SPM_005185

Uniprot: A0A037UKW4
Description: GMP synthase
EC number: 6.3.5.2
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + XMP + L-glutamine + H(2)O = AMP + diphosphate + GMP + L-glutamate. {ECO:0000256|HAMAP-Rule:MF_00344, ECO:0000256|SAAS:SAAS00496552}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the synthesis of GMP from XMP. {ECO:0000256|HAMAP-Rule:MF_00344, ECO:0000256|SAAS:SAAS00314024}.
Pathway: PATHWAY: Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. {ECO:0000256|HAMAP-Rule:MF_00344, ECO:0000256|SAAS:SAAS00496560}.
Active site: ACT_SITE 83 83 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_00344}.; ACT_SITE 169 169 {ECO:0000256|HAMAP-Rule:MF_00344}.; ACT_SITE 171 171 {ECO:0000256|HAMAP-Rule:MF_00344}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 223 229 ATP. {ECO:0000256|HAMAP-Rule:MF_00344}.
Site:
Gene names (primary): guaA
Gene names (synonym):
Mass: 57,309
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00344, ECO:0000256|SAAS:SAAS00008735}.
Gene ontology (GO): ATP binding [GO:0005524]; GMP synthase (glutamine-hydrolyzing) activity [GO:0003922]; pyrophosphatase activity [GO:0016462]; glutamine metabolic process [GO:0006541]; GMP biosynthetic process [GO:0006177]
Gene ontology IDs: GO:0003922; GO:0005524; GO:0006177; GO:0006541; GO:0016462
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain. {ECO:0000256|HAMAP-Rule:MF_00344}.; SIMILARITY: Contains 1 glutamine amidotransferase type-1 domain. {ECO:0000256|HAMAP-Rule:MF_00344}.
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 6 195 Glutamine amidotransferase type-1. {ECO:0000259|PROSITE:PS51273}.; DOMAIN 196 387 GMPS ATP-PPase (ATP pyrophosphatase). {ECO:0000259|PROSITE:PS51553}.
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92132
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.620;3.40.50.880
InterPro: MF_00344
PANTHER: IPR029062;IPR017926;IPR001674;IPR004739;IPR022955;IPR025777;IPR022310;IPR014729
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51273;PS51553
ProDom: PF00117;PF00958;PF02540
SMART:
SUPFAM:
TIGRFAMs: SSF52317

91765-93304(+)

>nucleotide sequence
ATGAAAACAGCAAATAAAATTATTATTTTAGATTTTGGGTCACAATATACTCAATTAATT
GCCCGTCGAATTCGTGACTTAGAGGTCTATTGTGAAGTATGACCGTATAATACAACATTA
GAAAAAATTAAAACAACACCAATGAAAGGGATTATTTTATCAGGTGGCCCTGCTTCTGTT
TATGCAGAAGATGCATTTATAATTGATAAAAAATTATTTGAATTAGAAGTTCCTATTTTA
GGAATTTGTTATGGAATGCAATTAATAAGTCATCTGCATGGAGGCACAGTACAACGAGCA
ACAAAACAAGAATTTGGTTTTTCAGAATTAATTATTGATAATCAAGAAGATTTGTTTGCT
AAGGTTCCTATTAAATCACAAGTATGAATGAGCCATGCTGACCACATTGAAATAATGCCA
ACAGATTTTATTCAAATTGCTCATAGCGAAAATTCAATTAGTGCAATTAAACATTCAGAA
AAGAAAATTTATGGTTTACAATTTCACCCTGAAGTAACTCACACTTTAATTGGTGAACAA
TTATTAAGCAATTTTGTTTTTAATATTTGTGGTTGCGAACCAACATGAAAAATTACAGAG
TTTATTTCAACAGCAACAACCGAAATTAAAGGTAAGGTTGGAAGCGATAATGTTGTTTTA
GCATTATCAGGCGGTGTTGATTCTAGTGTTTGTGCAGTTTTATTACATAAAGCAATTGGG
AAACAATTAACATGTATTTTTGTTGACACCGGATTGTTACGACAAGATAGTGGATGAAAT
GATTTACAAAAATTTCAAGAAAAATTTAAATTAAATATTATTAAAATTGATGCTCAAGAA
AGATTTTTAACTGCCTTAAAGGGAGTTACTAATCCAGAACAAAAACGAAAAATTATTGGA
AATTTATTTATTGAGATTTTTAATGAAGAAGCGATAAAAATTAAAAATGTTAAATGATTA
GGACAAGGAACAATTTATCCAGATGTTATTGAATCGATTTCCGTAAAGGGACCCTCTGCC
ACAATTAAATCTCATCATAATGTTGGGGGATTACCAAAAGATTTACCATTTCAATTAATT
GAACCATTACGCGAATTGTTTAAAGACGAAGTTCGCCGAACTGGCGAAGCATTGGGAATT
GATTCGAAATTTGTGTACAAGCATCCTTTTCCGGGACCAGGATTAGCAGTCCGCATTATT
GGTGAAATTACAGCAGAGAAAGTAGCCTTATTACAAGCAGCTGATCAAATTTTTATTGAT
GAATTATATCAAGCAAATTTATATGATCAAGTTGCACAAGCATTTGTTGTTTTATTACCT
GTTCAGTCGGTTGGTGTAATGGGCGATGTGCGAACATATGGATACACTGCTGTTGTGCGT
AGTGTTGATACAACTGATTTTATGACGGCAAATTGAAGTCGTTTACCATTTGAATTATTG
GAAAAAGTATCAACTCGAATTGTAAGCGAAGTGCATGGAATTAATCGAGTTACTTATGAT
ATTACATCAAAACCACCAGGAACAATTGAATGAGAATAA

>protein sequence
MKTANKIIILDFGSQYTQLIARRIRDLEVYCEVWPYNTTLEKIKTTPMKGIILSGGPASV
YAEDAFIIDKKLFELEVPILGICYGMQLISHLHGGTVQRATKQEFGFSELIIDNQEDLFA
KVPIKSQVWMSHADHIEIMPTDFIQIAHSENSISAIKHSEKKIYGLQFHPEVTHTLIGEQ
LLSNFVFNICGCEPTWKITEFISTATTEIKGKVGSDNVVLALSGGVDSSVCAVLLHKAIG
KQLTCIFVDTGLLRQDSGWNDLQKFQEKFKLNIIKIDAQERFLTALKGVTNPEQKRKIIG
NLFIEIFNEEAIKIKNVKWLGQGTIYPDVIESISVKGPSATIKSHHNVGGLPKDLPFQLI
EPLRELFKDEVRRTGEALGIDSKFVYKHPFPGPGLAVRIIGEITAEKVALLQAADQIFID
ELYQANLYDQVAQAFVVLLPVQSVGVMGDVRTYGYTAVVRSVDTTDFMTANWSRLPFELL
EKVSTRIVSEVHGINRVTYDITSKPPGTIEWE