SPM_004910

Uniprot: A0A037UJA5
Description: 50S rRNA methyltransferase
EC number: 2.1.1.177
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00094207}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00094219}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rlmH
Gene names (synonym):
Mass: 18,023
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00094194}.
Gene ontology (GO): cytoplasm [GO:0005737]; rRNA (pseudouridine-N3-)-methyltransferase activity [GO:0070038]
Gene ontology IDs: GO:0005737; GO:0070038
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA methyltransferase RlmH family. {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS00573467}.
Protein families: RNA methyltransferase RlmH family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal: A0A037UJA5
MEROPS:
EnsemblBacteria KO: KAI92079
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.1280.10
InterPro: MF_00658
PANTHER: IPR029028;IPR003742;IPR029026
PIRSF:
PRINTS: PIRSF004505
PROSITE:
Pfam:
ProDom: PF02590
SMART:
SUPFAM:
TIGRFAMs: SSF75217

55760-56231(+)

>nucleotide sequence
ATGGATATTAAAATTCTGGCAATTGGTTCATTAGATAAAAAGTATTTGGTAATAGGTTGT
GAAACTTTTCTTGAACGAATTAAGCATTATGCAAAATTAGAAATTATTGAATTAAAAGAA
GTTAACAATAAAACTGAACAAGTAACAATTAATGAACAAACGGCATTAGTAATAAAAAAA
CTATTAGATTATCCGGAATATTATAAAATATTATTAGCCTCACAAGGACAAATATTAACA
AGTGAACAAATTGCTGCATACATTACGGAAGTTAAAGATTTTAAAAAAGCAAAGTTATTA
TTTATTATTGGGGGTAGTCATGGTTTTAATAATAATGAACTATTACCAAAAGTTAATTTT
CACCTGAGTTTTGGAGCAATAACTTTACCGCATCAATTATGTCGATTAGTATTATTAGAA
CAAATATATCGTAGTTTTAAGATAATTAATAATGAAATTTATCATAAGTAA

>protein sequence
MDIKILAIGSLDKKYLVIGCETFLERIKHYAKLEIIELKEVNNKTEQVTINEQTALVIKK
LLDYPEYYKILLASQGQILTSEQIAAYITEVKDFKKAKLLFIIGGSHGFNNNELLPKVNF
HLSFGAITLPHQLCRLVLLEQIYRSFKIINNEIYHK