SPM_005090

Uniprot: A0A037UQV7
Description: protein kinase
EC number: 2.3.1.234
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA. {ECO:0000256|HAMAP-Rule:MF_01445, ECO:0000256|SAAS:SAAS00018958}.
Cofactor: COFACTOR: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000256|HAMAP-Rule:MF_01445}; ; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01445}
Enzyme regulation:
Function [CC]: FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. {ECO:0000256|HAMAP-Rule:MF_01445, ECO:0000256|SAAS:SAAS00195370}.
Pathway:
Active site:
Binding site: BINDING 165 165 Substrate. {ECO:0000256|HAMAP-Rule:MF_01445}.; BINDING 178 178 Substrate; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_01445}.; BINDING 182 182 Substrate. {ECO:0000256|HAMAP-Rule:MF_01445}.; BINDING 270 270 Substrate. {ECO:0000256|HAMAP-Rule:MF_01445}.
Calcium binding:
DNA binding:
Metal binding: METAL 110 110 Iron. {ECO:0000256|HAMAP-Rule:MF_01445}.; METAL 114 114 Iron. {ECO:0000256|HAMAP-Rule:MF_01445}.; METAL 297 297 Iron. {ECO:0000256|HAMAP-Rule:MF_01445}.
Nucleotide binding:
Site:
Gene names (primary): tsaD
Gene names (synonym):
Mass: 34,751
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; iron ion binding [GO:0005506]; kinase activity [GO:0016301]; metalloendopeptidase activity [GO:0004222]; N(6)-L-threonylcarbamoyladenine synthase [GO:0061711]; tRNA threonylcarbamoyladenosine modification [GO:0002949]
Gene ontology IDs: GO:0002949; GO:0004222; GO:0005506; GO:0005737; GO:0016301; GO:0061711
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the KAE1 / TsaD family. {ECO:0000256|HAMAP-Rule:MF_01445, ECO:0000256|SAAS:SAAS00542034}.
Protein families: KAE1 / TsaD family
Coiled coil:
Domain [FT]: DOMAIN 22 303 Peptidase_M22. {ECO:0000259|Pfam:PF00814}.
Motif:
Region: REGION 132 136 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_01445}.
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92115
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_01445
PANTHER: IPR000905;IPR017861;IPR022450
PIRSF:
PRINTS:
PROSITE: PR00789
Pfam:
ProDom: PF00814
SMART:
SUPFAM:
TIGRFAMs:

77897-78854(+)

>nucleotide sequence
ATGGTAATTCTTGCAATTGAGACAAGCTGTGACGAAACTAGTATTGCGATTTTTAAGAAT
GGAAAAATTTTAGCAAATATTATTTCGTCACAAATTAAAGAACATACAAAGTATGGCGGA
GTTGTTCCAGAACTTGCTTCGCGGTTACATTTAAAAAACTTTTCTTATGTTTTACTTGAA
GCTTTGCAAAAAGCTAAGATTAATATAACAGAGTTACAATACATTGCATACACAGCAAAA
CCAGGTTTAATTGGAGCATTACATATTGGGAAAATAATAGCAGAAACATTGTCTAATTAT
TTAGAAATTCCAATTTTACCTTTAAACCATTTATATGGTCATATCTATGCTAGTGCAATC
AATAATGATTTTTTATTTCCTCTTTTAGCAGTTCTAGTTAGTGGTGGCCATACTCAGTTA
ATATTGATGAAAAAACATTTGAATTTTGAAATTTTAGGCTCAACATTGGATGATGCAATT
GGTGAATGTTATGATAAGGTTGCCCGAATGTTGGACTTAGGGTATCCGGGCGGGCCAGTT
ATTGATAAGTTAGCAGCGACAGGTACAACAACACAATATCACTTACCATTACCATTAAAT
GACCAAAGTTATAATTTCTCTTTTAGCGGATTAAAATCAGCCGTGGCTAATTTAATTGTA
AAAGAACAACGTAATACTAAAATTAACCTGTCTAATTTTTGCGCGACATTTCAAAAAACA
TGTGTTGATATTATTATGCGAAAAACAAAATTAGCAATTCAAAATTTTCAACCGCAAATG
GTTACGTTAGTCGGTGGGGTTTCAGCTAATAGCGCATTACGAGCAGCAATTTTAAATTTG
GCAGCACCAAATTTAAAAGTCATTATTCCAAAATTAGAATATTGTACGGATAATGCTGCA
ATGATTGCTCGGTTAGCAGCACAAGAACTACTGGAAAACAACATAAAAGGAGACTAG

>protein sequence
MVILAIETSCDETSIAIFKNGKILANIISSQIKEHTKYGGVVPELASRLHLKNFSYVLLE
ALQKAKINITELQYIAYTAKPGLIGALHIGKIIAETLSNYLEIPILPLNHLYGHIYASAI
NNDFLFPLLAVLVSGGHTQLILMKKHLNFEILGSTLDDAIGECYDKVARMLDLGYPGGPV
IDKLAATGTTTQYHLPLPLNDQSYNFSFSGLKSAVANLIVKEQRNTKINLSNFCATFQKT
CVDIIMRKTKLAIQNFQPQMVTLVGGVSANSALRAAILNLAAPNLKVIIPKLEYCTDNAA
MIARLAAQELLENNIKGD