SPM_004665

Uniprot: A0A037UKK6
Description: cysteinyl-tRNA synthetase
EC number: 6.1.1.16
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). {ECO:0000256|HAMAP-Rule:MF_00041}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00041}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00041}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site: BINDING 271 271 ATP. {ECO:0000256|HAMAP-Rule:MF_00041}.
Calcium binding:
DNA binding:
Metal binding: METAL 25 25 Zinc. {ECO:0000256|HAMAP-Rule:MF_00041}.; METAL 210 210 Zinc. {ECO:0000256|HAMAP-Rule:MF_00041}.; METAL 236 236 Zinc. {ECO:0000256|HAMAP-Rule:MF_00041}.; METAL 240 240 Zinc. {ECO:0000256|HAMAP-Rule:MF_00041}.
Nucleotide binding:
Site:
Gene names (primary): cysS
Gene names (synonym):
Mass: 51,746
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00041}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; cysteine-tRNA ligase activity [GO:0004817]; zinc ion binding [GO:0008270]; cysteinyl-tRNA aminoacylation [GO:0006423]
Gene ontology IDs: GO:0004817; GO:0005524; GO:0005737; GO:0006423; GO:0008270
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00041}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 14 314 tRNA-synt_1e. {ECO:0000259|Pfam:PF01406}.
Motif: MOTIF 27 37 "HIGH" region. {ECO:0000256|HAMAP-Rule:MF_00041}.; MOTIF 268 272 "KMSKS" region. {ECO:0000256|HAMAP-Rule:MF_00041}.
Region:
EMBL: AGBZ02000004
ProteinModelPortal: A0A037UKK6
MEROPS:
EnsemblBacteria KO: KAI92032
UniPathway:
CDD:
Gene3D: cd00672
HAMAP: 3.40.50.620
InterPro: MF_00041
PANTHER: IPR015803;IPR024909;IPR014729;IPR032678;IPR009080
PIRSF: PTHR10890
PRINTS:
PROSITE: PR00983
Pfam:
ProDom: PF01406
SMART:
SUPFAM:
TIGRFAMs: SSF47323

4956-6294(+)

>nucleotide sequence
ATGAAATTATATAATACTTTGACTCGTAATTTTACAGATTATAGTCAAACAAAAAAAATT
AATATTTATGGTTGTGGACCAACTGTCTATAATTACATTCATATTGGTAATGCTCGCGCA
ATTATTACTGTTGATTTATTAGTTAGTTTTTTACAATTTCAACAAATTGAGGTTAATTAT
ATTCAAAATTATACGGATATTGATGATAAGATTATTGCTAAAGCAGCAGCAGAACATAAA
ACTGAACAAGAGATTGCAGAATTTTATATTAAAGCTTTTGAAGAAGATGTTTTTAATTTA
AATGTTAGAACACCAACTAAGCGAGTACGAGTAACAGACCATGTTAAAGATATTGTTACT
TTTATTCAAAAATTAGTTAAAATTAATGCTGCATATGAAGTTAATGGTAGTGTATATTTT
GATATTGCAAAATATCAAACACAATATGGACAATTAGCTAATAAAAAAATTTCAGAATTA
GAAGCTAATGCTCGAATTGAACATGATGCTAACAAACATAGTCAATATGACTTTGCATTA
TGAAAGCAAACAACAGAAGGGATTTCTTTCCCCTTTTATGATCAAAAAGGTAAATTATAT
CAAGGTCGTCCAGGATGACATACGGAATGTGCTGTCTTAATTGATAAATTTTTTCATAAA
GAAACAATTGACATTCATGCCGGAGGAATTGATTTAGTTTTTCCTCATCATGAAAATGAA
CGAATTCAATTTTTCGCAAAAAATAATAAGGAAATTGCAAAAATTTGAATGCATAATGGG
CATTTAAATGTTGCTGATAAAAAAATGAGTAAATCTTTAAATAATACTATTTTAGTTCGT
GATTTTATTAAATTATATGGGGCAAATACATTACGTTACTTATTATATGCAACTAATTAT
ACACAACCATTAAATGTAACAGAAACTTTAATTATTAGTGCGCAAGGTGAAACAGAAAAG
ATTTTTAAAGTGTTAAAAGCAATAAATTTATATTTAGCTGAATATGATTTAAATTATAAT
TTAACACAACATGGTCACAATGTTAAGGAAGTCCTTGCTGAGTTAGCAAATAATTTGAAT
AGCCCGAATGTTTTAACTATTATTAGTAAAATGACTAAAATTATTAATACTCAATTACGT
GACAAAAAACTTAATTTACAACTTGCTAGTGATTTTTATAATATTATTTTTAATATTATG
AATTTTAATTTTAAATTACCAGTAATTAGTAGTGAAATTCGTAAATATTTATTAGAATGA
ATTAAGTCAAAAAATAATAAGGATTTTGTCAAAGCAGATGAACTTCGTAAGGTTTTAGTA
GAAAAAGATATTTTGTAA

>protein sequence
MKLYNTLTRNFTDYSQTKKINIYGCGPTVYNYIHIGNARAIITVDLLVSFLQFQQIEVNY
IQNYTDIDDKIIAKAAAEHKTEQEIAEFYIKAFEEDVFNLNVRTPTKRVRVTDHVKDIVT
FIQKLVKINAAYEVNGSVYFDIAKYQTQYGQLANKKISELEANARIEHDANKHSQYDFAL
WKQTTEGISFPFYDQKGKLYQGRPGWHTECAVLIDKFFHKETIDIHAGGIDLVFPHHENE
RIQFFAKNNKEIAKIWMHNGHLNVADKKMSKSLNNTILVRDFIKLYGANTLRYLLYATNY
TQPLNVTETLIISAQGETEKIFKVLKAINLYLAEYDLNYNLTQHGHNVKEVLAELANNLN
SPNVLTIISKMTKIINTQLRDKKLNLQLASDFYNIIFNIMNFNFKLPVISSEIRKYLLEW
IKSKNNKDFVKADELRKVLVEKDIL