SPM_004130

Uniprot: A0A037UM14
Description: MTA/SAH nucleosidase
EC number: 3.2.2.9
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-homocysteine + H(2)O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. {ECO:0000256|SAAS:SAAS00064799}.; CATALYTIC ACTIVITY: S-methyl-5'-thioadenosine + H(2)O = S-methyl-5-thio-D-ribose + adenine. {ECO:0000256|SAAS:SAAS00064796}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. {ECO:0000256|SAAS:SAAS00064815}.
Pathway: PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. {ECO:0000256|SAAS:SAAS00064800}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary):
Gene names (synonym):
Mass: 25,267
Subunit structure [CC]:
Gene ontology (GO): adenosylhomocysteine nucleosidase activity [GO:0008782]; methylthioadenosine nucleosidase activity [GO:0008930]; L-methionine biosynthetic process from methylthioadenosine [GO:0019509]; nucleoside catabolic process [GO:0009164]
Gene ontology IDs: GO:0008782; GO:0008930; GO:0009164; GO:0019509
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. {ECO:0000256|SAAS:SAAS00561432}.
Protein families: PNP/UDP phosphorylase family, MtnN subfamily
Coiled coil:
Domain [FT]: DOMAIN 22 217 PNP_UDP_1. {ECO:0000259|Pfam:PF01048}.
Motif:
Region:
EMBL: AGBZ02000002
ProteinModelPortal: A0A037UM14
MEROPS:
EnsemblBacteria KO: KAI92487
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.1580
InterPro:
PANTHER: IPR010049;IPR000845
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01048
SMART:
SUPFAM:
TIGRFAMs: SSF53167

45811-46492(-)

>nucleotide sequence
TTATTCTTTATTATTAAATAATTTTGTCAAAATTAAATCAGAAATTAAAATTCCTGCTTG
TGATAAATTTTGTTTAAATTGTGTTGTTGAACTTTTTTTAGTTGCTTGTAAATAATCTGT
AATAATTTTAATACTAACAATTGGAATTTGATACTTTAATGAAACTTGAAAAAAAGCACA
ACCTTCCATATCCACCACATCAATTTTATTCTTAAATTTAGTATTAATATGATTAAAATA
TTCTAGTTTATCAATAAAAATATCACTGCTCCCTAAAACTGCTGGTTGAATGTTAGGTAT
TTTTGATGTAATTGTAGTTACTAATTTTTTATCACTATAATAACTAGGTAATTCACCTGG
AATTTGCCCCATTGTATAACCAAATTCTTGTGCATCAGCAAAAGTATAGTAAGCTTCAGT
AATCACTAAAACTGATAATGGTTTAAAAGTTGCCTTTAAAGTCCCAACTGTGCCAATATT
ATAAACTGTTTTAATTGCATAATCTTTAAGTAAGATTGTTAAAGTCATTGCAGCATTTAC
TAAACCAATCTTGCTAATTGCAAATAATCAATTACCTTTTTGATATAATTCAATCTGTGA
GTATTTACCAATTGCTGTTGGTTTTAAAGCATTTAAAGTTGTTATTAACTCTTCTTTCAT
TGCACTAATAATTAAATTCAT

>protein sequence
MNLIISAMKEELITTLNALKPTAIGKYSQIELYQKGNWLFAISKIGLVNAAMTLTILLKD
YAIKTVYNIGTVGTLKATFKPLSVLVITEAYYTFADAQEFGYTMGQIPGELPSYYSDKKL
VTTITSKIPNIQPAVLGSSDIFIDKLEYFNHINTKFKNKIDVVDMEGCAFFQVSLKYQIP
IVSIKIITDYLQATKKSSTTQFKQNLSQAGILISDLILTKLFNNKE