SPM_005170

Uniprot: A0A037UQW8
Description: ribonuclease HII
EC number: 3.1.26.4
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester. {ECO:0000256|HAMAP-Rule:MF_00052, ECO:0000256|RuleBase:RU003515, ECO:0000256|SAAS:SAAS00016988}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|SAAS:SAAS00608041}; ; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|HAMAP-Rule:MF_00052}; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00052}; ; Note=Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding. {ECO:0000256|HAMAP-Rule:MF_00052}
Enzyme regulation:
Function [CC]: FUNCTION: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. {ECO:0000256|HAMAP-Rule:MF_00052, ECO:0000256|RuleBase:RU003515, ECO:0000256|SAAS:SAAS00017026}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 24 24 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00052}.; METAL 25 25 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00052}.; METAL 116 116 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00052}.
Nucleotide binding:
Site:
Gene names (primary): rnhB
Gene names (synonym):
Mass: 23,419
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; manganese ion binding [GO:0030145]; RNA binding [GO:0003723]; RNA-DNA hybrid ribonuclease activity [GO:0004523]; RNA catabolic process [GO:0006401]
Gene ontology IDs: GO:0003723; GO:0004523; GO:0005737; GO:0006401; GO:0030145
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNase HII family. {ECO:0000256|HAMAP-Rule:MF_00052, ECO:0000256|RuleBase:RU003515, ECO:0000256|SAAS:SAAS00541516}.
Protein families: RNase HII family
Coiled coil:
Domain [FT]: DOMAIN 21 194 RNase_HII. {ECO:0000259|Pfam:PF01351}.
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92130
UniPathway:
CDD:
Gene3D:
HAMAP: 3.30.420.10
InterPro: MF_00052_B
PANTHER: IPR022898;IPR001352;IPR024567;IPR012337
PIRSF: PTHR10954
PRINTS:
PROSITE:
Pfam:
ProDom: PF01351
SMART:
SUPFAM:
TIGRFAMs: SSF53098

89389-90019(+)

>nucleotide sequence
TTAATTATGAATAAATTTGAAAAAGAGATTCTAATATCATATCCTAATACAACAATTCTG
GCAGGAACTGATGAAGCTGGTCGCGGATGCTTAGCAGGTCCTTTAATTGTTAGTGCTGTT
ATTTTACCACTAGATTATGTTAACGATGAAATTAAGGATAGTAAAAAATTAACAGCAAAA
CAACGAATTAAATTAGCAGATGAAATTATGTCCATTGCAATTAGTTATACGATTGAAATT
ATTTCTGTTGCAATGGTTGAAGAATTGAATCCCAAGCAAGCAAGTATTTTTGGAATGCAA
CAAGCAATTGACAACTTAATGGTTAAACCAGATATAATTTTAACTGATGCAGAAAAGTTA
GGACCTTCTTATCATCACCAAGCAATTATTAAAGGAGATAGTCTTTCGCAATCAATTGCG
GCAGCTTCAATTTTAGCAAAAGTAACAAGAGATAATTTAATGCTCAAATTTGATCAGGAC
TATCCACAGTATGATTTTAAAAATAATAAAGGATACGGAACCAAAAAACATTTATTAGCA
TTGCAACAATTTGGAATTACATCATTGCATCGGAAAACATATCGTCCCGTTCGTGAAACT
ATGTTAAAAAATAATGTTAATTATAAATAA

>protein sequence
LIMNKFEKEILISYPNTTILAGTDEAGRGCLAGPLIVSAVILPLDYVNDEIKDSKKLTAK
QRIKLADEIMSIAISYTIEIISVAMVEELNPKQASIFGMQQAIDNLMVKPDIILTDAEKL
GPSYHHQAIIKGDSLSQSIAAASILAKVTRDNLMLKFDQDYPQYDFKNNKGYGTKKHLLA
LQQFGITSLHRKTYRPVRETMLKNNVNYK