SPM_004100

Uniprot: A0A037UM09
Description: DNA topoisomerase IV subunit A
EC number: 5.99.1.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000256|HAMAP-Rule:MF_00937, ECO:0000256|SAAS:SAAS00075911}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. {ECO:0000256|HAMAP-Rule:MF_00937}.
Pathway:
Active site: ACT_SITE 123 123 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000256|HAMAP-Rule:MF_00937}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site: SITE 43 43 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 79 79 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 81 81 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 92 92 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 98 98 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 122 122 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00937}.
Gene names (primary): parC
Gene names (synonym):
Mass: 130,097
Subunit structure [CC]: SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000256|HAMAP-Rule:MF_00937}.
Gene ontology (GO): chromosome [GO:0005694]; extrinsic component of plasma membrane [GO:0019897]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (ATP-hydrolyzing) activity [GO:0003918]; chromosome segregation [GO:0007059]; DNA topological change [GO:0006265]
Gene ontology IDs: GO:0003677; GO:0003918; GO:0005524; GO:0005694; GO:0006265; GO:0007059; GO:0019897
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily. {ECO:0000256|HAMAP-Rule:MF_00937}.
Protein families: Type II topoisomerase GyrA/ParC subunit family, ParC type 2 subfamily
Coiled coil: COILED 922 945 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 12 460 TOP4c. {ECO:0000259|SMART:SM00434}.
Motif:
Region:
EMBL: AGBZ02000002
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92482
UniPathway:
CDD:
Gene3D:
HAMAP: 3.30.1360.40;3.90.199.10
InterPro: MF_00937
PANTHER: IPR024946;IPR006691;IPR013760;IPR002205;IPR013758;IPR005741
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF03989;PF00521
SMART:
SUPFAM: SM00434
TIGRFAMs: SSF56719

35883-39318(-)

>nucleotide sequence
TTATTTTTTACTTTTATCTAAAATATCATGGATGGTTAATTGTAACTCTTTTGTTTCATC
AATTGCTAAATCTTTTTTAAATTTAGCTTTTGTTACACTCTTATTTTTAATTTCTTTAAA
ATCAACTCTTTGCATTGTTTGTTCATGATTATTTGAAGTAACATTTCTTAACCCTACCTT
