SPM_002720

Uniprot: A0A037UNH4
Description: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00054052}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279, ECO:0000256|SAAS:SAAS00054030}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoC
Gene names (synonym):
Mass: 140,130
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000256|HAMAP-Rule:MF_01322}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta' chain family. {ECO:0000256|HAMAP-Rule:MF_01322, ECO:0000256|RuleBase:RU004279}.
Protein families: RNA polymerase beta' chain family
Coiled coil: COILED 232 259 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 276 555 RPOLA_N. {ECO:0000259|SMART:SM00663}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92927
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_01322
PANTHER: IPR012754;IPR000722;IPR006592;IPR007080;IPR007066;IPR007083;IPR007081
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF04997;PF00623;PF04983;PF05000;PF04998
SMART:
SUPFAM: SM00663
TIGRFAMs:

523653-527403(+)

>nucleotide sequence
ATTGAAAATAATGAAAAAAATAATCGTATGATTAAAATCGGCTTAGCAAACCCCGATGAT
ATTCGCAGTTGGTCATTTGGGGAAGTAAAAAAACCAGAAACAATTAATTATAAAACATTA
AAACCAGAACGTGATGGATTATTTGATGAAAAGATTTTTGGCCCAACCAAGAATTTTGAG
TGTGCTTGTGGGAAGTACAAAAAATCAAAAAATAAGGGAAAAATTTGTGAACGTTGTGGT
GTAGAAATTACTGAAGCGATTGTTCGTCGTGAAAGAATGGGACATATTGAATTAGAAGAA
CCAGTGACACATATTTGAATGTTAAAAGCAGCACCAAGTCGGATTGCTTTAATTTTAGAT
ATGAAGACAAAAGAACTTGAAGAAGTTGTTTACTTTGTTTCATATATTGTGTTAGATGCT
GGTGATGCAAAATCATTAAAACAAAAAATGGTATTAGATTTAGGAAATGCTAAAACATCA
GCCCAAACACGTCAACGATTAACAAAAACATTATGTGAAATTTTGGATACACTAGAACCT
GACACAATTGCTTATGAAGTGGGAGAAACAATGATCGAATATTTAAAAAATACTTCGTTA
CCATTTTCAATGGATGAATGTGCCCAATTCATTAATCGTCATACTAATGCACGCTTTGGA
ATTGGCGCAGAAGCGGTGGAAGTATTATTAAAAAACTTAAACATTGATGATGAAATTGAA
AAAATTAAGCAAGATTTAAAAGATAAAAAAACACAATTGGATCAAAATAAATTAATGAAA
CGTTTAGAAGTTTTAGATTCGTTAAAAAAATCAGGTTCACGACCAGAATGAATGATCTTA
CGAGCAGTACCAGTAATTCCGCCTGATATTCGCCCAATTATTCAATTAGATGGTGGTCGT
TTTACAACATCAGAGATTAATGATTTATATCGTCGAATTATTATTCGAAATGAACGATTA
AAAAAAGTTAAAGCAATGGGGGCACCAAGTGTTATTGTTAATAATGAAAAAAGGATGCTA
