SPM_003515

Uniprot: A0A037UTX5
Description: tRNA pseudouridine synthase B
EC number: 5.4.99.25
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55). {ECO:0000256|HAMAP-Rule:MF_01080}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_01080}.
Pathway:
Active site: ACT_SITE 40 40 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_01080}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): truB
Gene names (synonym):
Mass: 34,812
Subunit structure [CC]:
Gene ontology (GO): pseudouridine synthase activity [GO:0009982]; RNA binding [GO:0003723]; tRNA pseudouridine synthesis [GO:0031119]
Gene ontology IDs: GO:0003723; GO:0009982; GO:0031119
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_01080}.
Protein families: Pseudouridine synthase TruB family, Type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 25 176 TruB_N. {ECO:0000259|Pfam:PF01509}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI93045
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_01080
PANTHER: IPR020103;IPR002501;IPR014780
PIRSF: PTHR13767
PRINTS:
PROSITE:
Pfam:
ProDom: PF01509
SMART:
SUPFAM:
TIGRFAMs: SSF55120

662034-662949(+)

>nucleotide sequence
ATTATGCATGATGGTATTTTTTTAATCAATAAACCAACAGGAAAAACAAGTAATCAAGTT
ATTCAAGAAATTAAACAAAAATTAGAGATTAATAAAATTGGTCACGCAGGAACATTAGAC
CCATTAGCAACAGGATTGTTAGTTGTTTTAATAAATAATGCTACTAAAATGAGTGAATAT
TTGTTAACAGCAGATAAAGCGTATGATGTTGAAATGCGCTTATTTATTGAAACAGATACT
GGTGATATTACTGGAAATGTTCTTAATGAAACGGCTCCATTTAAGATTCAGAAGAAGGTA
TTAAAAAAAATTTTTAATGAATTTAATGGATTTATGTATGAACAATATCCACCCAAATAT
TCTGCTATTAAAGTTAATGGTAAAAAACTTTACGAATATGCTCGCCAAGATAAAGAAGTA
AAAATTAAACCAAGAACTGTTACAATTAAGGCGATTAAATTAAAAAAATATGATGCTCAT
AATCATACAATTAAATTTAGCGTGTTATGTACTAAGGGAACATATATTAGAAGTTTAGTG
ACTGATATTGCGGCAAAATTAAATACTATTGCTACTGTTACAGCTTTATGCCGCACGCAA
TCAGGAAATTTTTTGTTATCTAAAGCAATTAGCCCTGAAACAGTAAATTTTAATGAGTTA
ATATCGATGTATGATGCTTTATTTACTAATAACCAACAATTATTACTTTATCATCATGAT
GAAGAAATTCGACAAGGAAAACCGATTGTTATTATTAAACATACTGACCCAATTATTTTT
ATTATTGATAAAAATAAAAATGTTTTAGCAATTTATAAACATATTGGAAAACACGTATAT
GCTTGTCAACGTGGATTATGATCACATATTGAGCCTAAAAAACAAGATGATAGAGAGAAG
GACGATCATTATTAA

>protein sequence
IMHDGIFLINKPTGKTSNQVIQEIKQKLEINKIGHAGTLDPLATGLLVVLINNATKMSEY
LLTADKAYDVEMRLFIETDTGDITGNVLNETAPFKIQKKVLKKIFNEFNGFMYEQYPPKY
SAIKVNGKKLYEYARQDKEVKIKPRTVTIKAIKLKKYDAHNHTIKFSVLCTKGTYIRSLV
TDIAAKLNTIATVTALCRTQSGNFLLSKAISPETVNFNELISMYDALFTNNQQLLLYHHD
EEIRQGKPIVIIKHTDPIIFIIDKNKNVLAIYKHIGKHVYACQRGLWSHIEPKKQDDREK
DDHY