SPM_003340

Uniprot: A0A037UR35
Description: diguanylate cyclase
EC number: 1.3.-.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (S)-dihydroorotate + acceptor = orotate + reduced acceptor. {ECO:0000256|HAMAP-Rule:MF_00224}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the conversion of dihydroorotate to orotate. {ECO:0000256|HAMAP-Rule:MF_00224, ECO:0000256|SAAS:SAAS00570238}.
Pathway: PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway. {ECO:0000256|HAMAP-Rule:MF_00224, ECO:0000256|SAAS:SAAS00570212}.
Active site: ACT_SITE 130 130 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_00224}.
Binding site: BINDING 21 21 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 45 45 Substrate. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 99 99 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 127 127 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 127 127 Substrate. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 166 166 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 192 192 FMN; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00224}.; BINDING 218 218 FMN; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00224}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 45 46 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; NP_BIND 244 245 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.; NP_BIND 266 267 FMN. {ECO:0000256|HAMAP-Rule:MF_00224}.
Site:
Gene names (primary): pyrD
Gene names (synonym):
Mass: 33,046
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; dihydroorotate dehydrogenase activity [GO:0004152]; 'de novo' pyrimidine nucleobase biosynthetic process [GO:0006207]; 'de novo' UMP biosynthetic process [GO:0044205]
Gene ontology IDs: GO:0004152; GO:0005737; GO:0006207; GO:0044205
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities:
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 3 283 DHO_dh. {ECO:0000259|Pfam:PF01180}.
Motif:
Region: REGION 69 73 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00224}.; REGION 193 194 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00224}.
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI93013
UniPathway:
CDD:
Gene3D:
HAMAP: 3.20.20.70
InterPro: MF_00224
PANTHER: IPR013785;IPR005720;IPR024920;IPR012135;IPR001295
PIRSF:
PRINTS: PIRSF000164
PROSITE:
Pfam: PS00911;PS00912
ProDom: PF01180
SMART:
SUPFAM:
TIGRFAMs:

629060-629984(+)

>nucleotide sequence
ATTACTATGTTAAAAACAAAATTTTTAGGATATACCTTAAATTGCCCTTTAACAACAGCA
AGTGGAACTTTTGGCTATGGTGAATGTTACCAGGACTTCTTTGCTGTTAATGAATTAGGA
ATGTTAACAACAAAAGGAATTACATTAGAACCACGATTAGGCAATAGTTCACCGCGATTA
GCAGAAGTTACAGGAGGATTAATTAATTCTATTGGTTTAGAAAACCCAGGGTTAGATTAT
TTTAAAGCAAAGATTGTTCCAACCTTTACCGCATTTCAGATTCCAATTATTGTTAATTTA
AATGGAAAAACAATTGAAGAATACGAAATATTAGCAACTGCTATTAATGAAATTGCTGCA
ATTGCTTTTGTTGAACTAAATATTTCTTGTCCAAATGTTAAAGAAGGGGGTATTTTGTTT
GGAACCGATCCAACAGTAACACGACAAGTAGTTAAAAAAGTTCGTCGTGTTTTAACAAAA
AAGAAATTAATTGTGAAATTGTCCCCATCAGTTAGTGACATTAAGCAATTTGCGATTATT
TGTCAAGAAGAAGGAGCAGATGCAATTAGTTTAATTAATACGATTCCTGCAATGCAGATT
AATCTTAAAACTAAAAGACCAGTGCTAGGTAATATTATAGGAGGATTAAGTGGACCTTGC
ATTAAACCAATTGCGATTCGGATGGTTTATGAAGTTGCTAGTGTGGTTACGATTCCAGTT
ATTGGGATGGGGGGTGTTAGTTCTGCCAATGATGTTCTAGAATTTTTAATGGCGGGAGCG
AGTATTGTTGCGATTGGTACTAGTTTATTTACAAATCCAAACCTTGTTTTAGAAATTAAA
AGAGATTTAGTAACATATTGTGCAGAACAAAATTTACAGCATTTAAGTGATATTGTGGGA
ATCGCTCACCAGAAGGAGAAATAA

>protein sequence
ITMLKTKFLGYTLNCPLTTASGTFGYGECYQDFFAVNELGMLTTKGITLEPRLGNSSPRL
AEVTGGLINSIGLENPGLDYFKAKIVPTFTAFQIPIIVNLNGKTIEEYEILATAINEIAA
IAFVELNISCPNVKEGGILFGTDPTVTRQVVKKVRRVLTKKKLIVKLSPSVSDIKQFAII
CQEEGADAISLINTIPAMQINLKTKRPVLGNIIGGLSGPCIKPIAIRMVYEVASVVTIPV
IGMGGVSSANDVLEFLMAGASIVAIGTSLFTNPNLVLEIKRDLVTYCAEQNLQHLSDIVG
IAHQKEK