CAAGAAGCCGTTGATGCTTTATTAGATAATGAACGCAAAGCGCGACCAGTAACTGGAAAA
GATAAACGCCCATTAAAATCATTAACAAGTATTTTAAAAGGGAAACAAGGTCGTTTCCGT
CAAAATTTATTAGGGAAACGAGTTGACTATTCAGGACGTTCAGTTATTGCAATTGGTCCT
GACTTAAAAATGTATCAATGTGGATTACCACGTGATATGGCCATTACATTATTTAAACCA
TTTGTTATTAGCAAATTAGTTAAAGATGGTTTAGCAGCAAATATTAAAGTTGCGGAAAAA
CTAATTTTAAATCAAGACGATAAAGTATGAGAAGTTTTAGAAGAAGTTATTAAAAGTCGG
CCAGTGTTATTAAACCGTGCACCAACTTTACACCGATTAGGAATTCAAGCATTTGAGCCA
AAATTAGTAAAGGGGAAAGCAATTAGATTACATCCATTAGTAACAACTGCTTTTAATGCT
GACTTTGATGGTGACCAAATGGCGGTTCATGTACCAATTACAGAAGAAGCAGTTGCTGAA
GCACGAAGTTTAATGTTAGGAAGTCGAAATATTTTAGGTCCAAAAGATGGTAAGCCAATT
GTTACACCAACCCAAGATATGGTGTTAGGGAATTACTATTTAACCTATGAAGAAAAAGGA
CAATTAGGAGAAGGAACAATCTTTAAAGATTTAAATGAAGCTGTTATTGCATATGAAACT
GGAGCTGTCGCTTTGCATGCTTTAGTTGCCATCCCTGTTGCTGGTTTTGTAAATAAAAAA
TTCAATTCAGAACAAATGAAAGATTATATTATTACAACACCAGGAAAAATTATTTTTAAT
CAAATTTTTAAAGAAGAGTTTCCATACTTAAATGAACCTAATATTGAAAATTTAACAGCA
TTACCAGCGCGCTCATTAATTAAAGACAATGTTAACTTGGTTGAATACTTAGCTACTTGA
AAAGTTAATCCACCGTTTAAGAAAAAAGATTTATCAAATATTATTGATCGCTACTTTAAA
CAATATGGAGCTAATAAGACGGCTGAAATGTTAGATAACATGAAAAACCTTGGTTTCAAA
TATTCAGGGAAATCGGGTGTAACAGTATCAGCAGGCGATGTTAAAGTATACGACAAAAAA
CACGAAGAATTTAAAGCGGCAGATCAAAAAGTAAAAGAAATTAATGATTACTTTAAAATG
GGAATGTTAACAAGTCGTGAAAAACAACACCGTATTATTAGCGTTTGATCAAAAGTTAAA
GATAACATTCAAACTGAACTAGAACATGTTTTACGTGAAGACCCAAAAAATCCAATCTTT
ATGATGGCGGATTCAGGTGCACGGGGAAATGTCTCAAACTTTACCCAATTAGTTGGGATG
CGGGGATTAATGAATAATCCAAAAGGAGATATTATTGAATTACCAATTAAGTCTTCTTTC
CGTGAAGGCTTAACAGTGTCAGAATTCTTTATTTCAACCCATGGGGCGCGAAAAGGAATG
GCGGATGTTGCTTTAAAAACAGCTGACTCGGGATATTTAACAAGACGATTAGTTGATGTT
TCGCAAGAAATTATTATTACAATGAAGGACTGTAATGCTCGTCGTGGTTTTGTTGTATCA
GATATTATTGAACAAAAACATGCTAATATTATTGTGCCATTATTTGACCGTTTAGTTGGT
CGCTATAATTTAAAAGATATTAAATTAAAAAATGGTGAAGTTATTCTTGCTAATACATTA
TTGAGCGAAGAAGATAGTAGAAAAATTGTTGATAATGATATTAAGGAAGTTATTATTCGT
TCTGTTTTAACTTGTGAAGCAGAAAAAGGTGTTTGTCAACGTTGTTATGGAAAAAACCTT
GCAACAGGAATGGAAGTTGAAATTGGTGAAGCAGTTGGTACAATCGCTGCGCAATCAATT
GGTGAACCAGGGACACAGTTAACAATGCGAACATTCCATACTGGTGGTGTGGCTGGGGGA