AATTGTTCCGGCTGGAGCTTGTTCAATTGCTTTTAACCATTCTGTTCGTGATTTTACTTT
TATCGTAGACTTATCTTTTGGGTTATCTTTTTCACTATTATTAAACTCAAAATCAATTTT
TTGTTGTCTATTTTTAAGAAGAGTCTCCACTGTCGTAACCACTTTGCCAAAAGTTGGTTT
TTCTTCAGGAAGAGAAGGAACAAGCAGTTTTCTATTTTTTTGGTGATTATTTTGTTGTGC
TTGTGCTTTTACTAATGCTACTTTAGCTTGTTCACGTTGTTTCTTTTCAATTAGCATATC
TACTGTTGTTTTTACTTTTCCAAATGTTTTACGAACAAACAACGGTGATGGTTCTTGTTC
AGATGGCGCTGCAATTATGCTAGTTTCCACATTTGTTACTGGTTGTTCCGATTGTTGTTT
ACTTTGTTGTTCTTTTCGAAATTTCTTTTTTGTTTCTTTTATATTTTTATTTTTCTGGCG
CATTTTTTCTCCGCGTGGTTTAACCACAATGTTTTTGTTAATACTTTTCTTACTATTAGG
ATGATGGTCCAGCGTTGTTATTGGCTCTGCTGTTGCAATCACCTGCTCGACTTGATAAAT
TTCTTCTGGTGGAATCATTAATGCTAATTTAACCTTGAGATCACTTTCTTCATCGTTAGT
ATTATCTGCTGTGATTGTTTCTTGATTATAACTTTTTTCACTTGATTCGGTCTTATTCAG
AACAGAATTATTTGTTGCCTGTGTTACTTCAATTAAGGGTTTAATATTATTGGCATTATC
ATGTTCATTATGATGAAACGAATGCTTTTTTCCAGCAGTACTACTACTTTCTAATCCTTT
TTTTGATGGGATTACTTTTAAATCATAATAATGTGGAATAACAATATCAACAATATTTTC
AAAACCTAAGGTATGATTGGCAGTAACTAATTTATGAACAGCCATTTTATCAGTATTAAA
TAACTCAATTTCATCTTTTTGATTTAACATATGCAAGATATCTTTCCCTTTTGTTAAAAA
CGTTCAGCGAACAAGTTCATTAATATCTTTATTTAATTTATATAATCGTGTTCCTTTTAC
TGGTCGCGTTGATGTCGGGATTTCTGTTAAAGAAATTTTTTTCCCAGTATGAAGATTAGT
AATTAATACTAAATTATCATCTTCACAATAACCCGCGTTAATAACAACATCATCACGTAG
ATTAATTGATTTAACCCCTGCTGTTCGTAACCCTAATGTTGGAATTTGTTTTTTACGATA
TTTAACAATAAAACCATTTGCTGTTGTTATGACCACATATGGATGATGACCAGCTAATTG
ACTAGCAACAACTTCATCATCATTTTTTAAATTAATAGCTTTAAGCGCTTTACTAATCCG
TGTCGCTTCAAAATCTTTAACGGCTGTTTTTTTAATCATTCCATTTTTAGTTGCTAACAT
AATGTATTGTTCTTTTTTATTAAAATCATTTAATAAGAAAGCACTAATAATTTTTTCTTC
TCCTGGTAACTCAGAAATAGTATTAACATGCTCACCAATATCACGTAGTCGAACTGTTTT
AATTTTATAAACTGGAATAATAACATAATTCCCTTTTGAAGTAATTAACAAAATTTTATC
GGAAGTATTAGCTTGAAAATCAGCAATTCATAAATCCCCTGGTTTTCGTTTAAATTCTTC
GGTTGCTAATCGCGCTAATTGATTATTTTCTAAGGCTTTTAAATATCCATCTCGTGAAAT
TCAAATGTTCAAATCTTTTTCAATAATTGTTTCTGTTTTTTGAATTTCAATTGCTTCAAT
TTCATTTTCAATCACACTACGACGACGAGAAGCAAATTTTTTCTTAATTAATTCTAATTG
ATGAATAATAACACTATTTAATTTATTAATATCACGTAAAATTGCTTTTAATTCAGCAAT
TGTTGCAATTAATTCTTTTTGTTCTGCTTTTAATTGCAAAATATCCGTTGAAGTTAATCG
ATATAATCGTAATTGTACTATTGCTTCTGCTTGAATTTGGGTAAAACCAAATTTTTTTAC
TAAATTATGAATTGCATCACTACGATCTTTTGAATGGCGAATGACAGCAATCACTTCATC
TAAAATACTAATTGTTTTGATTAATCCAGCAACAATTTCTGAGCGTTTTTGTGCTCGTGC
TAATTCAAAGCGAGAACGATTAGTAATAACTTCTTGTTGATGTTCAACATAATGGGTTAA
