGCCAATATG
CAACGACAAGCAATTCCTTTAATTGCGCCAAATTCACCATATGTAGGAACAGGAGTTGAA
TATGCTGCTGCTCGTGACTCGGGATTAGCAATTGTTTCACAATATGATGGAATTGTTGAT
TTTGTTGATGCAACAAGAATTGTTTTAAAAACAAAAGAAGGGTTAAAAAATTATAATTTA
GACACTTTTGTTCGTAGTAACCAAGGGACATCATTAACCCATGTGCCATTAGTGCGTCAA
GGACAAAAAGTTGAAAAAGGACAGGTTTTAGCTGATGGGCCATCAATTGATAAAGGGGAA
CTAGCCTTAGGACAAAATGTGGTAGTTGCTTTTACAACATGAAATGGTTACAACTATGAA
GATGCTATTATTGTTTCAGAACGCTTAGTATCAGAAGATGTTTTTACATCAATTCATATT
GAAGAATATACAATTGAACGTCGTCAAACAAAACAAGGACCAGAAGAAATTACGCGTGAG
ATTCCTAATATTTCTGAAAATGCACGCAAATTTTTAGATGATGATGGATTAGTTATTATT
GGGACAGAAGTTAAACCAGGTGATATTTTAGTTGGAAAAGTAACACCAAAAGGACAAACC
CAATTATCACCAGAAGATAAATTATTGCAAGCAATTTTTGGTGAGAAATCAAAAAATGTT
AAAGATAATTCATTACGAGTTCCAAATGGTGGTGAAGGAATTATTCAAGCAATTAAACGC
TTTCCACGAGAAAAATATGAATTATCAGCTGATGTTTTAGAAGTTATTAAGATTTATATT
GTTCAAAAACGAAAAATCCAAGAAGGAGATAAAATGGCTGGTCGTCATGGAAACAAAGGG
GTTATTTCGAAAATTTTACCATTGGAAGATATGCCACATATGGAAGATGGAACACCAGTT
GATATTATGTTAAATCCATTGGGGGTACCATCACGGATGAATATTGGACAGGTGTTAGAA
ATTCACTTAGGAATGGCAGCGAAAAAGTTGGGACAAAAAATTTCAACGCCTGTTTTTGAT
GGAATGACAAATGAAGAATTAATTGAAATTATGGATAAAGCAGGAATGAAAAACTTTGGG
AAAGAAGTTTTAATTGATGGTCGAACAGGTGAAAAATTTGATAATCCAGTTTCGGTAGGA
GTAATGTATATGTTGAAATTATCACACATGGTTGATGATAAATTACATGCGCGAAATGTC
GGATCATATTCATTAATTACGCAACAACCATTAGGAGGAAAAGCTCAAAATGGGGGACAG
CGTTTTGGAGAAATGGAAGTATGAGCATTAGAAGCATATGGTGCAGCCCATACATTGCGT
GAAATTTTAACAATTAAGTCTGATGATATTAAAGGAAGAACACGTGCATATGAATCAATT
GTTAAAGATAAAAAGATTCCAGAACCAGGGATTCCAGAATCATTTAATGTGCTAACTCGT
GAAATCCAAGGGTTAGGATTTAATATTCATATGATTGATGAAAAAGGAAATATTAAAAAT
ATTAAGAGTTATGATGAAGCAGATTATGTTGATGAAGATTTATTAACAGATTGTGATGAA
TTTGAAGATGAATTTGATATTGATAATTTTATTTTAAGTACTAATCGTGAACCACAAAAA
AAAGAACATTTAGAGGACTTTGTTAAAGTTGAAGATAGTTTTGATTTGGTAGATAACTTA
GATGATAATGAACTTGATGACATTGACAATGAGATTGATGAAATTAATGATCAAGATGAA
TTAGATTAA

>protein sequence
MALKEKEFGHYAKRIDYTKVSGNLDLPNLIEIQTETYDWFKKTGISEVFGEVFPIVGHEG
NIVLEMLDWEFREPRRTISQAKDESKIFEAPIYSNLKLTINSKDVEVEKAIVKGEPELIK
AWIEERVGHMITILKKTTDVIYYDIHSGDEIATCTVTIKERLDDKLIVDITIEKEGEVFF
GDFPLMTGKGTFIVNGSEKVVVSQLVRSPGAYYKIDLNRKNGENVYYVDLIPSRGTWLEF
ESDHKKIKVGKEEKFENVFYVKIDKSRKVSVANFLTALGIIKEDALDIFGDNKLVKSTYE
LDPYTGDILYDQSIAVQEIYKKIRSGETATPDGATKYLYGLLFDKRKYDLTKAGRFKLIQ
KLSVENRIYNKILAEDIKDVNGKVVFTEGTLMDKEAINKLKGILKAGACLQEVKFSDEII
CSNKIQKIKVYVDNEVRSRVANIIGIDPNATDEYVTVPDVLATFSYLLNLTDGIGEVDDI
DHLGNRRVRTIGELLQNQFRIGLLRIEKNVKEKMSTSNLFKMKPSNIINNKPLSAIIGEF
FNLSQLSQFMDQTNPLAELTNKRRLTALGPGGLSRERAGLEVRDVHYSHYGRICPIETPE
GPNIGLINNLATYAKINSYGFIETPYRRVIGCKVTMENDYLTADEEKNYVVAQANIRLSD
KGEILDEQVVARFQGENIIAGRNDVDYVDVSPKQIVSIATSCIPFLENDDANRALMGANM
QRQAIPLIAPNSPYVGTGVEYAAARDSGLAIVSQYDGIVDFVDATRIVLKTKEGLKNYNL
DTFVRSNQGTSLTHVPLVRQGQKVEKGQVLADGPSIDKGELALGQNVVVAFTTWNGYNYE
DAIIVSERLVSEDVFTSIHIEEYTIERRQTKQGPEEITREIPNISENARKFLDDDGLVII
GTEVKPGDILVGKVTPKGQTQLSPEDKLLQAIFGEKSKNVKDNSLRVPNGGEGIIQAIKR
FPREKYELSADVLEVIKIYIVQKRKIQEGDKMAGRHGNKGVISKILPLEDMPHMEDGTPV
DIMLNPLGVPSRMNIGQVLEIHLGMAAKKLGQKISTPVFDGMTNEELIEIMDKAGMKNFG
KEVLIDGRTGEKFDNPVSVGVMYMLKLSHMVDDKLHARNVGSYSLITQQPLGGKAQNGGQ
RFGEMEVWALEAYGAAHTLREILTIKSDDIKGRTRAYESIVKDKKIPEPGIPESFNVLTR
EIQGLGFNIHMIDEKGNIKNIKSYDEADYVDEDLLTDCDEFEDEFDIDNFILSTNREPQK
KEHLEDFVKVEDSFDLVDNLDDNELDDIDNEIDEINDQDELD