GCTGATATTACCCAAGGGTTACCACGGATTAAAGAATTATTAGATGTAACCACACCAAAA
GGATCAATTGCTGTTATTTCTGAAATTGATGGAAAAATTAGTGACATTCGTGATGAAGGT
GGAATTCATACAATTTATGTTAAATCAGATAGTGATGAACGAAAATATAAAACACAATAT
AATGCTGTTTTAAGAATCAAAATTGGTGATAAAGTTGTTCGTGGTCAAAAATTGACTGAA
GGTTCAATTAACATTAAAGAATTATTAGAAGTTGCCAAAATTGAAGATGTTCATAATTAT
ATTTTAAAAGAAGTGCAACGAGTTTACCGTTTACAAGGAATTGAAATTTCTGATAAATAT
ATTGAAATTATTATTAAACAAATGTTGAATAAAGTTAAAATTATTGATGCTGGTGATACT
GAATTATTACCAGGGGAAATTGTAACGGTTAAACGTTATCGCAATGAAACAATTAACGCT
GTTCGTTCAATGAAAAAACCGCCAACGGCAAAACATGTTATTTTTGGAATTAAAAAAGCG
CCATTAGAATCTGAATCATTCTTATCATCAGCGTCATTCCAAGATACAACACGAGTATTG
GTTAAAGCAATTATTAAAGGCAAAGTTGATTGTTTAGAAGGATTAAAAGAAAACATTATG
TTAGGACATTTAATTCCAGCTGGAACAGGGTTAAAAAATCCAAAAGATATTATTACGGCC
GGAATTGCAGCGAAAGCTGAAGAATATTAA

>protein sequence
IENNEKNNRMIKIGLANPDDIRSWSFGEVKKPETINYKTLKPERDGLFDEKIFGPTKNFE
CACGKYKKSKNKGKICERCGVEITEAIVRRERMGHIELEEPVTHIWMLKAAPSRIALILD
MKTKELEEVVYFVSYIVLDAGDAKSLKQKMVLDLGNAKTSAQTRQRLTKTLCEILDTLEP
DTIAYEVGETMIEYLKNTSLPFSMDECAQFINRHTNARFGIGAEAVEVLLKNLNIDDEIE
KIKQDLKDKKTQLDQNKLMKRLEVLDSLKKSGSRPEWMILRAVPVIPPDIRPIIQLDGGR
FTTSEINDLYRRIIIRNERLKKVKAMGAPSVIVNNEKRMLQEAVDALLDNERKARPVTGK
DKRPLKSLTSILKGKQGRFRQNLLGKRVDYSGRSVIAIGPDLKMYQCGLPRDMAITLFKP
FVISKLVKDGLAANIKVAEKLILNQDDKVWEVLEEVIKSRPVLLNRAPTLHRLGIQAFEP
KLVKGKAIRLHPLVTTAFNADFDGDQMAVHVPITEEAVAEARSLMLGSRNILGPKDGKPI
VTPTQDMVLGNYYLTYEEKGQLGEGTIFKDLNEAVIAYETGAVALHALVAIPVAGFVNKK
FNSEQMKDYIITTPGKIIFNQIFKEEFPYLNEPNIENLTALPARSLIKDNVNLVEYLATW
KVNPPFKKKDLSNIIDRYFKQYGANKTAEMLDNMKNLGFKYSGKSGVTVSAGDVKVYDKK
HEEFKAADQKVKEINDYFKMGMLTSREKQHRIISVWSKVKDNIQTELEHVLREDPKNPIF
MMADSGARGNVSNFTQLVGMRGLMNNPKGDIIELPIKSSFREGLTVSEFFISTHGARKGM
ADVALKTADSGYLTRRLVDVSQEIIITMKDCNARRGFVVSDIIEQKHANIIVPLFDRLVG
RYNLKDIKLKNGEVILANTLLSEEDSRKIVDNDIKEVIIRSVLTCEAEKGVCQRCYGKNL
ATGMEVEIGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGGADITQGLPRIKELLDVTTPK
GSIAVISEIDGKISDIRDEGGIHTIYVKSDSDERKYKTQYNAVLRIKIGDKVVRGQKLTE
GSINIKELLEVAKIEDVHNYILKEVQRVYRLQGIEISDKYIEIIIKQMLNKVKIIDAGDT
ELLPGEIVTVKRYRNETINAVRSMKKPPTAKHVIFGIKKAPLESESFLSSASFQDTTRVL
VKAIIKGKVDCLEGLKENIMLGHLIPAGTGLKNPKDIITAGIAAKAEEY