SPM_003580

Uniprot: A0A037UR91
Description: NAD synthetase
EC number: 6.3.1.5
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+). {ECO:0000256|HAMAP-Rule:MF_00193, ECO:0000256|RuleBase:RU003812, ECO:0000256|SAAS:SAAS00016647}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. {ECO:0000256|HAMAP-Rule:MF_00193, ECO:0000256|SAAS:SAAS00016631}.
Active site: ACT_SITE 32 32 {ECO:0000256|HAMAP-Rule:MF_00193}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 30 37 ATP. {ECO:0000256|HAMAP-Rule:MF_00193}.
Site:
Gene names (primary): nadE
Gene names (synonym):
Mass: 27,661
Subunit structure [CC]:
Gene ontology (GO): ATP binding [GO:0005524]; NAD+ synthase (glutamine-hydrolyzing) activity [GO:0003952]; NAD+ synthase activity [GO:0008795]; NAD biosynthetic process [GO:0009435]
Gene ontology IDs: GO:0003952; GO:0005524; GO:0008795; GO:0009435
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the NAD synthetase family. {ECO:0000256|HAMAP-Rule:MF_00193, ECO:0000256|RuleBase:RU003811, ECO:0000256|SAAS:SAAS00541228}.
Protein families: NAD synthetase family
Coiled coil:
Domain [FT]: DOMAIN 8 233 NAD_synthase. {ECO:0000259|Pfam:PF02540}.
Motif:
Region:
EMBL: AGBZ02000001
ProteinModelPortal: A0A037UR91
MEROPS:
EnsemblBacteria KO: KAI93058
UniPathway:
CDD:
Gene3D: cd00553
HAMAP: 3.40.50.620
InterPro: MF_00193
PANTHER: IPR022310;IPR003694;IPR022926;IPR014729
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF02540
SMART:
SUPFAM:
TIGRFAMs:

676964-677714(+)

>nucleotide sequence
ATGAAAGAATTAAACCAATATTTAAGTTATTTAGTAAAATGAATTAAATCAGAAGTGACA
AAAGCCCACTGCCAAGGGGTTATTGTCGGTTTATCAGGTGGAATTGATTCGGCTGTTGTT
AGTTTATTAGCAAAACAAGCTTTTCCTGAGCAACATTTAACAGTTATAATGCCTTGTCAT
TCTGACTATTTTGATCAAGAATGTGCACAGTTGTTAGTTAATAATCATCAATTAAAAAAT
CATCTTGTTGATTTAACAGGAACATATGATAATTTAATTGCAACATTAGCTTTGCCACCT
CATCAATTAGCATTCGCTAATATAAAGCCACGGTTACGAATGACAACCTTATATGCTTTA
GCACAAAGTCACAATTATATGGTTTTAGGAACGGATAATGCTGATGAATGACATGTCGGA
TATTTTACTAAATATGGTGATGGTGCTGCTGATTTAGTCCCTATTATTCATTTACTAAAA
TCGGAAGTTCAGCAAGCAGCACAATTATTAGGTGTGCCATCAGCAATTATTTCACGTCCA
CCAACAGCGGGATTATGAGCTAGTCAAACTGATGAAAAAGAATTAGGGTTTACTTATCAA
CAATTAGATTATTATTTACAAGGAAAATCAGTTCCAACAGCGATTGCACAACGAATTAAA
CAATTAAATCAAAGTTCTGAACATAAACGACATTTACCTAAAGCACCACCAAATATTTTT
AGTTCGTTAATTTCTGTTAGTAAAGATTAA

>protein sequence
MKELNQYLSYLVKWIKSEVTKAHCQGVIVGLSGGIDSAVVSLLAKQAFPEQHLTVIMPCH
SDYFDQECAQLLVNNHQLKNHLVDLTGTYDNLIATLALPPHQLAFANIKPRLRMTTLYAL
AQSHNYMVLGTDNADEWHVGYFTKYGDGAADLVPIIHLLKSEVQQAAQLLGVPSAIISRP
PTAGLWASQTDEKELGFTYQQLDYYLQGKSVPTAIAQRIKQLNQSSEHKRHLPKAPPNIF
SSLISVSKD