SPM_003570

Uniprot: A0A037URD8
Description: tRNA delta(2)-isopentenylpyrophosphate transferase
EC number: 2.5.1.75
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA. {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003783}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00185};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003784}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 11 18 ATP. {ECO:0000256|HAMAP-Rule:MF_00185}.
Site: SITE 102 102 Interaction with substrate tRNA. {ECO:0000256|HAMAP-Rule:MF_00185}.; SITE 120 120 Interaction with substrate tRNA. {ECO:0000256|HAMAP-Rule:MF_00185}.
Gene names (primary): miaA
Gene names (synonym):
Mass: 35,822
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00185}.
Gene ontology (GO): ATP binding [GO:0005524]; tRNA dimethylallyltransferase activity [GO:0052381]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0005524; GO:0008033; GO:0052381
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IPP transferase family. {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003785}.
Protein families: IPP transferase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 13 18 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00185}.
EMBL: AGBZ02000001
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI93056
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_00185
PANTHER: IPR018022;IPR027417
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01715
SMART:
SUPFAM:
TIGRFAMs: SSF52540

674077-675013(+)

>nucleotide sequence
ATGGTGGCGAAAAAAGTTATTTTAATTGTTGGACCAACAGGAAGTGGTAAAACTGCTTTA
TCAATTAAAATCGCTCAGCGTTTTAATGGCGAATGTATTAATGCTGATGCAACCCAAATT
TTTAATGGGTTAAATCTTGCAACAAATAAAGTAACACCATCTGAACAAGCTGGAATTCCA
CACCATTTATTATCAACAATTGATTTAAATGATAATTATTCCATTAAGATGTACCAACAG
CAGGGACGAAAAATATTAGCCCAATTATGAAGTAAAAATAAAATTCCAATTGTTGTTGGT
GGTAGTGGGTTATATATTAATGCACTATTAAAGAATTACCAATTTTCTGATCAGGGTCGT
GATTTATTAAAAACAAAGGAATATGAACATGTTTCAAATTATGCTTTATGAGAAAAATTA
GCAACAATTGATCCACAAGAGAGTACTAAAATTCATCCTAATAACCGAAAACGAGTTTGG
CGGGCATTAGAATATTATTTACAAACAGGAACATTAAAATCAAGTAATGATCATCACCGA
AATGAATGATATATTGAACCATATATTATTGGGTTATTACCAGATAAAGCAGAACTGCAT
CAATATTTATTTGATCGGGTGTTACGGTTAACAGAGCAAGGTTTATTTGCTGAAGTTGAA
GCAGCATATAAATATTGTAATTATGATTTAACAAAGCAAAGTATGAAAATTATTGGTTGT
TCAGAAATTATTCGTTATTTAAAAGGTGAGCTTTCATATGAAGCAACATTAGCAGCAATG
GTGCAAGCTAATAAAGTTTATGCTAAAAAACAAATCACATGGTTTAAACACCAATTAACA
AATGTGCATTGATATTCATTTTCCTATTCACAGTTTGATAATCTTTGCCAGAAAATTATT
GTTGAATTAAAAAATAGTAACTATTTAAAATTATAA

>protein sequence
MVAKKVILIVGPTGSGKTALSIKIAQRFNGECINADATQIFNGLNLATNKVTPSEQAGIP
HHLLSTIDLNDNYSIKMYQQQGRKILAQLWSKNKIPIVVGGSGLYINALLKNYQFSDQGR
DLLKTKEYEHVSNYALWEKLATIDPQESTKIHPNNRKRVWRALEYYLQTGTLKSSNDHHR
NEWYIEPYIIGLLPDKAELHQYLFDRVLRLTEQGLFAEVEAAYKYCNYDLTKQSMKIIGC
SEIIRYLKGELSYEATLAAMVQANKVYAKKQITWFKHQLTNVHWYSFSYSQFDNLCQKII
VELKNSNYLKL