SPM_005150

Uniprot: A0A037UNE0
Description: tRNA (guanine-N1)-methyltransferase
EC number: 2.1.1.228
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|RuleBase:RU003464, ECO:0000256|SAAS:SAAS00014942}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically methylates guanosine-37 in various tRNAs. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|RuleBase:RU003464, ECO:0000256|SAAS:SAAS00014913}.
Pathway:
Active site:
Binding site: BINDING 112 112 S-adenosyl-L-methionine; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|PIRSR:PIRSR000386-1}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): trmD
Gene names (synonym):
Mass: 27,969
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|RuleBase:RU003464, ECO:0000256|SAAS:SAAS00014946}.
Gene ontology (GO): cytoplasm [GO:0005737]; tRNA (guanine(37)-N(1))-methyltransferase activity [GO:0052906]
Gene ontology IDs: GO:0005737; GO:0052906
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA methyltransferase TrmD family. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|RuleBase:RU003464, ECO:0000256|SAAS:SAAS00540615}.
Protein families: RNA methyltransferase TrmD family
Coiled coil:
Domain [FT]: DOMAIN 25 218 tRNA_m1G_MT. {ECO:0000259|Pfam:PF01746}.
Motif:
Region: REGION 131 136 S-adenosyl-L-methionine binding. {ECO:0000256|HAMAP-Rule:MF_00605, ECO:0000256|PIRSR:PIRSR000386-1}.
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92126
UniPathway:
CDD:
Gene3D:
HAMAP: 1.10.1270.20;3.40.1280.10
InterPro: MF_00605
PANTHER: IPR029028;IPR002649;IPR023148;IPR029026;IPR016009
PIRSF:
PRINTS: PIRSF000386
PROSITE:
Pfam:
ProDom: PF01746
SMART:
SUPFAM:
TIGRFAMs: SSF75217

86427-87159(+)

>nucleotide sequence
ATGAAAAAGTTTACAGTATTAACTTTATTTCCAGAAATGTTTAATAATTTTATGACAACA
TCAATTATTAAAAAAGCACTTTGTGAAAAGATGATTACAATTAAAATTGTTGATATTCGT
GATTATAGCATTGGCAAACATCATCAAGTTGATGATTACCAATATGGTGGTGGTGAAGGC
ATGGTTTTAATGATTGAACCATTAGTAGCAGCGATTAAAGCTCACCAAACAAAAGACAGT
CTAACAATTTTGTTAACACCACAAGGGAAACAATATGCTCAGGAACAAGTGCAACAATTT
GCAGCAAATAATAATGATTTAATCTTAGTTTGTGGGCATTATGAAGGCTTTGATGAAAGA
GTTTTATATTATATTGATTACGAATTATCAATTGGTGATTATATTTTAACTGGTGGTGAA
TTAGCTAGTATGGTTGTAATTGATAGTGTCACTCGTTTATGTGAAAATGTAATTAATACA
CAATCACATTTAAATGATTCTTTTTCTAACAATCTATTAGATTATCCTGTTTATACAAAA
CCAGTTGAATATGATGGACATTATGTTCCTGAAGTTTTATTAAGTGGTCATCATCAGAAA
ATTGCCCAATGACGAGAATATGAAGCATTAAAAAAAACTTGATTAAAACGTCCTGATCTT
TTAGAAAAAAAAGTTTTAACCACAGAACAACAAATATTATTAGAAAAAATTAAAAGCGAA
AAAAAGGTTTAA

>protein sequence
MKKFTVLTLFPEMFNNFMTTSIIKKALCEKMITIKIVDIRDYSIGKHHQVDDYQYGGGEG
MVLMIEPLVAAIKAHQTKDSLTILLTPQGKQYAQEQVQQFAANNNDLILVCGHYEGFDER
VLYYIDYELSIGDYILTGGELASMVVIDSVTRLCENVINTQSHLNDSFSNNLLDYPVYTK
PVEYDGHYVPEVLLSGHHQKIAQWREYEALKKTWLKRPDLLEKKVLTTEQQILLEKIKSE
KKV