SPM_004560

Uniprot: A0A037ULW5
Description: Uracil phosphoribosyltransferase
EC number: 2.4.2.9
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00084446}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01218}; ; Note=Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP. {ECO:0000256|HAMAP-Rule:MF_01218}
Enzyme regulation: ENZYME REGULATION: Allosterically activated by GTP. {ECO:0000256|HAMAP-Rule:MF_01218}.
Function [CC]: FUNCTION: Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate. {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00296571}.
Pathway: PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00084648}.
Active site:
Binding site: BINDING 77 77 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_01218}.; BINDING 102 102 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_01218}.; BINDING 192 192 Uracil; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_01218}.; BINDING 198 198 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_01218}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): upp
Gene names (synonym):
Mass: 23,106
Subunit structure [CC]:
Gene ontology (GO): GTP binding [GO:0005525]; magnesium ion binding [GO:0000287]; uracil phosphoribosyltransferase activity [GO:0004845]; UMP salvage [GO:0044206]; uracil salvage [GO:0006223]
Gene ontology IDs: GO:0000287; GO:0004845; GO:0005525; GO:0006223; GO:0044206
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the UPRTase family. {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00569882}.
Protein families: UPRTase family
Coiled coil:
Domain [FT]: DOMAIN 5 206 PRTase-like. {ECO:0000259|Pfam:PF14681}.
Motif:
Region: REGION 129 137 5-phospho-alpha-D-ribose 1-diphosphate binding. {ECO:0000256|HAMAP-Rule:MF_01218}.; REGION 197 199 Uracil binding. {ECO:0000256|HAMAP-Rule:MF_01218}.
EMBL: AGBZ02000003
ProteinModelPortal: A0A037ULW5
MEROPS:
EnsemblBacteria KO: KAI92442
UniPathway:
CDD:
Gene3D: cd06223
HAMAP: 3.40.50.2020
InterPro: MF_01218_B
PANTHER: IPR000836;IPR029057;IPR005765
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF14681
SMART:
SUPFAM:
TIGRFAMs: SSF53271

68839-69463(-)

>nucleotide sequence
TTATTTAGTTCCAAAAATACGGTCTCCTGCATCACCTAGGCCAGGAATAATATAACCATG
TTCATCCAAGCGTTCGTCTAATGCAGCAACATAAATATCAACATCTGGATGATGTTCTTC
AACGCATTTTTTTCCTTCAGGTGCAGCTACCAAACATACTAATTTAATATTAGTTGCTCC
TCATTCTTTAACTTTTTGAATTGCAAGATTAGCACTGCCCCCTGTTGCTAACATTGGATC
TAAAACTAAAACATTTGCTTTATTAATATTTTCTGGTTTTTTGGCAAAATATTCATGTGG
TTCTAAGGTTTTTTCATCTCGGTATAATCCAATATGTCCAATTTTAGCATTTGGGACTAA
ATTAATAATTCCATCAACCATCCCCAATCCTGCTCTTAAAATTGGAAATAAAACAATATC
ATTTGCAATTTTATATCCCTTTGTTTTTGCTACTGGTGTTTCAATTTCAAATTCATTTAA
TTCAATATTTCGAAATATTTCATACGCCATTAATTGCGCAATTTCATCTAAATTTTCTTT
AAAGACCTTTGAATTACTTTCCTTTTTGCGCATTCTTGTTAATTTATCCAAAATTAACGG
GTGTTTTATTACAGTAAATGACAT

>protein sequence
MSFTVIKHPLILDKLTRMRKKESNSKVFKENLDEIAQLMAYEIFRNIELNEFEIETPVAK
TKGYKIANDIVLFPILRAGLGMVDGIINLVPNAKIGHIGLYRDEKTLEPHEYFAKKPENI
NKANVLVLDPMLATGGSANLAIQKVKEWGATNIKLVCLVAAPEGKKCVEEHHPDVDIYVA
ALDERLDEHGYIIPGLGDAGDRIFGTK