SPM_003350

Uniprot: A0A037UTT4
Description: orotate phosphoribosyltransferase
EC number: 2.4.2.10
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00015847}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01208};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00015698}.
Pathway: PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00015801}.
Active site:
Binding site: BINDING 95 95 5-phosphoribose 1-diphosphate; shared with dimeric partner. {ECO:0000256|HAMAP-Rule:MF_01208}.; BINDING 99 99 5-phosphoribose 1-diphosphate; shared with dimeric partner. {ECO:0000256|HAMAP-Rule:MF_01208}.; BINDING 101 101 5-phosphoribose 1-diphosphate; shared with dimeric partner. {ECO:0000256|HAMAP-Rule:MF_01208}.; BINDING 125 125 Orotate. {ECO:0000256|HAMAP-Rule:MF_01208}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): pyrE
Gene names (synonym):
Mass: 22,359
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00216311}.
Gene ontology (GO): magnesium ion binding [GO:0000287]; orotate phosphoribosyltransferase activity [GO:0004588]; 'de novo' UMP biosynthetic process [GO:0044205]
Gene ontology IDs: GO:0000287; GO:0004588; GO:0044205
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00541116}.
Protein families: Purine/pyrimidine phosphoribosyltransferase family, PyrE subfamily
Coiled coil:
Domain [FT]: DOMAIN 48 154 Pribosyltran. {ECO:0000259|Pfam:PF00156}.
Motif:
Region: REGION 121 129 5-phosphoribose 1-diphosphate binding. {ECO:0000256|HAMAP-Rule:MF_01208}.
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI93015
UniPathway:
CDD:
Gene3D: cd06223
HAMAP: 3.40.50.2020
InterPro: MF_01208
PANTHER: IPR023031;IPR004467;IPR000836;IPR029057
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF00156
SMART:
SUPFAM:
TIGRFAMs: SSF53271

630685-631291(+)

>nucleotide sequence
ATGAAAAACATTATTACGGAATTAATTAAAATTAAAGCAATTAGTATTAATATTACAGAA
TTATATACTTGAGCATCAGGGATTAAAGCACCAATTTATATTGATAATCGATTAACGATG
GGTTATCCTCATTTACGGTGAGCAATTGCTGAAAGTTTTGCTGAACTTATTAGGACTAAT
TTAGGACAAGATAGAATTGATAATATTTTTGGAACAGCCACAGCAGGAATCCCCCATGCT
GCGCTATTGGGACATTTATTACAATTACCATTTGGTTATGTTCGCAGTAGTAAAAAAGAC
CATGGGAAACAAAATCAAATTGAAGGAGTCTATGAAAAGGGACAAAAGGTTGTTGTCATT
GAAGACTTAATTTCTACAGGAGGGTCGGTTATTGAAGTTGTTAAAACTTTACAAGCGGCC
GGAATGGAAGTTATTGCGGTATTAGCAATTTTTAGTTATCAATTAAAAAAGGCATGCGAT
GCATTTAATACGTTAGAAATTCCTTTCTATTCTTTAACTAATTTTAATAGTTTAGTAACA
ACTGAAGGTTTATTATCAAAAGCAGAACAACAAATTTTAAAAGAGTTTCATCACCAATTA
GAGTAG

>protein sequence
MKNIITELIKIKAISINITELYTWASGIKAPIYIDNRLTMGYPHLRWAIAESFAELIRTN
LGQDRIDNIFGTATAGIPHAALLGHLLQLPFGYVRSSKKDHGKQNQIEGVYEKGQKVVVI
EDLISTGGSVIEVVKTLQAAGMEVIAVLAIFSYQLKKACDAFNTLEIPFYSLTNFNSLVT
TEGLLSKAEQQILKEFHHQLE