SPM_004345

Uniprot: A0A037UKF0
Description: excinuclease ABC subunit B
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|SAAS:SAAS00341756}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 36 43 ATP. {ECO:0000256|HAMAP-Rule:MF_00204}.
Site:
Gene names (primary): uvrB
Gene names (synonym):
Mass: 75,065
Subunit structure [CC]: SUBUNIT: Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587, ECO:0000256|SAAS:SAAS00009162}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; excinuclease ABC activity [GO:0009381]; helicase activity [GO:0004386]; nucleotide-excision repair [GO:0006289]; SOS response [GO:0009432]
Gene ontology IDs: GO:0003677; GO:0004386; GO:0005524; GO:0005737; GO:0006289; GO:0009381; GO:0009432
Chain:
Signal peptide:
Domain [CC]: DOMAIN: The beta-hairpin motif is involved in DNA binding. {ECO:0000256|HAMAP-Rule:MF_00204}.
Sequence similarities: SIMILARITY: Belongs to the UvrB family. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587, ECO:0000256|SAAS:SAAS00538712}.; SIMILARITY: Contains 1 UVR domain. {ECO:0000256|HAMAP-Rule:MF_00204, ECO:0000256|RuleBase:RU003587}.; SIMILARITY: Contains 1 helicase ATP-binding domain. {ECO:0000256|HAMAP-Rule:MF_00204}.; SIMILARITY: Contains 1 helicase C-terminal domain. {ECO:0000256|HAMAP-Rule:MF_00204}.
Protein families: UvrB family
Coiled coil:
Domain [FT]: DOMAIN 23 197 Helicase ATP-binding. {ECO:0000259|PROSITE:PS51192}.; DOMAIN 428 590 Helicase C-terminal. {ECO:0000259|PROSITE:PS51194}.; DOMAIN 619 654 UVR. {ECO:0000259|PROSITE:PS50151}.
Motif: MOTIF 89 112 Beta-hairpin. {ECO:0000256|HAMAP-Rule:MF_00204}.
Region:
EMBL: AGBZ02000003
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92404
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.300;4.10.860.10
InterPro: MF_00204
PANTHER: IPR006935;IPR014001;IPR001650;IPR027417;IPR001943;IPR004807;IPR024759
PIRSF: PTHR24029
PRINTS:
PROSITE:
Pfam: PS51192;PS51194;PS50151
ProDom: PF00271;PF04851;PF02151;PF12344
SMART:
SUPFAM: SM00487;SM00490
TIGRFAMs: SSF46600;SSF52540

25727-27692(-)