AATTTCTTTTAGCCCTAATTGTCGTGGTTGTTTGTTTGCAATAGCAACCATATTAAAATT
ATATGAAATTTGTAAATTAGTATTTTTTAATAAAAATTTCCGTACTGTTTCAACATTAGC
TTGTTTACCTAATTCAATAACAATTCGTAACCCTTCGCGGTCTGTTTCATCACGAACTTC
TTTAATATTTAATCCTGGTTCATTATATTTAATATCATCAATCTTTTTAACTAAGTCTTG
TTTAACAACTTCATAGGGAATCTCAGTAATTACTAAAGCATTGTTTTCAAATTCAATTTT
GCTACGAACAATAATTTTTCCTTTTCCTGTTTGATATGCCTCTAAGATTCCACTTTTTCC
CTGAATAATGCCTCCGGTTGGAAAATCAGGTCCTTTAACATATTTTACTAAATCTTTAAC
CGTACTGTCTGGTTTTTCAATAAAATGAATTGTTGCATTAATAATTTCATGTAAATTATG
TGGTGGAATATTTGTGGCATATCCAGCAGCAATCCCGGTTGAACCATTAACTAATAAATT
TGGAAATAAGGCTGGTAATACAACTGGTTCTTGTTCTGAATCATCAAAATTAGGAGCAAA
CGCAACTGTTTTTTTATCAAGATCTTGTAATAAAAATGAGGAAATTTCACTTAAACGAGT
TTCAGTATAACGCATAGCTGCCGCTGGGTCACCATCAATTGAACCATTGTTTCCATGCAT
ATCAATTAATGGATAACGAACTTTTCAATTTTGAGATAACCTTACCATTGCATCATAAAC
CGATGTATCACCATGAGGATGATATTTTCCAATTACTTCTCCAACAATCCGTGCCGATTT
TTTATAACTCGTATTAAAAGTTAATCGTAATTCATTCATTGCAAATAAAATACGGCGTTG
TACTGGTTTTAAACCATCACGAACATCAGGTAAGGCTCGATCTTGAATAATATATTTGGC
ATAACGTCCAAAGCGTTCTGACATAATATCATTTAAACTATAAACTAATTCATTTTCTTT
TTCGTTATTACTCAT

>protein sequence
MSNNEKENELVYSLNDIMSERFGRYAKYIIQDRALPDVRDGLKPVQRRILFAMNELRLTF
NTSYKKSARIVGEVIGKYHPHGDTSVYDAMVRLSQNWKVRYPLIDMHGNNGSIDGDPAAA
MRYTETRLSEISSFLLQDLDKKTVAFAPNFDDSEQEPVVLPALFPNLLVNGSTGIAAGYA
TNIPPHNLHEIINATIHFIEKPDSTVKDLVKYVKGPDFPTGGIIQGKSGILEAYQTGKGK
IIVRSKIEFENNALVITEIPYEVVKQDLVKKIDDIKYNEPGLNIKEVRDETDREGLRIVI
ELGKQANVETVRKFLLKNTNLQISYNFNMVAIANKQPRQLGLKEILTHYVEHQQEVITNR
SRFELARAQKRSEIVAGLIKTISILDEVIAVIRHSKDRSDAIHNLVKKFGFTQIQAEAIV
QLRLYRLTSTDILQLKAEQKELIATIAELKAILRDINKLNSVIIHQLELIKKKFASRRRS
VIENEIEAIEIQKTETIIEKDLNIWISRDGYLKALENNQLARLATEEFKRKPGDLWIADF
QANTSDKILLITSKGNYVIIPVYKIKTVRLRDIGEHVNTISELPGEEKIISAFLLNDFNK
KEQYIMLATKNGMIKKTAVKDFEATRISKALKAINLKNDDEVVASQLAGHHPYVVITTAN
GFIVKYRKKQIPTLGLRTAGVKSINLRDDVVINAGYCEDDNLVLITNLHTGKKISLTEIP
TSTRPVKGTRLYKLNKDINELVRWTFLTKGKDILHMLNQKDEIELFNTDKMAVHKLVTAN
HTLGFENIVDIVIPHYYDLKVIPSKKGLESSSTAGKKHSFHHNEHDNANNIKPLIEVTQA
TNNSVLNKTESSEKSYNQETITADNTNDEESDLKVKLALMIPPEEIYQVEQVIATAEPIT
TLDHHPNSKKSINKNIVVKPRGEKMRQKNKNIKETKKKFRKEQQSKQQSEQPVTNVETSI
IAAPSEQEPSPLFVRKTFGKVKTTVDMLIEKKQREQAKVALVKAQAQQNNHQKNRKLLVP
SLPEEKPTFGKVVTTVETLLKNRQQKIDFEFNNSEKDNPKDKSTIKVKSRTEWLKAIEQA
PAGTIKVGLRNVTSNNHEQTMQRVDFKEIKNKSVTKAKFKKDLAIDETKELQLTIHDILD
KSKK