>nucleotide sequence
TTATTCTGCTTCAATTTCAATAATAATATCGCGTAATTCTGCAGCTCGTTCAAAATCAAG
TTTTTTACTTGCTTCATACATTTCTTTTCGTAAATCTTGTAGTAATCCTTCTTTAGCTGT
TTTATATTCTTTTGATTTTTTATTTTTAATTTTATGTAATTTATCATCTTGCCGTTTAAT
ATTAGTATAATCACGAATATTCTTAATAATTGTCGTTGGAGTAATATGATATTTCAAATT
ATATGCCGCTTGAATTTGACGTCGACGAGCAGTTTCTTGCATTGCTTTTTCCATTGATTC
AGAAACAGTATCAGCATATAAAATAACATGTCCAGCAGCATTCCTTGCAGCACGTCCAAT
TGTTTGAATTAAACTTCGTTCATTTCGTAAAAATCCTTGCTTATCAGCATCAAGAATACA
AATTAATGATACTTCTGGAATATCAATCCCTTCACGAATTAAATTAACACCAACAATGCA
ATCATATACTCCTTTTCGTAAATCCAATAAGATTTGACTACGTTCTAAAGTTTTTAATTC
ACTATGCAAATATGCAACTTTAACATTTTGTTCTTGTAAATAACTTGTTAAATCTTCTGA
CATACGAATTGTTAAAGTAGTAATAAAAACACGTTCATTTTTTTTGCGGCGTATTTTTAC
TTGCTCAATAATATCATCAATTTGTCCTACTGTTGATTTTACTTCTACTGTCGGATCTAA
TAACCCTGTTGGACGAATAATTTGTTCAACCACTTGGTTATTCACTAAACCTAATTCATA
ATCCCCCGGCGTTGCTGAAACATAAATAACATTTTTTAATTTTTGATTAAATTCATCAAA
ATTTAGGGGGCGATTATCTAATGCACTTTTTAAACGAAAACCATAATTTACTAATGTTTC
TTTTCGACTTCGATCTGTGTTATACATTGCTCGAATTTGCGGTAATGACATATGTGATTC
ATCAATAATAGTTAAAAAATCTTCGCCAAAAAAATCAATCAAAGTATAAGGAGCCTCACC
TTCTTCTCGTAAATCTAAATGTCGTGAATAATTTTCAATTCCAGAACATATCCCACTTTC
ACGGAGTAAATCTAAATCATATTCCGTACGTTGTTTTAAACGTTGTAACTCAACAATTTT
TCCAGCCACTTCTAATTCAACTAAGCGAACCTTTAACTCTGCTTCAATGCGTTTAATTGC
TTCAACTAATCGTTCTGGAGAAGTAATATAATCTTGCGCTGGAAAAATTGTTAATGTTGA
TAACCGCTGTGTAACATCACCAGTGATTCCATCAATATAAACTAAATCATCAATTTCATC
ACCAAACAAATCAATTCGAATAAATGTTTTAACATTTCAGCCTGGAACAATTTTAATCAC
ATCACCTTTAGCACTAAAACTACCTGGAATTAATTCAATATCATTACGAACATATCCTGA
TGATACTAAAAAATTGGCTAAATCTTTTTTACTAATTTTTTGATTACGATTAATTTGTAA
AAAAGATTTTTTATATTCATTTGGATCTTGTGCCCCATAAATGGCGGCAACAGAAGCTAC
GACAATAACATCTTTTCGAGTTGTTAAAGCATTAAAAGCACTTAACCGCATCATGTCTAA
TTCCATATTTTGGCGAGCATCTTTATTAATATATAAATCTTTTCCTGGTAAATAAGCTTC
TGGTTGATAAAAATCAAAGTTTGAAACAAAATACTCAACACGATTTTCTGGAAACATTTC
TTTTAACTCAACATATAGTTGCATTGCTAATGTTTTATTATGAGCCATCACCAATGTTGG
CTTCTGATAATTTTCAATAACATTTGCCATTGTAAATGTTTTTCCAGTTCCGGTTGCTCC
TAACAAAACTTGATGCTTTTTGTTTGCGAATAAATTGGTAGTTAATTTTTCAATTGCCCG
TGGTTGATCACCAGCTGGTAAATAATTAGATGTTAATTTAAACAT

>protein sequence
MFKLTSNYLPAGDQPRAIEKLTTNLFANKKHQVLLGATGTGKTFTMANVIENYQKPTLVM
AHNKTLAMQLYVELKEMFPENRVEYFVSNFDFYQPEAYLPGKDLYINKDARQNMELDMMR
LSAFNALTTRKDVIVVASVAAIYGAQDPNEYKKSFLQINRNQKISKKDLANFLVSSGYVR
NDIELIPGSFSAKGDVIKIVPGWNVKTFIRIDLFGDEIDDLVYIDGITGDVTQRLSTLTI
FPAQDYITSPERLVEAIKRIEAELKVRLVELEVAGKIVELQRLKQRTEYDLDLLRESGIC
SGIENYSRHLDLREEGEAPYTLIDFFGEDFLTIIDESHMSLPQIRAMYNTDRSRKETLVN
YGFRLKSALDNRPLNFDEFNQKLKNVIYVSATPGDYELGLVNNQVVEQIIRPTGLLDPTV
EVKSTVGQIDDIIEQVKIRRKKNERVFITTLTIRMSEDLTSYLQEQNVKVAYLHSELKTL
ERSQILLDLRKGVYDCIVGVNLIREGIDIPEVSLICILDADKQGFLRNERSLIQTIGRAA
RNAAGHVILYADTVSESMEKAMQETARRRQIQAAYNLKYHITPTTIIKNIRDYTNIKRQD
DKLHKIKNKKSKEYKTAKEGLLQDLRKEMYEASKKLDFERAAELRDIIIEIEAE